bioimagesUnknownSebastian TreuschThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201108146JCB

Electron microscopy of a cryofixed [RNQ+] Rnq1-overexpressing yeast cell containing unduplicated spindle pole bodies (SPBs). The unduplicated SPBs in arrested [RNQ+] cells exhibited a range of abnormal morphologies. The SPBs lacked solid central plaques and often had reduced outer plaques (phenotypes 1 and 2). Some arrested SPBs presented with long half-bridges (phenotype 3) or had a bilobed morphology (phenotype 4 (shown here)).

Electron microscopy of a cryofixed [rnq-] Rnq1-overexpressing yeast cell containing normal elongated spindles.

Electron microscopy of a cryofixed [RNQ+] Rnq1-overexpressing yeast cell containing unduplicated spindle pole bodies (SPBs). The unduplicated SPBs in arrested [RNQ+] cells exhibited a range of abnormal morphologies. The SPBs lacked solid central plaques and often had reduced outer plaques (phenotypes 1 and 2 (shown here)). Some arrested SPBs presented with long half-bridges (phenotype 3) or had a bilobed morphology (phenotype 4).

Electron microscopy of a cryofixed [RNQ+] Rnq1-overexpressing yeast cell showing that [RNQ+] cells arrest with an unduplicated spindle pole body.

Electron microscopy of a cryofixed [RNQ+] Rnq1-overexpressing yeast cell containing unduplicated spindle pole bodies (SPBs). The unduplicated SPBs in arrested [RNQ+] cells exhibited a range of abnormal morphologies. The SPBs lacked solid central plaques and often had reduced outer plaques (phenotypes 1 (shown here) and 2). Some arrested SPBs presented with long half-bridges (phenotype 3) or had a bilobed morphology (phenotype 4).

Electron microscopy of a cryofixed [RNQ+] Rnq1-overexpressing yeast cell containing unduplicated spindle pole bodies (SPBs). The unduplicated SPBs in arrested [RNQ+] cells exhibited a range of abnormal morphologies. The SPBs lacked solid central plaques and often had reduced outer plaques (phenotypes 1 and 2). Some arrested SPBs presented with long half-bridges (phenotype 3 (shown here)) or had a bilobed morphology (phenotype 4).

bioimagesImage 134273 (Figure 3 - C)Image 134259 (Figure 3 - B)Image 134265 (Figure 3 - C)Image 134269 (Figure 3 - C)Figure 3 - BFigure 3 - CImage 134280 (Figure 3 - B)Image 134266 (Figure 3 - C)Image 134274 (Figure 3 - C)Image 134261 (Figure 3 - C)Image 134270 (Figure 3 - C)Image 134267 (Figure 3 - C)Image 134260 (Figure 3 - B)Image 134278 (Figure 3 - B)Image 134262 (Figure 3 - C)Image 134275 (Figure 3 - C)Image 134271 (Figure 3 - C)Image 134279 (Figure 3 - B)Image 134277 (Figure 3 - C)Image 134264 (Figure 3 - C)Image 134263 (Figure 3 - C)Image 134276 (Figure 3 - C)Image 134268 (Figure 3 - C)Image 134272 (Figure 3 - C)Figure 3Sebastian TreuschSusan LindquistfalseAn intrinsically disordered yeast prion arrests the cell cycle by sequestering a spindle pole body component2012-04-23T11:22:26Z2018-11-29T11:22:26Z2018-11-29T11:22:26ZS-JCBD-20110814610.1083/jcb.201108146