bioimagesUnknownMaría Verónica BaezThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201108159JCB

Smaug 1 (green) foci associate with Synapsin clusters (red) in the adult rat hippocampus. 60% of synapses of the CA3 region contain S-foci. A rabbit polyclonal anti-Smaug1 serum (Baez and Boccaccio, JBC, 2005) was used. Tubulin is depicted in blue.

Primary hippocampal neurons were treated with a non-targeting siRNA. Synapsin is shown in green, and tubulin BIII is in blue.

Smaug1 ECFP colocalizes with PBs in U2OS cells. The PB markers Dcp1a (red) and Hedls (blue) were immunostained. Most Smaug1-foci (S-foci) colocalize or are in close contact with P-bodies. Smaug1-ECFP is depicted in green.

Smaug 1 knockdown affects synapse size and number. Two out of four sequences against rat Samug 1 (siSmaug1c and siSmaug1 d) elicit the effect. The treatment with siSmaug1b (no effect) is shown. Synapsin is shown in green, and tubulin BIII is in blue.

Primary hippocampal neurons were exposed to a 60-min NMDA pulse. Protein synthesis was evaluated in situ before and after the pulse with the Click It reagents HPG (green) or AHA (red), respectively, using the FUNCAT strategy.

Smaug1 ECFP colocalizes with P-bodies in U2OS cells. The PB markers Dcp1a (red) and Rck/p54 (blue) were immunostained. Most Smaug1-foci (S-foci) colocalize or are in close contact with P-bodies. Smaug1-ECFP is depicted in green.

Primary neurons were treated with siRNA against the P-body component Rck/p54 and the P-bodies visualized by staining of Rck/p54 (green). Map2 is depicted in green. A significant reduction in P-bodies is observed.

Smaug 1 (green) forms foci in the somatodendritic compartment of the adult rat hippocampus (CA1 region). A rabbit polyclonal anti-human Smaug1 serum (Baez and Boccaccio, JBC, 2005) was used. Tubulin is depicted in blue.

Primary neurons were treated with a non-targeting RNA and the P-bodies visualized by staining of Rck/p54 (green). Map2 is depicted in green.

Smaug 1 knockdown affects synapse size and number. Two out of four sequences against rat Samug 1 (siSmaug1c and siSmaug1 d) elicit the effect. The treatment with siSmaug1d (no effect) is shown. Synapsin is shown in green, and tubulin BIII is in blue.

Smaug 1 knockdown affects synapse size and number. Two out of four sequences against rat Samug 1 (siSmaug1c and siSmaug1 d) elicit the effect. The treatment with siSmaug1c (no effect) is shown. Synapsin is shown in green, and tubulin BIII is in blue.

Background staining of a preimmune rabbit serum (green) was assessd in the CA3 region of the adult rat brain. Tubulin is depicted in blue. Synapsin is in red.<br />

Background staining of a preimmune rabbit serum (green) was assessd in the somatodendritic compartment of the adult rat hippocampus (CA1 region). Tubulin is depicted in blue. Synapsin is in red.<br />

Smaug 1 knockdown affects synapse size and number. Two out of four sequences against rat Samug 1 (siSmaug1c and siSmaug1 d) elicit the effect. The treatment with siSmaug1a (no effect) is shown. Synapsin is shown in green, and tubulin BIII is in blue.

bioimagesFigure 3 - DFigure 8 - CImage 131266 (Figure 2 - A)Image 131282 (Figure 3 - D)Image 131300 (Figure 9 - C)Image 131298 (Figure 9 - C)Image 131272 (Figure 2 - B)Image 131279 (Figure 3 - D)Image 131270 (Figure 2 - B)Image 131295 (Figure 8 - C)Image 131296 (Figure 9 - C)Figure 9 - CFigure 9Figure 8Image 131267 (Figure 2 - A)Image 131280 (Figure 3 - D)Image 131271 (Figure 2 - B)Image 131281 (Figure 3 - D)Image 131299 (Figure 9 - C)Image 131297 (Figure 9 - C)Figure 2 - AFigure 3Figure 2Figure 2 - BGraciela Lidia BoccaccioMaría Verónica BaezLuciana LuchelliMalena PascualDarío MaschiMartín HabifMaría Gabriela ThomasfalseSmaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation2011-12-26T11:22:28Z2018-11-29T11:22:28Z2018-11-29T11:22:28ZS-JCBD-20110815910.1083/jcb.201108159