{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Javier Fernandez-Martinez"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201109008"],"attach_to":["JCB"],"legend":["Localization of a Nup49p-CFP reporter in the presence of the indicated Nup variant  as determined by fluorescence microscopy. Cells were visualized at room temperature with a 63x, 1.4 numerical aperture plan-apochromat objective (Zeiss) using a Carl Zeiss Axioplan 2 microscope equipped with a Hamamatsu Orca ER-cooled CCD camera. The system was controlled with Openlab imaging software (Perkin Elmer). Final images were assembled, and gamma levels adjusted to enhance contrast only, using Adobe Photoshop software.","Videos were generated with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from the National Institutes of Health (National Center for Research Resources grant 2P41RR001081, National Institute of General Medical Sciences grant 9P41GM103311). “UCSF Chimera--a visualization system for exploratory research and analysis”. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE. J Comput Chem. 2004 Oct;25(13):1605-12.","Time course localization of a Nup49-CFP fluorescent reporter in the presence of Nup133(1-898) at the indicated time after treatment with 0.4% benzyl-alcohol. Cells were visualized by fluorescence microscopy at room temperature with a 63x, 1.4 numerical aperture plan-apochromat objective (Zeiss) using a Carl Zeiss Axioplan 2 microscope equipped with a Hamamatsu Orca ER-cooled CCD camera. The system was controlled with Openlab imaging software (Perkin Elmer). Final images were assembled, and gamma levels adjusted to enhance contrast only, using Adobe Photoshop software.","Localization of a Nup49p-CFP reporter in the presence of the indicated Nup variant as determined by fluorescence microscopy. Cells were visualized at room temperature with a 63x, 1.4 numerical aperture plan-apochromat objective (Zeiss) using a Carl Zeiss Axioplan 2 microscope equipped with a Hamamatsu Orca ER-cooled CCD camera. The system was controlled with Openlab imaging software (Perkin Elmer). Final images were assembled, and gamma levels adjusted to enhance contrast only, using Adobe Photoshop software.","Time course localization of a Nup49-CFP fluorescent reporter in the presence of wild-type Nup133 at the indicated time after treatment with 0.4% benzyl-alcohol. Cells were visualized by fluorescence microscopy at room temperature with a 63x, 1.4 numerical aperture plan-apochromat objective (Zeiss) using a Carl Zeiss Axioplan 2 microscope equipped with a Hamamatsu Orca ER-cooled CCD camera. The system was controlled with Openlab imaging software (Perkin Elmer). Final images were assembled, and gamma levels adjusted to enhance contrast only, using Adobe Photoshop software."],"repository":["bioimages"],"figure_sub":["Figure 8 - Nup85 (wildtype)","Image 135913 (Figure 8 - Nup84(1-573))","Image 135916 (Figure 8 - Nup85(233-744))","Image 135909 (Figure 8 - Nup145c(1-670))","Figure 8 - Nup133(531-1157)","Image 135887 (Figure 8 - Nup133(899-1137))","Image 135900 (Figure 8 - Nup145c(1-468))","Image 135922 (Figure 9 - C)","Figure 8 - Nup145c(1-316)(327-712)","Image 135872 (Figure 8 - Nup120(1-972))","Figure 8 - Nup145c(1-468)","Image 135915 (Figure 8 - Nup84 (wildtype))","Image 135911 (Figure 8 - Nup84(160-726))","Figure 8 - B: delta-NUP133","Image 135921 (Figure 8 - Nup85 (wildtype))","Image 135877 (Figure 8 - Nup133(1-530))","Image 135890 (Figure 8 - Nup133(301-1157))","Image 135907 (Figure 8 - Nup145c(1-641))","Image 135918 (Figure 8 - Nup85(1-438))","Image 135904 (Figure 8 - Nup145c(1-609))","Image 136282 (Video 1 - Rotating views of single structures from the Nup84 complex ensemble)","Image 135882 (Figure 8 - B: delta-NUP133)","Image 135901 (Figure 8 - Nup145c(1-468))","Figure 8 - Nup84(1-573)","Figure 8 - Nup133(1-898)","Image 135879 (Figure 8 - Nup133(531-1157))","Image 135926 (Figure 9 - C)","Figure 8 - Nup85(1-438)","Image 135919 (Figure 8 - Nup85(1-438))","Image 135917 (Figure 8 - Nup85(233-744))","Figure 9","Figure 8","Image 135906 (Figure 8 - Nup145c(1-641))","Figure 8 - Nup84 (wildtype)","Figure 8 - Nup85(233-744)","Image 135884 (Figure 8 - Nup133(1-898))","Image 135908 (Figure 8 - Nup145c(1-670))","Image 135873 (Figure 8 - Nup120(1-972))","Figure 8 - Nup84(160-726)","Image 135910 (Figure 8 - Nup84(160-726))","Figure 8 - Nup145c(1-227)","Image 135924 (Figure 9 - C)","Image 136280 (Video 1 - Rotating views of single structures from the Nup84 complex ensemble)","Image 136278 (Video 1 - Rotating views of single structures from the Nup84 complex ensemble)","Image 135903 (Figure 8 - Nup145c (wildtype))","Image 136279 (Video 1 - Rotating views of single structures from the Nup84 complex ensemble)","Figure 8 - Nup133(301-1157)","Image 135878 (Figure 8 - Nup133(531-1157))","Image 135912 (Figure 8 - Nup84(1-573))","Image 135892 (Figure 8 - Nup133 (wildtype))","Image 135927 (Figure 9 - C)","Figure 9 - C","Figure 8 - Nup133(899-1137)","Image 135889 (Figure 8 - Nup133(1-1086))","Figure 8 - Nup145c(1-670)","Video 1 - Rotating views of single structures from the Nup84 complex ensemble","Figure 8 - Nup120(1-817)","Video 1","Image 135914 (Figure 8 - Nup84 (wildtype))","Image 135871 (Figure 8 - Nup120(1-817))","Figure 8 - Nup133 (wildtype)","Image 135885 (Figure 8 - Nup133(1-898))","Image 135886 (Figure 8 - Nup133(899-1137))","Image 135888 (Figure 8 - Nup133(1-1086))","Figure 8 - Nup120 (wildtype)","Figure 8 - Nup145c(1-609)","Image 135898 (Figure 8 - Nup145c(1-227))","Image 135869 (Figure 8 - Nup120(397-1037))","Figure 8 - Nup120(397-1037)","Figure 8 - Nup133(1-1086)","Image 135891 (Figure 8 - Nup133(301-1157))","Figure 8 - Nup145c(1-641)","Image 135905 (Figure 8 - Nup145c(1-609))","Image 135874 (Figure 8 - Nup120 (wildtype))","Figure 8 - Nup145c (wildtype)","Image 135923 (Figure 9 - C)","Figure 8 - Nup133(1-530)","Figure 8 - Nup133(1-772)","Image 135895 (Figure 8 - Nup145c(1-316)(327-712))","Image 135881 (Figure 8 - Nup133(1-772))","Image 135868 (Figure 8 - Nup120(397-1037))","Image 135896 (Figure 8 - Nup145c(1-128)(181-712))","Figure 8 - Nup120(1-972)","Image 135880 (Figure 8 - Nup133(1-772))","Image 135893 (Figure 8 - Nup133 (wildtype))","Figure 8 - Nup145c(1-128)(181-712)","Image 135897 (Figure 8 - Nup145c(1-128)(181-712))","Image 135870 (Figure 8 - Nup120(1-817))","Image 135883 (Figure 8 - B: delta-NUP133)","Image 135902 (Figure 8 - Nup145c (wildtype))","Image 135876 (Figure 8 - Nup133(1-530))","Image 135920 (Figure 8 - Nup85 (wildtype))","Image 135875 (Figure 8 - Nup120 (wildtype))","Image 136277 (Video 1 - Rotating views of single structures from the Nup84 complex ensemble)","Image 135899 (Figure 8 - Nup145c(1-227))","Image 135925 (Figure 9 - C)","Image 135894 (Figure 8 - Nup145c(1-316)(327-712))"],"pubmed_authors":["Matthew D. Sekedat","Josef D. Franke","David L. Stokes","Rosemary Williams","Jeremy Phillips","Andrej Sali","Javier Fernandez-Martinez","Ruben Diaz-Avalos","Javier Velazquez-Muriel","Michael P. Rout","Brian T. Chait"],"additional_accession":[]},"is_claimable":false,"name":"Structure–function mapping of a heptameric module in the nuclear pore complex","description":null,"dates":{"release":"2012-02-13T11:22:30Z","modification":"2018-11-29T11:22:30Z","creation":"2018-11-29T11:22:30Z"},"accession":"S-JCBD-201109008","cross_references":{"doi":["10.1083/jcb.201109008"]}}