{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Emanuele Roscioli"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201109104"],"attach_to":["JCB"],"legend":["RANGAP1 localization in a metaphase cell transfected with importin-beta 1-876 (full-length) and with H2B-GFP to visualize chromosomes in the transfected cell. RANGAP1 is stained in red, alpha tubulin in blue and H2B in green. Note that the shape of the metaphase spindle is comparable to that of the control cell, yet RANGAP1 accumulation at attached kinetochores is severely impaired. Shown is a single stack image taken with an Olympus AX70 epifluorescence microscope (100x objective) equipped with IAS 2000 software (Delta Sistemi).","Shown is a metaphase cell co-transfected with importin-beta 1-876, together with the middle BPM fragment from RANBP2  and with H2B-GFP as a transfection marker. The spindle (alpha-tubulin) is stained in red, H2B in green and chromosomes in blue (DAPI). Note that chromosomes are correctly aligned in the co-transfected cell. Shown is the maximum intensity projection (MaxIP) of 7 stack images (0.6 micrometers) acquired under a Nikon 90i epifluorescence microscope (100x objective, 1.3 NA) equipped with the Nis-elements 3.1 software (Nikon).","Shown is a metaphase cell co-transfected with importin-beta 1-876, together with the N-terminal BPN fragment from RANBP2 and H2B-GFP as a transfection marker. The spindle (alpha-tubulin) is stained in red, H2B in green and chromosomes in blue (DAPI). Note the misaligned chromosomes. Shown is the maximum intensity projection (MaxIP) of 10 stack images (0.8 micrometers) taken under a Nikon 90i epifluorescence microscope (100x objective, 1.3 NA) equipped with the Nis-elements 3.1 software (Nikon).","A metaphase cell expressing importin-beta 45-462 (deleted in both the N- and C-termini) and H2B-GFP to visualize chromosomes. RANGAP1 is shown in red, alpha tubulin in blue and H2B in green. Note that RANGAP1 fails to be recruited onto microtubule-attached kinetochores in the metaphase on the left side of the field. The heavily H2B-GFP-transfected cell on the right side is in prometaphase. Shown is a single stack image acquired under an Olympus AX70 epifluorescence microscope (100x objective) equipped with IAS 2000 software (Delta Sistemi).","RANGAP1 localization in a control metaphase cell transfected with empty vector and H2B-GFP to visualize chromosomes (in the center of the field). RANGAP1 is labeled in red, alpha-tubulin in blue and H2B in green. Note the localization of RANGAP1 at microtubule-attached kinetochores and along the spindle microtubules. Shown is a single stack image taken  under an Olympus AX70 epifluorescence microscope (100x objective) equipped with the IAS 2000 software (Delta Sistemi). In order to appreciate the punctuate RANGAP1 signals at kinetochores, the surrounding interphase cells appear overexposed.","Shown is a metaphase cell co-transfected with importin beta 1-876 (full-length) and H2B-GFP to visualize chromosomes. The spindle (alpha-tubulin) is stained in red, H2B in green and chromosomes in blue (DAPI). Misaligned chromosomes are clearly visible near the upper pole. We show the maximum intensity projection (MaxIP) of 7 stack images (0.6 micrometers) acquired under a Nikon 90i epifluorescence microscope (100x objective, 1.3 NA) equipped with the Nis-elements 3.1 software (Nikon)."],"repository":["bioimages"],"figure_sub":["Image 30630 (Figure 8 - B)","Figure 7","Image 30380 (Figure 7 - A)","Image 30631 (Figure 8 - B)","Figure 8","Figure 8 - B","Image 30377 (Figure 7 - A)","Figure 7 - A","Image 30632 (Figure 8 - B)","Image 30383 (Figure 7 - A)"],"pubmed_authors":["Maria Giubettini","Patrizia Lavia","Emanuele Roscioli","Laura Di Francesco","Alessio Bolognesi","Amnon Harel","Maria Eugenia Schininà","Serena Orlando"],"additional_accession":[]},"is_claimable":false,"name":"Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores","description":null,"dates":{"release":"2012-02-13T11:22:33Z","modification":"2018-11-29T11:22:33Z","creation":"2018-11-29T11:22:33Z"},"accession":"S-JCBD-201109104","cross_references":{"doi":["10.1083/jcb.201109104"]}}