bioimagesUnknownSophie LutterThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201110132JCB

Confocal z-stack of wild-type mouse ear skin at P14 stained with antibodies against LYVE-1 (green), aSMA (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse mesentery at postnatal day 16 stained with antibodies against Reelin (green), aSMA (red) and PECAM-1 (blue).

Montage of confocal images (maximum intensity projections of z-stacks) showing a dermal collecting lymphatic vessel in the ear of a 3-week old Apoer2;Vldlr double mutant mouse from proximal (right) to distal (left) end. The tissue was stained with antibodies against LYVE-1 (green), aSMA (red) and podoplanin (blue).

Confocal z-stack of wild-type mouse ear skin at P16 stained with antibodies against LYVE-1 (green), desmin (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P12 stained with antibodies against LYVE-1 (green), desmin (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P21 showing uptake of intradermally injected FITC-dextran (green) into dermal lymphatic vessels, stained with antibodies against LYVE-1 (red) and aSMA (blue).

Confocal z-stack of wild-type mouse ear skin at P16 stained with antibodies against Laminin-a5 (green), aSMA (red) and LYVE-1 (blue).

Confocal z-stack of Reln mutant mouse collecting vessel stained with antibodies against LYVE-1 (green), aSMA (red) and podoplanin (blue).

Confocal z-stack of Reln mutant mouse collecting vessel stained with antibodies against Integrin-a9 (green), aSMA (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P21 stained with antibodies against NG2 (green), aSMA (red) and podoplanin (blue).

Montage of confocal images showing a dermal collecting lymphatic vessel in the P20 ear of a wild-type mouse from proximal (right) to distal (left) end. The tissue was stained with antibodies against LYVE-1 (blue), aSMA (red) and podoplanin (green).

Confocal z-stack of wild-type mouse skin at postnatal day 21 stained with antibodies against Reelin (green) and PECAM-1 (red). The tissue was not permeabilised, and Reelin staining was amplified using the TSA system.

Montage of confocal images (maximum intensity projections of z-stacks) showing a dermal collecting lymphatic vessel in the ear of a 3-week old Dab1 mutant mouse from proximal (right) to distal (left) end. The tissue was stained with antibodies against LYVE-1 (green), aSMA (red) and podoplanin (blue).

Confocal z-stack of wild-type mouse skin at embryonic day 16 stained with antibodies against Reelin (green), LYVE-1 (red) and PECAM-1 (blue).

Western blot analysis of PECAM-1 expression. The left three lanes represent the samples that were analysed by immunoprecipitation with anti-Reln antibody in the other panels of this figure part.

Confocal z-stack of wild-type mouse ear skin at P12 stained with antibodies against LYVE-1 (green), aSMA (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P14 stained with antibodies against Laminin-a5 (green), aSMA (red) and LYVE-1 (blue).

Confocal z-stack of wild-type mouse mesentery at postnatal day 16 stained with antibodies against Reelin (green), aSMA (red) and PECAM-1 (blue).

Left three lanes: analysis of cell lysates<br /><br />Right three lanes: analysis of culture supernatant

Confocal z-stack of wild-type mouse ear skin at P14 stained with antibodies against LYVE-1 (green), desmin (red) and PECAM-1 (blue).

Confocal z-stack of Reelin mutant mouse ear skin at P21 showing uptake of intradermally injected FITC-dextran (green) into dermal lymphatic vessels, stained with antibodies against LYVE-1 (red).

Montage of confocal images showing a dermal collecting lymphatic vessel in the P20 ear of a Reln mutant mouse from proximal (right) to distal (left) end. The tissue was stained with antibodies against LYVE-1 (blue), aSMA (red) and podoplanin (green).

Confocal z-stack of wild-type mouse ear skin at P16 stained with antibodies against LYVE-1 (green), aSMA (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse collecting vessel stained with antibodies against Integrin-a9 (green), aSMA (red) and PECAM-1 (blue).

Montage of confocal images (maximum intensity projections of z-stacks) showing dermal collecting lymphatic vessel in the ear of a 3-week old wild-type mouse from proximal (right) to distal (left) end. The tissue was stained with antibodies against LYVE-1 (green), aSMA (red) and podoplanin (blue).

Immunoprecipitation with anti-Reelin antibody and Western blot analysis of lymphatic endothelial cell-conditioned medium with (left lane) or without (second lane from left) incubation with smooth muscle cells.

Confocal z-stack of Reelin mutant mouse ear skin at P21 showing uptake of intradermally injected FITC-dextran (green) into dermal lymphatic vessels, stained with antibodies against aSMA (red) and LYVE-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P21 stained with antibodies against desmin (green), aSMA (red) and podoplanin (blue).

Western blot analysis of aSMA expression. The left three lanes represent the samples that were analysed by immunoprecipitation with anti-Reln antibody in the other panels of this figure part.

Confocal z-stack of wild-type mouse ear skin at P21 showing uptake of intradermally injected FITC-dextran (green) into dermal lymphatic vessels, stained with antibodies against aSMA (red) and LYVE-1 (blue).

Confocal z-stack of wild-type mouse collecting vessel stained with antibodies against LYVE-1 (green), aSMA (red) and podoplanin (blue).

Confocal z-stack of wild-type mouse ear skin at P14 stained with antibodies against collagen IV (green), aSMA (red) and LYVE-1 (blue).

Confocal z-stack of wild-type mouse skin at postnatal day 21 stained with antibodies against Reelin (green), aSMA (red) and PECAM-1 (blue).

Confocal z-stack of wild-type mouse ear skin at P16 stained with antibodies against collagen IV (green), aSMA (red) and LYVE-1 (blue).

Confocal z-stack of wild-type mouse collecting vessel stained with antibodies against LYVE-1 (green), aSMA (red) and PECAM-1 (blue).

bioimagesImage 136084 (Figure 4 - F)Image 136052 (Figure 1 - I)Image 136096 (Figure 2 - E)Image 136091 (Figure 3 - F)Supplemental Figure 2Image 136048 (Figure 1 - E)Image 136050 (Figure 1 - F)Image 136095 (Figure 2 - D)Supplemental Figure 2 - ESupplemental Figure 2 - FImage 136051 (Figure 1 - H)Image 136047 (Figure 1 - D)Image 136049 (Figure 1 - G)Image 136046 (Figure 1 - C)Figure 1 - IFigure 1 - HFigure 1 - GImage 136077 (Figure 5 - A)Figure 1 - FFigure 1 - LFigure 1 - KFigure 1 - JFigure 1 - AImage 136097 (Figure 2 - F)Image 136079 (Figure 5 - B)Figure 1 - EFigure 1 - DImage 136090 (Figure 3 - C: aSMA)Figure 1 - CFigure 1 - BFigure 2 - GImage 136059 (Figure 6 - E)Figure 2 - EFigure 2 - FImage 136083 (Figure 4 - D)Image 136080 (Figure 5 - D)Image 136045 (Figure 1 - B)Figure 2 - CImage 136089 (Figure 3 - C: CD31)Figure 2 - DFigure 2 - AFigure 2 - BFigure 3 - FImage 136087 (Figure 4 - I)Image 136055 (Figure 1 - L)Image 136044 (Figure 1 - A)Image 136093 (Figure 2 - B)Image 136098 (Figure 2 - G)Figure 3 - C: aSMAFigure 3 - C: CD31Figure 3 - C,D: RelnFigure 4 - IFigure 4 - GFigure 4 - HImage 136058 (Figure 6 - D)Figure 4 - FFigure 4 - DImage 136276 (Supplemental Figure 2 - F)Figure 5 - AImage 136094 (Figure 2 - C)Image 136088 (Figure 3 - C,D: Reln)Image 136057 (Figure 6 - C)Image 136086 (Figure 4 - H)Image 136054 (Figure 1 - K)Figure 5 - BFigure 5 - CFigure 5 - DFigure 5Image 136275 (Supplemental Figure 2 - E)Figure 4Figure 6Image 136092 (Figure 2 - A)Figure 6 - CFigure 6 - DImage 136085 (Figure 4 - G)Image 136053 (Figure 1 - J)Image 136078 (Figure 5 - C)Figure 1Figure 3Figure 6 - EFigure 2Sherry XieTaija MakinenSophie LutterFlorence TatinfalseSmooth muscle–endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation2012-06-04T11:22:38Z2018-11-29T11:22:38Z2018-11-29T11:22:38ZS-JCBD-20111013210.1083/jcb.201110132