{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Tsukasa Oikawa"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201111116"],"attach_to":["JCB"],"legend":["RAW264.7 cells expressing the reporter construct RFP-2xPLCδ1PH-IRES-GFP-AktPH (in red and green) in the presence of Dox (1 µg/ml) were transfected on two consecutive days with Tks5 siRNA and then stimulated with RANKL (10 ng/ml) for 48 h. Cells were exposed to DAPI at 1 µg/ml (blue) for 1 h before observation to visualize nuclei. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (Fluoview FV10i; Olympus). Frames were taken every 3 min. Each time point is a series of 4 focal planes separated by 1 µm for each color.","RAW264.7 cells expressing the reporter construct RFP-2xPLCδ1PH-IRES-GFP-AktPH (in red and green) in the presence of Dox (1 µg/ml) were transfected on two consecutive days with control (Ctr) siRNA and then stimulated with RANKL (10 ng/ml) for 48 h. Cells were exposed to DAPI at 1 µg/ml (blue) for 1 h before observation to visualize nuclei. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (Fluoview FV10i; Olympus). Frames were taken every 3 min. Each time point is a series of 4 focal planes separated by 1 µm for each color."],"repository":["bioimages"],"figure_sub":["Image 134235 (Video 7 - None)","Image 134236 (Video 7 - None)","Video 7","Video 7 - None"],"pubmed_authors":["Hideyuki Saya","Nobuyuki Udagawa","Masaaki Oyama","Shunsuke Uehara","Tsukasa Oikawa","Koichi Matsuo","Hiroko Kozuka-Hata"],"additional_accession":[]},"is_claimable":false,"name":"Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell–cell fusion","description":null,"dates":{"release":"2012-05-14T11:22:45Z","modification":"2018-11-29T11:22:45Z","creation":"2018-11-29T11:22:45Z"},"accession":"S-JCBD-201111116","cross_references":{"doi":["10.1083/jcb.201111116"]}}