bioimagesUnknownTsukasa OikawaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201111116JCB

RAW264.7 cells expressing the reporter construct RFP-2xPLCδ1PH-IRES-GFP-AktPH (in red and green) in the presence of Dox (1 µg/ml) were transfected on two consecutive days with Tks5 siRNA and then stimulated with RANKL (10 ng/ml) for 48 h. Cells were exposed to DAPI at 1 µg/ml (blue) for 1 h before observation to visualize nuclei. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (Fluoview FV10i; Olympus). Frames were taken every 3 min. Each time point is a series of 4 focal planes separated by 1 µm for each color.

RAW264.7 cells expressing the reporter construct RFP-2xPLCδ1PH-IRES-GFP-AktPH (in red and green) in the presence of Dox (1 µg/ml) were transfected on two consecutive days with control (Ctr) siRNA and then stimulated with RANKL (10 ng/ml) for 48 h. Cells were exposed to DAPI at 1 µg/ml (blue) for 1 h before observation to visualize nuclei. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (Fluoview FV10i; Olympus). Frames were taken every 3 min. Each time point is a series of 4 focal planes separated by 1 µm for each color.

bioimagesImage 134235 (Video 7 - None)Image 134236 (Video 7 - None)Video 7Video 7 - NoneHideyuki SayaNobuyuki UdagawaMasaaki OyamaShunsuke UeharaTsukasa OikawaKoichi MatsuoHiroko Kozuka-HatafalseTks5-dependent formation of circumferential podosomes/invadopodia mediates cell–cell fusion2012-05-14T11:22:45Z2018-11-29T11:22:45Z2018-11-29T11:22:45ZS-JCBD-20111111610.1083/jcb.201111116