{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Nadine Schmidt"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201111130"],"attach_to":["JCB"],"legend":["Total image stack of CLASP2-deficient NMJ stained for AChRs (red) and GFP-CLIP-170 (green), acquired by structural illumination microscopy. Note lower rate of AChR/GFP-CLIP-170 colocalization compared to wild-type NMJ (B1), consistent with lower rate of microtubule capturing at synaptic membrane in the absence of CLASP2.","At wild-type NMJs, CLASP2 (green) is also enhanced at the edge of synaptic AChR cluster (red).","Confocal image of CLASP2 staining (green) taken deeper within the synaptic gutter of a wild-type NMJ suggests that CLASP2 like CLIP-170 localizes to the crests of the primary folds (AChR, red).","Greyscale image of nuclei only in wild-type muscle. Schwann cell nuclei are marked by asterisks, whereas subsynaptic myonuclei are marked by arrowheads.","Total image stack of wild-type NMJ stained for AChRs (red) and GFP-CLIP-170 (green), acquired by structural illumination microscopy. The red staining in individual optical slices mark AChRs at the same respective z-levels of synaptic membrane (compare contour line in Fig4B). Note the frequent co-localization of GFP-CLIP-170 with AChR contour lines in wild-type synaptic membrane.","In CLASP-deficient muscle, CLASP2 staining (green) with the antibody used in wild-type before (C1) is absent from the NMJ (AChR, red).","Greyscale image of the nuclei only in CLASP2-deficient muscle.  Schwann cell nuclei are marked by asterisks and subsynaptic myonuclei are marked by arrowheads.  Absence of CLASP2 results in a decrease of subsynaptic muscle nuclei underlying the AChR cluster.","Saturating labeling of AChRs with BTX-Alexa594 reveals that the synaptic AChR density is reduced in CLASP2-deficient NMJs.","Saturating labeling of AChRs with BTX-Alexa594 in wild-type muscle in vivo.","Saturating labeling of AChRs with BTX-Alexa594 in CLIP115;CLIP170-deficient muscle in vivo. Deficiency in CLIP115 and CLIP170 decreases the AChR density. <br />","Video: The PI3-kinase inhibitor ZSTK474 impairs capturing of GFP-CLIP-170 (greyscale) comets at agrin-induced AChR clusters. Treatment of GFP-Clip-170ki/ki myotube with ZSTK474 abolishes the difference in MT dynamics within and outside the agrin-induced AChR cluster (marked in Fig. 6D), as does deletion of CLASP2 (see Suppl. Movie S2). Frames are acquired over a period of 160 s (1 frame/s) in the TIRF plane<br />(inverted Nikon TE2000 TIRF microscope equipped with an oil immersion HCX PL<br />APO 100x TIRF objective (NA=1.49)).<br />","CLIP-170 staining at wild-type NMJ reveals pronounced localization of CLIP-170 (green) at the crest of the primary folds as well as on the edge of synaptic AChR cluster (red).","Confocal image taken at the level of the edge of a wild-type NMJ stained for CLIP-170/ CLASP2/ AChRs. Note the co-localization and enrichment of CLIP-170 (green) and CLASP2 (red) along the edge of the synaptic AChR cluster (blue).","Confocal image of a wild-type NMJ stained for AChRs (red), Schwann cells (green) and nuclei (blue).The number of subsynaptic muscle nuclei is determined by subtracting the number of Schwann cell nuclei (revealed by sourrounding Schwann cell staining) from the number of nuclei underlying the AChR cluster.","Saturating labeling of AChRs with BTX-Alexa488 (not shown) in superficial CLASP2-deficient endplates in vivo was followed 7 days later by staining of newly inserted AChRs with BTX-Alexa594. Deletion of CLASP2 reduces the insertion rate of newly synthesized AChRs into the synaptic membrane.","Confocal image of a CLASP2-deficient NMJ stained for AChRs (red), Schwann cells (green) and nuclei (blue).","Immunolabeling of EB3 (red), GFP-CLIP170 (green) and AChRs (blue)  in a GFP-Clip-170ki/ki myotube. In the agrin-induced AChR cluster the load of CLIP-170 is increased compared to outside of the cluster, whereas EB3 staining is similar in both regions.","Saturating labeling of AChRs with BTX-Alexa488 (not shown) in superficial wild-type endplates in vivo  was followed 7 days later by staining of newly inserted AChRs with BTX-Alexa594. Note that receptor insertion is most prominent at the edge of the synaptic AChR cluster.","Immunolabeling of EB3 (red), GFP-CLIP170 (green) and AChRs (blue) in a GFP-Clip-170ki/ki myotube after incubation with the PI3Kinase blocker ZSTK474. Note the reduced GFP-CLIP-170 load at the AChR cluster upon inhibition of PI3Kinase activity."],"repository":["bioimages"],"figure_sub":["Image 136733 (Figure 7 - A)","Image 136723 (Figure 3 - A)","Image 136731 (Figure 4 - B)","Image 136739 (Figure 3 - B)","Image 136719 (Figure 2 - C)","Figure 7 - A","Figure 7 - B","Figure 3 - A","Figure 3 - B","Figure 3 - C","Image 136717 (Figure 2 - A)","Image 136732 (Figure 4 - B)","Figure 4 - B","Image 136741 (Figure 3 - C)","Image 136738 (Figure 3 - B)","Image 136724 (Figure 3 - A)","Image 136736 (Figure 3 - B)","Image 136721 (Figure 3 - A)","Image 136737 (Figure 3 - B)","Image 136734 (Figure 7 - B)","Image 136742 (Figure 3 - C)","Image 136722 (Figure 3 - A)","Figure 4","Figure 7","Figure 6","Image 136744 (Figure 6 - D)","Image 136720 (Figure 2 - C)","Figure 6 - D","Image 136718 (Figure 2 - B)","Figure 2 - C","Image 136716 (Figure 2 - A)","Figure 2 - A","Figure 3","Figure 2","Figure 2 - B"],"pubmed_authors":["Sabrina Gatti","Jeffrey van Haren","Nadine Schmidt","Hans Rudolf Brenner","Stefan Sladecek","Jan Pielage","Sreya Basu","Niels Galjart","Susan Treves"],"additional_accession":[]},"is_claimable":false,"name":"Agrin regulates CLASP2-mediated capture of microtubules at the neuromuscular junction synaptic membrane","description":null,"dates":{"release":"2012-07-30T11:22:46Z","modification":"2018-11-29T11:22:46Z","creation":"2018-11-29T11:22:46Z"},"accession":"S-JCBD-201111130","cross_references":{"doi":["10.1083/jcb.201111130"]}}