bioimagesUnknownXianying TangThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201112017JCB

Full 3D stack of confocal images of Cox4-RFP in a CC(1-303)-PH yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Uncropped field showing reconstituted venus fluorescence in yeast cells expressing VC-Num1, Dyn1-VN, and CFP-Tub1.

Uncropped image of a single focal plane of wild-type yeast expressing CC(1-303)-PH-GFP and detected for GFP fluorescence.

Full 3D stack of confocal images of a calcofluor-stained CC(1-303)-GFP-CAAX yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D to indicate the cell periphery. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

The CC(1-303)-PH mutant is defective in spindle oscillation. Wide-field time-lapse GFP images of hydroxyurea-arrested CC(1-303)-PH kar9∆ yeast cells expressing GFP-Tub1 were acquired every 10 s. Each time point is a series of 5 focal planes separated by 0.5 μm.

Spindle oscillation in a NUM1 cell. Wide-field time-lapse GFP images of hydroxyurea-arrested NUM1 kar9∆ yeast cells expressing GFP-Tub1 were acquired every 10 s. Each time point is a series of 5 focal planes separated by 0.5 μm. <br /><br />

Uncropped bright field image of wild-type yeast expressing VC-CC(1-303), Dyn1-VN, and CFP-Tub1.

Uncropped field showing reconstituted venus fluorescence in yeast cells expressing VC-CC(1-303), Dyn1-VN, and CFP-Tub1.

Full 3D stack of confocal images of Cox4-RFP in a CC(1-303)-GFP-CAAX yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Bright field for CC(1-303)-PH Cox4-RFP dyn1∆ yeast cells.

NUM1-GFP mCherry-TUB1 yeast cells were imaged in the same field with num1(E191A+K192A)-GFP cells. A single focal plane image of mCherry is shown.

NUM1-GFP mCherry-TUB1 yeast cells were imaged in the same field with num1(E191A+K192A)-GFP cells. A single focal plane GFP image is shown.

Full 3D stack of confocal images of a calcofluor-stained ∆CC1 yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D to indicate the cell periphery. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Full 3D stack of confocal images of Cox4-RFP in a num1∆ yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Bright field for CC(1-303)-PH Cox4-RFP nip100∆ yeast cells

Cortical mitochondrial attachment in CC(1-303)-PH nip100∆ yeast cells. Wide-field two-color time-lapse images of Cox4-RFP (blue) and calcofluor (red) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Uncropped field showing CFP-Tub1 in yeast cells expressing VC-CC(1-303), Dyn1-VN, and CFP-Tub1.

Full 3D stack of confocal images of a calcofluor-stained wild-type yeast cell (NUM1) expressing Cox4-RFP. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D to indicate the cell periphery. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

num1(L167E+L170E) yeast cells expressing Dyn1-3GFP and mCherry-Tub1 showing loss of cortical dynein foci. Wide-field two-color time-lapse images of Dyn1-3GFP (red) and mCherry-Tub1 (blue) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Full 3D stack of confocal images of a calcofluor-stained CC(1-303)-PH yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D to indicate the cell periphery. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Cortical mitochondrial attachment in CC(1-303)-PH dyn1∆ yeast cells. Wide-field two-color time-lapse images of Cox4-RFP (blue) and calcofluor (red) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Uncropped image of a single focal plane of yeast cells expressing num1(L167E+L170E)-GFP and detected for GFP fluorescence.

num1(L167E+L170E) yeast cells expressing Jnm1-3mCherry and CFP-Tub1 showing loss of cortical Jnm1-3mCherry foci. Wide-field two-color time-lapse images of Jmm1-3mCherry (red) and CFP-Tub1 (green) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Full 3D stack of confocal images of a calcofluor-stained num1∆ yeast cell expressing Cox4-RFP. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D to indicate the cell periphery. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Uncropped image of a single focal plane of wild-type yeast expressing ∆CC-GFP and detected for GFP fluorescence.

Full 3D stack of confocal images of Cox4-RFP in a ∆CC1 yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Cortical mitochondrial attachment in num1(L167E+L170E) yeast cells expressing Cox4-RFP. Wide-field two-color time-lapse images of Cox4-RFP (red) and calcofluor (blue) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Uncropped field showing CFP-Tub1 in yeast cells expressing VC-Num1, Dyn1-VN, and CFP-Tub1.

Full 3D stack of confocal images of Cox4-RFP in a wild-type NUM1 yeast cell. Equatorial z-slices were projected (totaling 0.8 µm thick) and shown in Figure 5D. (The calcofluor-stained cell periphery is displayed in green and Cox4-RFP in red in Fig. 5D.)

Uncropped image of a single focal plane of wild-type yeast expressing ∆TR-GFP and detected for GFP fluorescence.

Uncropped bright field image of wild-type yeast expressing VC-Num1, Dyn1-VN, and CFP-Tub1.

Cortical mitochondrial attachment in wild-type yeast cells expressing Cox4-RFP. Wide-field two-color time-lapse images of Cox4-RFP (blue) and calcofluor (red) were acquired every 5 s. Each time point is a series of 3 focal planes separated by 1 μm for each color.

Uncropped image of a single focal plane of wild-type yeast expressing Num1-GFP and detected for GFP fluorescence.

Uncropped image of a single focal plane of wild-type yeast expressing ∆CC1-GFP and detected for GFP fluorescence.

bioimagesFigure 8 - CImage 33459 (Figure 5 - D)Figure 8 - BVideo 7Video 7 - NoneFigure 7 - AImage 133236 (Figure 8 - C)Image 133242 (Figure 3 - F)Image 33463 (Figure 5 - D)Image 33372 (Figure 4 - B - VC-CC(1-303))Image 131332 (Figure 7 - A)Image 33376 (Figure 4 - B - VC-Num1)Image 133238 (Video 7 - None)Image 33375 (Figure 4 - B - VC-Num1)Image 33373 (Figure 4 - B - VC-CC(1-303))Image 33379 (Figure 4 - B - VC-Num1)Image 33378 (Figure 4 - B - VC-Num1)Image 133152 (Figure 5 - E)Image 133241 (Figure 3 - F)Image 33377 (Figure 4 - B - VC-Num1)Image 33464 (Figure 5 - D)Figure 2 - AImage 133134 (Figure 8 - B)Figure 3 - FImage 131331 (Figure 7 - A)Image 33374 (Figure 4 - B - VC-CC(1-303))Figure 4 - B - VC-CC(1-303)Image 33458 (Figure 5 - D)Image 133237 (Video 7 - None)Image 33462 (Figure 5 - D)Image 133235 (Figure 8 - C)Image 33370 (Figure 2 - A)Image 133233 (Figure 5 - E)Image 33368 (Figure 2 - A)Image 33457 (Figure 5 - D)Image 133155 (Figure 5 - E)Image 33369 (Figure 2 - A)Image 133234 (Figure 5 - E)Image 33371 (Figure 2 - A)Figure 5 - DImage 33461 (Figure 5 - D)Figure 5 - EFigure 5Figure 4Image 33380 (Figure 4 - B - VC-Num1)Figure 7Image 33367 (Figure 2 - A)Figure 8Image 33456 (Figure 5 - D)Image 33455 (Figure 5 - D)Figure 4 - B - VC-Num1Figure 3Image 33460 (Figure 5 - D)Figure 2Xianying TangBryan St. GermainWei-Lih LeefalseA novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning2012-03-19T11:22:48Z2018-11-29T11:22:48Z2018-11-29T11:22:48ZS-JCBD-20111201710.1083/jcb.201112017