bioimagesUnknownP. Taylor EvesThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201201024JCB

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1563 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 780 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 448 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 196 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 660 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2100 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 720 s<br />

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 896 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1416 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 550 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1002 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1212 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers.Red, vacuoles; green, mitochondria; grey, DIC. 420 s<br />

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 375 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1800 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 440 s<br />

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 522 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1300 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 640 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1392 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 644 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 448 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2262 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1404 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 84 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 432 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1296 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1450 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 630 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 196 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1500 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 160 s<br />

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 850 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1560 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1150 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1000 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 644 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1017 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1100 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1204 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 780 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 440 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 650 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 280 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 400 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 348 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s <br />

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1450 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s, scale bar 5um

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 216 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2208 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1008 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1650 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1250 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 896 s

Figure 4, V. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 630 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s<br />

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 393 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 744 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 980 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 392 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 420 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1812 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1368 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 40 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1524 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1100 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 174 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 280 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 315 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1900 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 280 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 84 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1719 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 680 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 754 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 160 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 276 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 560 s<br />

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 660 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 990 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 690 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1160 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1620 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 336 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 975 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 420 s

Figure 4, V. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 324 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 840 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 950 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 280 s<br />

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1350 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 510 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 666 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 650 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 50 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 520 s<br />

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1890 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2310 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 216 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 400 s<br />

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1018 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 432 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1188 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1550 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 660 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 240 s<br />

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1550 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1218 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 392 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 50 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1350 s

Figure 4, V. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 840 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1914 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1890 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 270 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 840 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2150 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1794 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2000 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1797 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1710 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 650 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 696 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 504 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 260 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2000 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 350 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 640 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1980 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 720 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1566 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 20 s<br />

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 714 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1950 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 276 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 90 s<br />

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 420 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria, grey, DIC. 0 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 471 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1150 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 39 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1446 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 440 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1080 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2940 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 308 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2031 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1450 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 40 s<br />

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 580 s<br />

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1440 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 582 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 294 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 756 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 414 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 120 s<br />

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1400 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1250 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 140 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 90 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria, grey, DIC. 1000 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1700 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1992 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 570 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 950 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 560 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2100 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1350 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 120 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1900 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1650 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1950 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 870 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s<br />

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 426 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 26 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 660 s<br />

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2210 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 252 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 948 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 627 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1758 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2520 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1290 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 320 s<br />

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 549 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 438 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1850 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 680 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1362 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 380 s<br />

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1300 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 180 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 980 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1798 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 186 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1334 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 978 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1308 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 50 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1064 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 28 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1316 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 336 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 290 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 220 s<br />

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1095 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1104 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1125 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 690 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 868 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 480 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 784 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 532 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1740 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 174 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 306 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1680 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 516 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 75 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 20 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 224 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 928 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1150 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2730 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 240 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 116 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 198 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1260 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 760 s<br />

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 828 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 504 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 58 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 318 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 825 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1800 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 13 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 550 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 588 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 822 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1641 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 492 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2460 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 60 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1518s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1152 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 720 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1308 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 400 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2358 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 140 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1350 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1600 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 460 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 850 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1080 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 198 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1750 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 372 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1470 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1040 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1550 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1288 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 260 s<br />

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 756 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 232 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 560 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 240 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1682 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 810 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 552 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 159 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 250 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s, scale bar 5um<br />

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 120 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 138 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 705 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 384 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 812 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1329 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 580 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 820 s<br />

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1092 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 354 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1458 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 112 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 840 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 812 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 340 s<br />

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 364 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 396 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1700 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 120 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1080 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 340 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1500 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1875 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1251 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 732 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 920 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1400 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 264 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 616 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 648 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 850 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 675 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 812 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1102 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 80 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 476 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2050 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1000 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1400 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1250 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1260 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2310 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1098 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 744 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 864 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 252 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s, scale bar 5um

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1656 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 112 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 720 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 56 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 924 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 140 s<br />

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 728 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 476 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1750 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 740 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 220 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1950 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1450 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1372 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2100 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 464 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 650 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 168 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1512 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1380 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 924 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 144 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 728 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 132 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1150 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1280 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 648 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 672 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 276 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 360 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1600 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 616 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1176 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1953 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 480 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 350 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1260 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 460 s<br />

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1908 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 558 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2050 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1962 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1400 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2112 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 66 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 364 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 168 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1300 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1120 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 180 s<br />

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1508 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 546 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1524 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 156 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 480 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2100 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 620 s<br />

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 972 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 78 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 560 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1100 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1500 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1173 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1160 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1050 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 462 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 840 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 534 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 550 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 180 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1850 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 250 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 952 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 400 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 320 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 330 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1092 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 308 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 525 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 588 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 986 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1276 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1260 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1680 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 108 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1000 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1650 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 756 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s<br />

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 342 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1242 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 252 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 100 s<br />

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1500 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1662 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 504 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 756 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 550 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 750 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1148 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1856 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1250 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 600 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 56 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 380 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1740 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1134 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1344 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1850 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1900 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 60 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 237 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 250 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1600 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1620 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2058 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 966 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1485 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1608 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 222 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2150 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2050 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 250 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1300 s

Figure 4, V. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria, grey, DIC. 420 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 336 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1758 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1932 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 630 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2460 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1046 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1624 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 532 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 210 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1110 s

Figure 4, V. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 210 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 850 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 200 s<br />

Figure 3, X. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 225 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2070 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 240 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 672 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1520 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 480s<br />

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 150 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1836 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 50 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 180 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 740 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 861 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1602 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1880 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1550 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 868 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1400 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 140 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 800 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 102 s

Figure 3, II. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 414 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 580 s

Figure 3, V. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 52 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1232 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1410 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1800 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1750 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1044 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 620 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 60 s<br />

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 783 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 860 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 450 s

Figure 3, VI. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2000 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 950 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 420 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1990 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 406 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 638 s

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1350 s

Figure 4, IX. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1036 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 300 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2352 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2040 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 400 s

Figure 4, III. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 350 s

Figure 4, IV. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Grey, DIC. 0 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 864 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 984 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 588 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 120 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 180 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 28 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1860 s

Figure 4, VII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. DIC, grey. 0 s<br />

Figure 4, VI. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1700 s

Figure 3, IV. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 2010 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 0 s

Figure 3, VIII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 900 s

Figure 4, II. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 350 s

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 108 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 432 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 520 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1770 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 224 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1100 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1407 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 950 s

Figure 3, IX. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1512 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1056 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 540 s<br />

Figure 3, III. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 939 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 784 s

Figure 3, VII. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1200 s

Figure 3, I. Vacuole and mitochondrial inheritance begins soon after bud emergence, at similar but not identical times. myo2∆ cells containing wild-type MYO2 on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 700 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 500 s

Figure 4, VIII. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 980 s

Figure 4, I. A Myo2 mutation that disrupts Mmr1 but not Vac17 binding exhibits a pronounced delay in mitochondria inheritance. myo2∆ cells containing myo2-I1308A on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1660 s

Figure 4, XI. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density &lt; 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 80 s

bioimagesImage 135524 (Figure 4 - Cell C (III): myo2-I1308A)Image 135509 (Figure 4 - Cell C (III): myo2-I1308A)Image 135275 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135813 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135835 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135784 (Figure 4 - Cell J (X): myo2-D1297N)Image 135824 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135802 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135750 (Figure 4 - Cell J (X): myo2-D1297N)Image 135769 (Figure 4 - Cell J (X): myo2-D1297N)Image 135296 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135322 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135338 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135172 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135421 (Figure 4 - Cell A (I): myo2-I1308A)Image 135535 (Figure 4 - Cell C (III): myo2-I1308A)Image 135291 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135442 (Figure 4 - Cell A (I): myo2-I1308A)Image 135354 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135572 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135311 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135550 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135681 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135450 (Figure 4 - Cell B (II): myo2-I1308A)Image 135644 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135620 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135472 (Figure 4 - Cell B (II): myo2-I1308A)Image 135161 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135748 (Figure 4 - Cell J (X): myo2-D1297N)Image 135494 (Figure 4 - Cell B (II): myo2-I1308A)Image 135693 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135700 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135655 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135722 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135548 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135392 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135383 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135300 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135434 (Figure 4 - Cell A (I): myo2-I1308A)Image 135502 (Figure 4 - Cell C (III): myo2-I1308A)Image 135401 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135419 (Figure 4 - Cell A (I): myo2-I1308A)Image 135346 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135348 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135707 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135646 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135468 (Figure 4 - Cell B (II): myo2-I1308A)Image 135580 (Figure 4 - Cell E (V): myo2-I1308A)Image 135638 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135166 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135479 (Figure 4 - Cell B (II): myo2-I1308A)Image 135618 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135629 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135605 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135776 (Figure 4 - Cell J (X): myo2-D1297N)Image 135670 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135366 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135791 (Figure 4 - Cell J (X): myo2-D1297N)Image 135248 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135598 (Figure 4 - Cell F (VI): myo2-I1308A)Figure 4 - Cell J (X): myo2-D1297NImage 135459 (Figure 4 - Cell B (II): myo2-I1308A)Image 135579 (Figure 4 - Cell E (V): myo2-I1308A)Image 135410 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135447 (Figure 4 - Cell A (I): myo2-I1308A)Image 135777 (Figure 4 - Cell J (X): myo2-D1297N)Image 135240 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135245 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135238 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135563 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135611 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135461 (Figure 4 - Cell B (II): myo2-I1308A)Image 135499 (Figure 4 - Cell C (III): myo2-I1308A)Image 135631 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135763 (Figure 4 - Cell J (X): myo2-D1297N)Image 135733 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135557 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135593 (Figure 4 - Cell E (V): myo2-I1308A)Image 135481 (Figure 4 - Cell B (II): myo2-I1308A)Image 135289 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135376 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135448 (Figure 4 - Cell A (I): myo2-I1308A)Image 135183 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135177 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135500 (Figure 4 - Cell C (III): myo2-I1308A)Image 135507 (Figure 4 - Cell C (III): myo2-I1308A)Image 135833 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135522 (Figure 4 - Cell C (III): myo2-I1308A)Image 135826 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135749 (Figure 4 - Cell J (X): myo2-D1297N)Image 135157 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135669 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135435 (Figure 4 - Cell A (I): myo2-I1308A)Image 135764 (Figure 4 - Cell J (X): myo2-D1297N)Figure 4 - Cell I (IX): myo2-D1297NImage 135815 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135804 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135277 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135526 (Figure 4 - Cell C (III): myo2-I1308A)Image 135273 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135463 (Figure 4 - Cell B (II): myo2-I1308A)Image 135452 (Figure 4 - Cell B (II): myo2-I1308A)Image 135426 (Figure 4 - Cell A (I): myo2-I1308A)Image 135664 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135755 (Figure 4 - Cell J (X): myo2-D1297N)Image 135181 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135373 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135412 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135422 (Figure 4 - Cell A (I): myo2-I1308A)Image 135305 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135581 (Figure 4 - Cell E (V): myo2-I1308A)Image 135658 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135152 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135146 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135675 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135474 (Figure 4 - Cell B (II): myo2-I1308A)Image 135552 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135385 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135622 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135695 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135768 (Figure 4 - Cell J (X): myo2-D1297N)Image 135492 (Figure 4 - Cell B (II): myo2-I1308A)Image 135568 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135716 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135381 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135642 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135724 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135368 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135414 (Figure 4 - Cell A (I): myo2-I1308A)Image 135266 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135170 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135686 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135439 (Figure 4 - Cell A (I): myo2-I1308A)Image 135286 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135316 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135585 (Figure 4 - Cell E (V): myo2-I1308A)Image 135603 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135756 (Figure 4 - Cell J (X): myo2-D1297N)Image 135282 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135406 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135511 (Figure 4 - Cell C (III): myo2-I1308A)Image 135427 (Figure 4 - Cell A (I): myo2-I1308A)Image 135757 (Figure 4 - Cell J (X): myo2-D1297N)Image 135515 (Figure 4 - Cell C (III): myo2-I1308A)Image 135513 (Figure 4 - Cell C (III): myo2-I1308A)Image 135518 (Figure 4 - Cell C (III): myo2-I1308A)Image 135229 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135561 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135586 (Figure 4 - Cell E (V): myo2-I1308A)Image 135231 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135633 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135483 (Figure 4 - Cell B (II): myo2-I1308A)Image 135327 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135333 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135705 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135428 (Figure 4 - Cell A (I): myo2-I1308A)Image 135735 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135559 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135587 (Figure 4 - Cell E (V): myo2-I1308A)Image 135743 (Figure 4 - Cell J (X): myo2-D1297N)Image 135842 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135180 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135226 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135528 (Figure 4 - Cell C (III): myo2-I1308A)Image 135415 (Figure 4 - Cell A (I): myo2-I1308A)Image 135657 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135314 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135287 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135649 (Figure 4 - Cell G (VII): myo2-D1297N)Figure 3 - Cell C (III): Wild-type Myo2Image 135430 (Figure 4 - Cell A (I): myo2-I1308A)Image 135790 (Figure 4 - Cell J (X): myo2-D1297N)Image 135153 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135740 (Figure 4 - Cell I (IX): myo2-D1297N)Figure 3 - Cell A (I): Wild-type Myo2Image 135465 (Figure 4 - Cell B (II): myo2-I1308A)Image 135652 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135454 (Figure 4 - Cell B (II): myo2-I1308A)Image 135487 (Figure 4 - Cell B (II): myo2-I1308A)Image 135476 (Figure 4 - Cell B (II): myo2-I1308A)Image 135602 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135689 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135446 (Figure 4 - Cell A (I): myo2-I1308A)Image 135531 (Figure 4 - Cell C (III): myo2-I1308A)Image 135747 (Figure 4 - Cell J (X): myo2-D1297N)Image 135280 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135532 (Figure 4 - Cell C (III): myo2-I1308A)Image 135772 (Figure 4 - Cell J (X): myo2-D1297N)Image 135271 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135539 (Figure 4 - Cell C (III): myo2-I1308A)Image 135576 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135386 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135566 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135668 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135554 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135387 (Figure 3 - Cell I (IX): Wild-type Myo2)Figure 3 - Cell E (V): Wild-type Myo2Image 135486 (Figure 4 - Cell B (II): myo2-I1308A)Image 135678 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135641 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135325 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135364 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135380 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135544 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135640 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135335 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135582 (Figure 4 - Cell E (V): myo2-I1308A)Image 135704 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135714 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135810 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135788 (Figure 4 - Cell J (X): myo2-D1297N)Image 135820 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135169 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135520 (Figure 4 - Cell C (III): myo2-I1308A)Image 135832 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135370 (Figure 3 - Cell H (VIII): Wild-type Myo2)Figure 4 - Cell E (V): myo2-I1308AImage 135527 (Figure 4 - Cell C (III): myo2-I1308A)Image 135529 (Figure 4 - Cell C (III): myo2-I1308A)Image 135294 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135420 (Figure 4 - Cell A (I): myo2-I1308A)Image 135648 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135667 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135143 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135243 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135690 (Figure 4 - Cell H (VIII): myo2-D1297N)Figure 3 - Cell G (VII): Wild-type Myo2Image 135464 (Figure 4 - Cell B (II): myo2-I1308A)Image 135475 (Figure 4 - Cell B (II): myo2-I1308A)Image 135679 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135488 (Figure 4 - Cell B (II): myo2-I1308A)Image 135651 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135455 (Figure 4 - Cell B (II): myo2-I1308A)Image 135402 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135530 (Figure 4 - Cell C (III): myo2-I1308A)Image 135436 (Figure 4 - Cell A (I): myo2-I1308A)Image 135656 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135303 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135592 (Figure 4 - Cell E (V): myo2-I1308A)Image 135350 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135154 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135577 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135601 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135553 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135485 (Figure 4 - Cell B (II): myo2-I1308A)Image 135762 (Figure 4 - Cell J (X): myo2-D1297N)Image 135543 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135269 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135567 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135279 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135653 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135703 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135778 (Figure 4 - Cell J (X): myo2-D1297N)Image 135413 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135713 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135843 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135840 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135822 (Figure 4 - Cell K (XI): myo2-D1297N)Figure 3 - Cell J (X): Wild-type Myo2Image 135811 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135236 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135800 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135302 (Figure 3 - Cell E (V): Wild-type Myo2)Figure 4 - Cell A (I): myo2-I1308AImage 135688 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135292 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135371 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135770 (Figure 4 - Cell J (X): myo2-D1297N)Image 135467 (Figure 4 - Cell B (II): myo2-I1308A)Image 135590 (Figure 4 - Cell E (V): myo2-I1308A)Image 135456 (Figure 4 - Cell B (II): myo2-I1308A)Image 135168 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135786 (Figure 4 - Cell J (X): myo2-D1297N)Image 135650 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135478 (Figure 4 - Cell B (II): myo2-I1308A)Image 135404 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135534 (Figure 4 - Cell C (III): myo2-I1308A)Image 135533 (Figure 4 - Cell C (III): myo2-I1308A)Image 135388 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135261 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135397 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135281 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135538 (Figure 4 - Cell C (III): myo2-I1308A)Image 135792 (Figure 4 - Cell J (X): myo2-D1297N)Image 135574 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135600 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135270 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135496 (Figure 4 - Cell B (II): myo2-I1308A)Image 135537 (Figure 4 - Cell C (III): myo2-I1308A)Image 135564 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135630 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135390 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135546 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135730 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135742 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135556 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135720 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135702 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135444 (Figure 4 - Cell A (I): myo2-I1308A)Image 135179 (Figure 3 - Cell A (I): Wild-type Myo2)Figure 3 - Cell F (VI): Wild-type Myo2Image 135830 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135712 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135542 (Figure 4 - Cell C (III): myo2-I1308A)Image 135540 (Figure 4 - Cell C (III): myo2-I1308A)Image 135841 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135794 (Figure 4 - Cell J (X): myo2-D1297N)Image 135801 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135440 (Figure 4 - Cell A (I): myo2-I1308A)Image 135780 (Figure 4 - Cell J (X): myo2-D1297N)Image 135155 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135237 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135268 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135466 (Figure 4 - Cell B (II): myo2-I1308A)Image 135477 (Figure 4 - Cell B (II): myo2-I1308A)Image 135324 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135457 (Figure 4 - Cell B (II): myo2-I1308A)Image 135666 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135336 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135654 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135178 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135782 (Figure 4 - Cell J (X): myo2-D1297N)Image 135365 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135313 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135293 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135565 (Figure 4 - Cell D (IV): myo2-I1308A)Figure 4 - Cell F (VI): myo2-I1308AImage 135144 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135497 (Figure 4 - Cell B (II): myo2-I1308A)Image 135575 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135677 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135288 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135167 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135278 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135741 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135545 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135731 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135227 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135555 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135721 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135403 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135711 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135821 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135541 (Figure 4 - Cell C (III): myo2-I1308A)Figure 3 - Cell D (IV): Wild-type Myo2Image 135701 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135831 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135306 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135589 (Figure 4 - Cell E (V): myo2-I1308A)Image 135411 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135424 (Figure 4 - Cell A (I): myo2-I1308A)Image 135267 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135708 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135719 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135145 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135676 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135347 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135283 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135469 (Figure 4 - Cell B (II): myo2-I1308A)Image 135458 (Figure 4 - Cell B (II): myo2-I1308A)Image 135628 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135617 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135606 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135660 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135372 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135599 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135766 (Figure 4 - Cell J (X): myo2-D1297N)Image 135358 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135781 (Figure 4 - Cell J (X): myo2-D1297N)Image 135437 (Figure 4 - Cell A (I): myo2-I1308A)Image 135787 (Figure 4 - Cell J (X): myo2-D1297N)Figure 4 - Cell C (III): myo2-I1308AImage 135301 (Figure 3 - Cell E (V): Wild-type Myo2)Figure 4 - Cell B (II): myo2-I1308AImage 135156 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135562 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135610 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135536 (Figure 4 - Cell C (III): myo2-I1308A)Image 135260 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135377 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135460 (Figure 4 - Cell B (II): myo2-I1308A)Image 135632 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135665 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135732 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135645 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135482 (Figure 4 - Cell B (II): myo2-I1308A)Image 135671 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135583 (Figure 4 - Cell E (V): myo2-I1308A)Image 135391 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135405 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135710 (Figure 4 - Cell I (IX): myo2-D1297N)Figure 4 - Cell K (XI): myo2-D1297NImage 135558 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135753 (Figure 4 - Cell J (X): myo2-D1297N)Image 135525 (Figure 4 - Cell C (III): myo2-I1308A)Image 135659 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135774 (Figure 4 - Cell J (X): myo2-D1297N)Image 135823 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135508 (Figure 4 - Cell C (III): myo2-I1308A)Image 135523 (Figure 4 - Cell C (III): myo2-I1308A)Image 135836 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135812 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135367 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135445 (Figure 4 - Cell A (I): myo2-I1308A)Image 135760 (Figure 4 - Cell J (X): myo2-D1297N)Image 135779 (Figure 4 - Cell J (X): myo2-D1297N)Image 135803 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135312 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135682 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135151 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135451 (Figure 4 - Cell B (II): myo2-I1308A)Image 135699 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135431 (Figure 4 - Cell A (I): myo2-I1308A)Image 135328 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135249 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135230 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135591 (Figure 4 - Cell E (V): myo2-I1308A)Image 135796 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135432 (Figure 4 - Cell A (I): myo2-I1308A)Image 135244 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135573 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135687 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135317 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135228 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135297 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135323 (Figure 3 - Cell E (V): Wild-type Myo2)Figure 4Image 135471 (Figure 4 - Cell B (II): myo2-I1308A)Image 135498 (Figure 4 - Cell C (III): myo2-I1308A)Image 135337 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135692 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135621 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135717 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135232 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135501 (Figure 4 - Cell C (III): myo2-I1308A)Image 135547 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135723 (Figure 4 - Cell I (IX): myo2-D1297N)Figure 3Image 135584 (Figure 4 - Cell E (V): myo2-I1308A)Image 135680 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135503 (Figure 4 - Cell C (III): myo2-I1308A)Image 135706 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135160 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135429 (Figure 4 - Cell A (I): myo2-I1308A)Image 135647 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135321 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135746 (Figure 4 - Cell J (X): myo2-D1297N)Image 135326 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135334 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135345 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135489 (Figure 4 - Cell B (II): myo2-I1308A)Image 135560 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135571 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135604 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135176 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135510 (Figure 4 - Cell C (III): myo2-I1308A)Image 135619 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135417 (Figure 4 - Cell A (I): myo2-I1308A)Image 135767 (Figure 4 - Cell J (X): myo2-D1297N)Image 135171 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135295 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135578 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135685 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135332 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135612 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135691 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135752 (Figure 4 - Cell J (X): myo2-D1297N)Image 135315 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135396 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135165 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135339 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135462 (Figure 4 - Cell B (II): myo2-I1308A)Image 135634 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135643 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135484 (Figure 4 - Cell B (II): myo2-I1308A)Image 135423 (Figure 4 - Cell A (I): myo2-I1308A)Image 135349 (Figure 3 - Cell F (VI): Wild-type Myo2)Figure 3 - Cell B (II): Wild-type Myo2Image 135438 (Figure 4 - Cell A (I): myo2-I1308A)Image 135521 (Figure 4 - Cell C (III): myo2-I1308A)Image 135734 (Figure 4 - Cell I (IX): myo2-D1297N)Figure 3 - Cell H (VIII): Wild-type Myo2Image 135182 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135147 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135276 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135304 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135834 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135235 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135382 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135290 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135375 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135825 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135425 (Figure 4 - Cell A (I): myo2-I1308A)Image 135759 (Figure 4 - Cell J (X): myo2-D1297N)Image 135814 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135674 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135754 (Figure 4 - Cell J (X): myo2-D1297N)Image 135805 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135588 (Figure 4 - Cell E (V): myo2-I1308A)Image 135374 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135453 (Figure 4 - Cell B (II): myo2-I1308A)Figure 3 - Cell I (IX): Wild-type Myo2Image 135416 (Figure 4 - Cell A (I): myo2-I1308A)Image 135262 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135389 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135745 (Figure 4 - Cell J (X): myo2-D1297N)Image 135272 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135516 (Figure 4 - Cell C (III): myo2-I1308A)Image 135514 (Figure 4 - Cell C (III): myo2-I1308A)Image 135517 (Figure 4 - Cell C (III): myo2-I1308A)Image 135351 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135512 (Figure 4 - Cell C (III): myo2-I1308A)Image 135519 (Figure 4 - Cell C (III): myo2-I1308A)Image 135400 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135158 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135239 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135241 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135551 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135623 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135473 (Figure 4 - Cell B (II): myo2-I1308A)Image 135758 (Figure 4 - Cell J (X): myo2-D1297N)Image 135310 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135639 (Figure 4 - Cell G (VII): myo2-D1297N)Image 135663 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135694 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135493 (Figure 4 - Cell B (II): myo2-I1308A)Image 135569 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135715 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135549 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135725 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135341 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135775 (Figure 4 - Cell J (X): myo2-D1297N)Image 135806 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135449 (Figure 4 - Cell A (I): myo2-I1308A)Image 135817 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135684 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135839 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135505 (Figure 4 - Cell C (III): myo2-I1308A)Image 135744 (Figure 4 - Cell J (X): myo2-D1297N)Image 135828 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135635 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135624 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135613 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135263 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135379 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135164 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135343 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135595 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135408 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135799 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135319 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135356 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135352 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135299 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135309 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135395 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135369 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135443 (Figure 4 - Cell A (I): myo2-I1308A)Image 135394 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135793 (Figure 4 - Cell J (X): myo2-D1297N)Image 135159 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135175 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135398 (Figure 3 - Cell J (X): Wild-type Myo2)Figure 4 - Cell G (VII): myo2-D1297NImage 135726 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135418 (Figure 4 - Cell A (I): myo2-I1308A)Image 135185 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135490 (Figure 4 - Cell B (II): myo2-I1308A)Image 135736 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135506 (Figure 4 - Cell C (III): myo2-I1308A)Image 135504 (Figure 4 - Cell C (III): myo2-I1308A)Image 135331 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135807 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135816 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135827 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135308 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135662 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135344 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135614 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135625 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135247 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135246 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135570 (Figure 4 - Cell D (IV): myo2-I1308A)Image 135765 (Figure 4 - Cell J (X): myo2-D1297N)Image 135399 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135174 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135594 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135609 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135751 (Figure 4 - Cell J (X): myo2-D1297N)Image 135342 (Figure 3 - Cell F (VI): Wild-type Myo2)Image 135148 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135340 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135320 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135696 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135491 (Figure 4 - Cell B (II): myo2-I1308A)Image 135673 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135727 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135163 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135433 (Figure 4 - Cell A (I): myo2-I1308A)Image 135407 (Figure 3 - Cell J (X): Wild-type Myo2)Image 135737 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135234 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135149 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135837 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135265 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135819 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135285 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135808 (Figure 4 - Cell K (XI): myo2-D1297N)Figure 4 - Cell D (IV): myo2-I1308AImage 135795 (Figure 4 - Cell J (X): myo2-D1297N)Image 135318 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135698 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135789 (Figure 4 - Cell J (X): myo2-D1297N)Image 135637 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135626 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135672 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135615 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135441 (Figure 4 - Cell A (I): myo2-I1308A)Image 135357 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135597 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135184 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135359 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135608 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135761 (Figure 4 - Cell J (X): myo2-D1297N)Image 135797 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135353 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135355 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135661 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135480 (Figure 4 - Cell B (II): myo2-I1308A)Image 135773 (Figure 4 - Cell J (X): myo2-D1297N)Image 135738 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135728 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135329 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135470 (Figure 4 - Cell B (II): myo2-I1308A)Image 135384 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135393 (Figure 3 - Cell I (IX): Wild-type Myo2)Image 135409 (Figure 3 - Cell J (X): Wild-type Myo2)Figure 4 - Cell H (VIII): myo2-D1297NImage 135838 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135258 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135809 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135259 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135785 (Figure 4 - Cell J (X): myo2-D1297N)Image 135298 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135718 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135250 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135818 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135829 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135697 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135709 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135636 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135162 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135251 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135252 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135616 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135627 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135256 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135257 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135254 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135253 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135274 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135284 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135363 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135607 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135264 (Figure 3 - Cell D (IV): Wild-type Myo2)Image 135771 (Figure 4 - Cell J (X): myo2-D1297N)Image 135255 (Figure 3 - Cell C (III): Wild-type Myo2)Image 135596 (Figure 4 - Cell F (VI): myo2-I1308A)Image 135307 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135360 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135361 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135378 (Figure 3 - Cell H (VIII): Wild-type Myo2)Image 135362 (Figure 3 - Cell G (VII): Wild-type Myo2)Image 135798 (Figure 4 - Cell K (XI): myo2-D1297N)Image 135330 (Figure 3 - Cell E (V): Wild-type Myo2)Image 135150 (Figure 3 - Cell A (I): Wild-type Myo2)Image 135683 (Figure 4 - Cell H (VIII): myo2-D1297N)Image 135233 (Figure 3 - Cell B (II): Wild-type Myo2)Image 135729 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135739 (Figure 4 - Cell I (IX): myo2-D1297N)Image 135783 (Figure 4 - Cell J (X): myo2-D1297N)Image 135173 (Figure 3 - Cell A (I): Wild-type Myo2)Yui JinLois S. WeismanMatthew BrunnerP. Taylor EvesfalseOverlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes2012-07-02T11:22:57Z2018-11-29T11:22:57Z2018-11-29T11:22:57ZS-JCBD-20120102410.1083/jcb.201201024