{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sandra Dieni"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201201038"],"attach_to":["JCB"],"legend":["Electron micrograph of an ultrathin section of a wild-type mouse stratum lucidum pre-labeled with anti-BDNF immunogold particles.","Pro-BDNF antibodies (green) do not produce an immunoreactive signal in the CA3 region of a cbdnf-/- mouse hippocampus.","Anti-pro-BDNF-Mab5H8-IR (green) labeling is markedly increased in the hilus of the dentate gyrus in a Bsn mouse mutant.","Electron micrograph of a cbdnf-/- mouse dendritic  profile in the stratum lucidum labeled with anti-pro-BDNF-immunogold antibodies.","Pro-BDNF antibodies (green) do not produce an immunoreactive signal in the dentate gyrus region of a cbdnf-/- mouse hippocampus.","An electron micrograph of anti-BDNF-immunogold labeling of sections from cbdnf-/- mice yielded non-specific background labeling.","Anti-BDNF-IR (red) labeling is markedly increased in the CA3 area of a Bsn mouse mutant.","Low power confocal z-stack of the dentate gyrus of a wild-type mouse hippocampus. The same subset of granule cells co-expresses  BDNF-IR (red) and pro-BDNF-IR (green).","Electron micrograph showing the Golgi complex of a wild-type mouse CA3 neuron labeled with anti-pro-BDNF immunogold. Clusters of gold grains aggregate around the cisterns of the Golgi apparatus.","Anti-BDNF-IR (red) labeling is markedly increased in the hilus of the dentate gyrus of a Bsn mouse mutant.","High resolution confocal z-slice of the stratum radiatum in the CA1 region of a BDNF-Myc mouse. Overlay of anti-Myc (red) and anti-synaptopodin (green) labeling shows presynaptic localization of BDNF, with close apposition of Myc-IR with synpo-positive spines.","Confocal z-slice of a wild-type mouse stratum lucidum labeled with antibodies to Myc (red) and VGLUT-1 (green).","Myc-IR (red) and  CCK-IR (green) in presynaptic terminals within a wild-type mouse stratum lucidum. The merged image indicates complete segregation of the two peptides in the mossy fiber projection.","A small proportion of mossy fiber boutons in the wild-type mouse stratum lucidum co-expresses Myc-IR (red) and Met-enk-IR (green). However, in the merged image (shown), Myc- and Met-enk-IR puncta do not overlap.","Electron micrograph of mossy fiber boutons from a Bsn mouse mutant section pre-labeled with BDNF immunogold.","In sections processed for anti-BDNF immunogold, gold-labeled vesicles were observed in electron micrographs of wild-type mouse unmyelinated mossy fiber axons.","Electron micrograph of a mossy fiber bouton from a wild-type mouse section pre-labeled with anti-pro-BDNF immunogold.","Electron micrograph of a wild-type mouse dendrite labeled with pro-BDNF-immunogold antibodies.","Electron micrograph of a wild-type mouse dendritic profile in the stratum lucidum labeled with anti-pro-BDNF-immunogold antibodies.","Immunoprecipitation with anti-BDNF antisera and Western blot analysis with anti-BDNF N-20 antibody demonstrating the recovery of wild-type recombinant pro-BDNF (upper bands, lanes 2,5) and mature BDNF (lower bands, lanes 2,5) from mouse hippocampal lysates in the absence (lane 2) or presence (lane 5) of protease inhibitors. Lanes 1, 3 and 4 represent control samples (1 = +wild-type HIP only; 3 = +protease inhibitors, +recombinant BDNF; 4 = +wild-type HIP, +protease inhibitors).","Low power images of a wild-type mouse dentate gyrus labeled with anti-BDNF (red) and anti-pro-BDNF-(green) antibodies.","High magnification view of the dentate gyrus region of a cbdnf-/- mouse hippocampus stained with anti-BDNF antibodies (red). Note the absence of immunoreactive signals in all cellular and neuropil layers.","No BDNF-positive secretory vesicles were found by immunogold labeling in a section from a cbdnf-/- mice visualized in this electron micrograph.","In the stratum lucidum of a Bsn mouse mutant, an increased density of labeled profiles was observed, with  BDNF-IR (green) labeling showing virtually no overlap with labeling for the post-synaptic marker MAP-2 (red).","Low power view of a wild-type mouse hippocampal section stained with anti-BDNF antibodies (red).","A high resolution confocal z-slice shows co-localization of BDNF-IR (red) and pro-BDNF-IR (green) labeling at the border of stratum radiatum and stratum lacunosum-moleculare in a section from a Bsn mutant mouse.","No pro-BDNF-positive secretory vesicles were found by immunogold labeling in this electron micrograph of a section from a cbdnf-/- mice.","Anti-BDNF-positive granule cells in a Bsn mouse mutant shows labeling confined to the cell soma and no detectable dendritic staining in the inner molecular layer.","Electron micrograph of a Bsn mouse mutant dendrite labeled with anti-BDNF immunogold.","Low power image of a wild-type mouse CA3 region labeled with anti-pro-BDNF-Mab5H8 (green) antibodies.","No specific staining is observed in the dentate gyrus region of a wild-type mouse hippocampus section treated with anti-Myc antibodies (red).","Electron micrograph of a cbdnf-/- mouse dendritic  profile in the stratum lucidum labeled with anti-BDNF-immunogold antibodies.","Merged confocal z-slice of a wild-type mouse stratum lucidum labeled with antibodies to  Myc (red) and Synaptophysin (green).","Electron micrograph of a wild-type mouse dendrite labeled with anti-BDNF immunogold.","Anti-pro-BDNF-Mab5H8-positive granule cells (green) in a Bsn mouse mutant shows labeling confined to the cell soma and no detectable dendritic staining in the inner molecular layer.","Electron micrograph of a Bsn mouse mutant dendrite labeled with pro-BDNF immunogold.","Shown is Myc-IR (red) and MAP-2-IR (green) labeling in a confocal z-stack from a wild-type mouse CA3 region highlighting Myc-positive neuronal somata  in the PCL and mossy fiber bouton profiles in the stratum lucidum. No overlap between Myc and MAP2 labeling can be seen.","Electron micrograph of a mossy fiber bouton from a Bsn mouse mutant section pre-labeled with anti- pro-BDNF immunogold.","Low power image of a wild-type mouse CA3 region labeled with anti-BDNF antibodies (red).","Anti-pro-BDNF-Mab5H8-IR (green) labeling is markedly increased in the CA3 area of a Bsn mouse mutant.","A confocal stack of a BDNF-(red) and pro-BDNF (green)-immunoreactive granule cell counterstained with DAPI (blue) from a wild-type mouse hippocampus. Labeling concentrated at the cell apex in distinct clusters, with partial overlap of the BDNF and pro-BDNF signals.","Electron micrograph of a mossy fiber bouton from a wild-type mouse section  pre-labeled with anti-BDNF immunogold.","BDNF-IR (red) and pro-BDNF-IR (green) labeling in a Bsn mouse mutant shows a laminar-specific increase in staining at the border of stratum radiatum and stratum laconosum-moleculare.","Electron micrograph of a wild-type mouse dendritic  profile in the stratum lucidum labeled with anti-BDNF-immunogold antibodies.","In stratum lucidum of a Bsn mouse mutant, an increased density of labeled profiles was observed, with BDNF-IR (red) labeling showing virtually no overlap with labeling for the post-synaptic marker Synaptopodin (green).","High resolution confocal z-stack of a wild-type mouse dentate gyrus labeled with antibodies against BDNF (red), pro-BDNF (green) and Arc/Arg3.1 (blue).","No specific staining in the CA3 region of a wild-type mouse hippocampus section treated with anti-Myc antibodies (red).","Both pro-BDNF and its pro-peptide were detected by Western blotting of mouse hippocampal lysates immunoprecipitated with anti-pro-BDNF antibody and detected with anti-pro-BDNF monoclonal antibody 5H8 in wildtype (lane 1) but not in cbdnf-/- mouse samples (lane 2). Cleavage-resistant pro-BDNF (lane 3), recombinant BDNF pro-peptide (lanes 4 & 5) and recombinant non-glycosylated pro-peptide (lane 6) were used as molecular weight markers.","A Bdnf-Myc-expressing mouse hippocampus section treated with anti-Myc antibodies (red) shows a similar staining pattern to that produced by anti-BDNF antibodies.","Polyclonal pro-BDNF antibodies (green) yield a similar pattern to that of anti-BDNF in a wild-type mouse hippocampus section.","Co-labeling of the Bsn mouse mutant CA1 region with antibodies against BDNF (red) and Synaptopodin (green) reveals a segregation of  signals at the border of stratum radiatum and stratum lacunosum-moleculare.","Co-labeling of a Bsn mouse mutant CA1 region with antibodies against BDNF (red) and GFAP (green) shows a lack of BDNF-IR in astrocytes in a confocal z-stack.","High resolution confocal z-stack of a single CA3 neuron from a wild-type mouse hippocampus labeled with antibodies against Myc (red),  pro-BDNF (green) and GM130 (light blue).","Electron micrograph of an anti-pro-BDNF immunogold-labeled wild-type mouse mossy fiber bouton containing cluster-labeled secretory vesicles.","Electron micrograph showing anti-BDNF-immunogold labeling of a mouse presynaptic terminal in the stratum radiatum of CA1.","Immunoprecipitation with anti-BDNF antisera and Western blot analysis with anti-BDNF N20 antibody of hippocampal lysates from Bsn mouse mutants revealed a relatively similar increase (~3 fold) in the levels of BDNF and pro-BDNF (lane 3) compared to wild-type lysates (lanes 1 and 2). Recombinant mouse BDNF (lanes 4-6, 8, 10) was used as a molecular weight marker.","Confocal z-slice of area CA3 of a wild-type mouse hippocampus, demonstrating BDNF-IR (red) and  pro-BDNF-IR (green) in a subset of CA3 pyramidal cell somata. DAPI staining is shown in blue.","Confocal z-stack of a wild-type mouse granule cell co-expressing  Myc-IR (red) and Met-enk-IR (green). Puncta are similarly distributed throughout the cell but show little overlap.","Immunoprecipitation with anti-pro-BDNF antisera and Western blot analysis with anti-pro-BDNF monoclonal antibody 5H8 of hippocampal lysates from Bsn mouse mutants revealed a relatively similar increase (~3 fold) in the levels of  pro-peptide and pro-BDNF (lane 2), compared to wild-type tissues (lane 1). Recombinant BDNF pro-peptide (lanes 3,4,5)  and cleavage-resistant pro-BDNF (lanes 3,4,6) were used as molecular weight markers.","High resolution confocal z-slice of a wild-type mouse stratum lucidum showing Myc-IR (red) and  pro-BDNF-IR (green) in the same subset of mossy fiber boutons.","Higher magnification view of the CA3 region of a cbdnf-/- mouse hippocampus stained with anti-BDNF antibodies (red). Note the absence of immunoreactive signals in all cellular and neuropil layers.","Electron micrograph showing immunogold labeling of an anti-pro-BDNF-positive vesicle within a wild-type mouse mossy fiber that can been seen terminating into a bouton."],"repository":["bioimages"],"figure_sub":["Image 38335 (Figure 5 - D)","Figure 8 - C","Image 38337 (Figure 6 - H)","Figure 8 - D","Figure 8 - A","Figure 8 - B","Image 38301 (Figure 1 - C)","Image 38339 (Figure 6 - K)","Image 38427 (Supplemental Figure 1 - D)","Image 38388 (Figure 2 - A-C)","Image 38420 (Figure 7 - G, H)","Figure 10 - C, left panel","Image 38349 (Figure 9 - B)","Image 38308 (Figure 1 - J)","Image 38419 (Figure 7 - G, H)","Image 38329 (Figure 4 - D)","Figure 2 - G-J","Figure 1 - I","Figure 1 - H","Image 38418 (Figure 7 - E, F)","Figure 1 - G","Figure 1 - F","Figure 3 - Q-S","Image 38426 (Supplemental Figure 1 - A-C)","Image 38306 (Figure 1 - H)","Figure 1 - J","Image 38392 (Figure 2 - G-J)","Figure 1 - E","Figure 1 - D","Figure 1 - C","Image 38342 (Figure 8 - B)","Figure 1 - B","Figure 7 - I, J","Figure 10 - C, right panel","Figure 7 - A, B","Image 38330 (Figure 4 - A)","Image 38401 (Figure 3 - G)","Image 38327 (Figure 4 - B)","Image 38320 (Figure 2 - K)","Figure 3 - D","Figure 3 - E","Figure 3 - F","Figure 3 - G","Image 133118 (Supplemental Figure 2 - None)","Figure 6 - A-C","Image 38305 (Figure 1 - G)","Figure 2 - D-F","Image 38340 (Figure 6 - L)","Image 38428 (Supplemental Figure 1 - E)","Image 38396 (Figure 3 - E)","Figure 5 - A","Figure 5 - B","Figure 5 - C","Figure 5 - D","Image 38422 (Figure 7 - I, J)","Figure 5","Image 38429 (Supplemental Figure 1 - F)","Figure 4","Figure 7 - G, H","Supplemental Figure 2 - None","Figure 7","Figure 6","Figure 10","Figure 9","Figure 8","Image 38303 (Figure 1 - E)","Figure 3 - H-J","Image 38351 (Figure 10 - A)","Image 38333 (Figure 5 - B)","Figure 1","Figure 2 - A-C, inset","Figure 3","Supplemental Figure 1 - A-C","Figure 2","Supplemental Figure 1 - F","Figure 10 - A","Supplemental Figure 2","Image 38307 (Figure 1 - I)","Supplemental Figure 1 - E","Supplemental Figure 1","Supplemental Figure 1 - D","Image 38409 (Figure 3 - Q-S)","Image 38328 (Figure 4 - C)","Image 38416 (Figure 7 - C, D)","Figure 9 - B","Figure 9 - C","Image 38437 (Figure 10 - C, left panel)","Figure 9 - D","Image 38300 (Figure 1 - B)","Figure 9 - A","Image 38338 (Figure 6 - I)","Image 38341 (Figure 8 - A)","Image 38393 (Figure 3 - A-C)","Image 38405 (Figure 3 - K-M)","Figure 3 - K-M","Image 38424 (Figure 7 - K, L)","Figure 2 - K","Figure 7 - E, F","Image 38415 (Figure 7 - C, D)","Image 38389 (Figure 2 - A-C, inset)","Image 38408 (Figure 3 - N-P)","Image 38343 (Figure 8 - C)","Image 38414 (Figure 7 - A, B)","Figure 3 - A-C","Image 38347 (Figure 9 - A)","Image 38348 (Figure 9 - C)","Image 38304 (Figure 1 - F)","Image 38411 (Figure 6 - A-C)","Image 38332 (Figure 5 - A)","Image 38425 (Figure 7 - K, L)","Image 38395 (Figure 3 - D)","Image 38417 (Figure 7 - E, F)","Image 38438 (Figure 10 - C, right panel)","Image 38344 (Figure 8 - D)","Figure 4 - A","Figure 4 - B","Image 38444 (Figure 6 - D-G)","Image 38421 (Figure 7 - I, J)","Figure 4 - C","Figure 4 - D","Figure 6 - K","Figure 6 - L","Figure 2 - A-C","Figure 6 - I","Image 38399 (Figure 3 - F)","Figure 3 - N-P","Figure 7 - K, L","Image 38404 (Figure 3 - H-J)","Image 38334 (Figure 5 - C)","Figure 6 - D-G","Image 38350 (Figure 9 - D)","Image 38302 (Figure 1 - D)","Figure 7 - C, D","Figure 6 - H","Image 38391 (Figure 2 - D-F)"],"pubmed_authors":["Ruben Deogracias","Masami Kojima","Michael Frotscher","Tomoya Matsumoto","Yves-Alain Barde","Martijn Dekkers","Stefanie Rauskolb","Sandra Dieni","Eckart D. Gundelfinger","Sigrun Nestel","Mihai S. Ionescu"],"additional_accession":[]},"is_claimable":false,"name":"BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons","description":null,"dates":{"release":"2012-03-12T11:23:11Z","modification":"2018-11-29T11:23:11Z","creation":"2018-11-29T11:23:11Z"},"accession":"S-JCBD-201201038","cross_references":{"doi":["10.1083/jcb.201201038"]}}