{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ryan J. Petrie"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201201124"],"attach_to":["JCB"],"legend":["A Z-stack of a human foreskin fibroblast (HFF) expressing YFP-paxillin migrating inside 3D 1.7 mg/ml collagen.","Z-stack of a llobopodia-bearing human foreskin fibroblast (HFF) expressing GFP-actin migrating in collagen; GFP-actin in green and second harmonic imaging of collagen in grayscale.","Z-stack of a human foreskin fibroblast (HFF) expressing GFP using lamellipodia-based migration on 2D cell derived matrix (CDM; Alexa633, red).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating on top of 2D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating on top of 2D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating in 3D 1.7 mg/ml collagen. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET (donor excitation/acceptor emission; purple).","A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating in 3D cell-derived matrix (Alexa633, red).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating in 3D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET (donor excitation/acceptor emission; purple).","Z-stack of a human foreskin fibroblast (HFF) expressing GFP (green) using lamellipodia-based migration inside 3D 1.7 mg/ml collagen (reflection microscopy, red).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating in 3D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).","A Z-stack of a human foreskin fibroblast (HFF) expressing YFP-paxillin (green) migrating inside 3D CDM (Alexa633; red).","A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating on top of 2D cell-derived matrix (Alexa633, red).","A 2x2 tiled z-stack of a mouse ear dermal explant labeled with Alexa633.","Z-stack of a human foreskin fibroblast (HFF) expressing GFP (green) using 3D lobopodia-based migration inside cell-derived matrix (CDM; Alexa633, red).","A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating in 3D 1.7 mg/ml collagen. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).","A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating in 3D 1.7 mg/ml collagen (reflection microscopy, red)."],"repository":["bioimages"],"figure_sub":["Figure 3 - E","Image 134067 (Figure 3 - A)","Figure 3 - A","Image 134062 (Figure 1 - B)","Image 134075 (Figure 3 - E)","Figure 3 - C","Image 134071 (Figure 3 - C)","Image 134064 (Figure 2 - D)","Image 134068 (Figure 3 - A)","Image 134072 (Figure 3 - C)","Image 134069 (Figure 3 - A)","Image 134076 (Figure 5 - E)","Image 134065 (Figure 2 - D)","Figure 1 - C","Figure 1 - B","Image 134073 (Figure 3 - E)","Figure 5 - E","Figure 5","Image 134077 (Figure 5 - E)","Image 134066 (Figure 2 - D)","Figure 1","Figure 2 - D","Image 134063 (Figure 1 - C)","Figure 3","Image 134070 (Figure 3 - C)","Figure 2","Image 134074 (Figure 3 - E)"],"pubmed_authors":["Ryan J. Petrie","Núria Gavara","Richard S. Chadwick","Kenneth M. Yamada"],"additional_accession":[]},"is_claimable":false,"name":"Nonpolarized signaling reveals two distinct modes of 3D cell migration","description":null,"dates":{"release":"2012-04-30T11:23:13Z","modification":"2018-11-29T11:23:13Z","creation":"2018-11-29T11:23:13Z"},"accession":"S-JCBD-201201124","cross_references":{"doi":["10.1083/jcb.201201124"]}}