bioimagesUnknownRyan J. PetrieThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201201124JCB

A Z-stack of a human foreskin fibroblast (HFF) expressing YFP-paxillin migrating inside 3D 1.7 mg/ml collagen.

Z-stack of a llobopodia-bearing human foreskin fibroblast (HFF) expressing GFP-actin migrating in collagen; GFP-actin in green and second harmonic imaging of collagen in grayscale.

Z-stack of a human foreskin fibroblast (HFF) expressing GFP using lamellipodia-based migration on 2D cell derived matrix (CDM; Alexa633, red).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating on top of 2D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating on top of 2D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating in 3D 1.7 mg/ml collagen. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET (donor excitation/acceptor emission; purple).

A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating in 3D cell-derived matrix (Alexa633, red).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Rac1 biosensor (CFP-Rac1 and YPet-PBD) migrating in 3D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET (donor excitation/acceptor emission; purple).

Z-stack of a human foreskin fibroblast (HFF) expressing GFP (green) using lamellipodia-based migration inside 3D 1.7 mg/ml collagen (reflection microscopy, red).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating in 3D cell-derived matrix. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).

A Z-stack of a human foreskin fibroblast (HFF) expressing YFP-paxillin (green) migrating inside 3D CDM (Alexa633; red).

A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating on top of 2D cell-derived matrix (Alexa633, red).

A 2x2 tiled z-stack of a mouse ear dermal explant labeled with Alexa633.

Z-stack of a human foreskin fibroblast (HFF) expressing GFP (green) using 3D lobopodia-based migration inside cell-derived matrix (CDM; Alexa633, red).

A Z-stack of a human foreskin fibroblast (HFF) expressing the intramolecular FRET-based Cdc42 biosensor (CFP-Cdc42 and YPet-PBD) migrating in 3D 1.7 mg/ml collagen. These are the unprocessed images corresponding to CFP (donor excitation/donor emission; cyan), YPet (acceptor excitation/acceptor emission; yellow), and the sensitized emission FRET image (donor excitation/acceptor emission; purple).

A Z-stack of a human foreskin fibroblast (HFF) expressing GFP-AktPH (to detect PIP3, green) migrating in 3D 1.7 mg/ml collagen (reflection microscopy, red).

bioimagesFigure 3 - EImage 134067 (Figure 3 - A)Figure 3 - AImage 134062 (Figure 1 - B)Image 134075 (Figure 3 - E)Figure 3 - CImage 134071 (Figure 3 - C)Image 134064 (Figure 2 - D)Image 134068 (Figure 3 - A)Image 134072 (Figure 3 - C)Image 134069 (Figure 3 - A)Image 134076 (Figure 5 - E)Image 134065 (Figure 2 - D)Figure 1 - CFigure 1 - BImage 134073 (Figure 3 - E)Figure 5 - EFigure 5Image 134077 (Figure 5 - E)Image 134066 (Figure 2 - D)Figure 1Figure 2 - DImage 134063 (Figure 1 - C)Figure 3Image 134070 (Figure 3 - C)Figure 2Image 134074 (Figure 3 - E)Ryan J. PetrieNúria GavaraRichard S. ChadwickKenneth M. YamadafalseNonpolarized signaling reveals two distinct modes of 3D cell migration2012-04-30T11:23:13Z2018-11-29T11:23:13Z2018-11-29T11:23:13ZS-JCBD-20120112410.1083/jcb.201201124