bioimagesUnknownLaura Andrés-DelgadoThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201202137JCB

Conjugates formed by Jurkat cells with SEE-loaded Raji cells (as antigen presenting cells) were stained for Glu-MT (Alexa 594) shown in green and Tyr-MT (Alexa 647) shown in red. The raw images presented in the accompanying stack (left) were deconvolved using Huygens 3.0 software (Scientific Volume Imaging, Hilversum, The Netherlands).<br />The resulting stack of the deconvolved images is shown. These images were used to make the 3D reconstruction (Figure 2C and supplemental video).<br />

Jurkat cells were conjugated to latex beads coated with anti-TCR antibody for the indicated times. Cells were analyzed by immunoblotting for Glu-tubulin (55 kDa, upper part of the membrane) or for GAPDH (34 kDa, lower part of the membrane). Shown are two different exposures of the same membrane.

Conjugates formed by Jurkat cells with SEE-loaded Raji cells (as antigen presenting cells) were stained for Glu-MT (Alexa 594) shown in green and Tyr-MT (Alexa 647) shown in red. The images were analyzed under a confocal microscope (Zeiss LSM510). The stack of the raw images is shown.

bioimagesImage 136624 (Figure 2 - E)Image 136625 (Figure 2 - C)Figure 2 - EFigure 2 - CImage 137275 (Figure 2 - C)Figure 2Miguel A. AlonsoAna Ruiz-SáenzIsabel CorreasFrancesca BartoliniGregg G. GundersenLaura Andrés-DelgadoOlga M. AntónfalseINF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells2012-09-17T11:23:19Z2018-11-29T11:23:19Z2018-11-29T11:23:19ZS-JCBD-20120213710.1083/jcb.201202137