{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ana R.R. Maia"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201203091"],"attach_to":["JCB"],"legend":["HeLa H2B-mRFP (568, red) stable cells expressing GFP-CLASP2 wild-type (488, green) were imaged throughout mitosis.  There is normal mitosis in GFP-CLASP2 wild-type expressing cells.","GFP-CLASP2α (488, green) transiently expressed in HeLa cells and immunostained for Plk1 (568, red), ACA (647, purple) and DNA counterstained with DAPI (405, blue).  GFP-CLASP2 and Plk1 co-localize at centrosomes and kinetochores during prometaphase.","HeLa cells transfected with the dTOM20-CLASP2 construct (488, green) that drives the ectopic localization of CLASP2 to the mitochondria were immunostained for Plk1 (568, red) and the mitochondria counter-stained with MitoTracker (647, purple) and DNA with DAPI (405, blue). Ectopic expression of dTOM20-CLASP2 drives Plk1 recruitment to the mitochondria.","HeLa cell overexpressing GFP-CLASP2 S1234A mutant (488, green) immunostained for α-tubulin (568, red), ACA (647, purple) and DNA counterstained with DAPI (405, blue). S1234A overexpression results in monopolar spindles.","Image of an overexpressing GFP-CLASP2 S1234A HeLa cell (green, left) with an untransfected neighbour cell (right). Cells were immunostained for Plk1 (568, red), and DNA (405, blue).","HeLa H2B-mRFP (568, red) stable cells were transfected with GFP-CLASP2 S1225A mutant<br />(488, green) and mitotic progression was followed by spinning disk confocal microscopy.  The mitotic spindle collapses upon S1225A overexpression.","The movie shows GFP-CLASP2 S1225A mutant (488, green) and chromosomes are marked by mRFP-H2B (568, red). Mild expression of GFP-CLASP2 S1225A mutant causes a significant delay in mitosis and cells eventually enter anaphase in the presence of misaligned chromosomes. Later on, chromatin bridges are visible often resulting in cytokinesis failure."],"repository":["bioimages"],"figure_sub":["Image 133898 (Figure 2 - B)","Figure 5 - A","Figure 3 - H","Image 133897 (Figure 3 - A)","Video 1 - None","Image 133893 (Video 1 - None)","Figure 3 - A","Figure 5","Image 133894 (Video 4  - None)","Image 133854 (Figure 3 - H)","Video 3  - None","Video 4  - None","Video 1","Video 4","Video 3","Image 133899 (Video 3  - None)","Figure 3","Image 133907 (Figure 5 - A)","Figure 2","Figure 2 - B"],"pubmed_authors":["Zaira Garcia","Sandra Macedo-Ribeiro","Irina Kaverina","Helder Maiato","Stefano Maffini","Duane A. Compton","Iain M. Cheeseman","Marin Barisic","Lilian Kabeche","Ana R.R. Maia"],"additional_accession":[]},"is_claimable":false,"name":"Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments","description":null,"dates":{"release":"2012-10-08T11:23:23Z","modification":"2018-11-29T11:23:23Z","creation":"2018-11-29T11:23:23Z"},"accession":"S-JCBD-201203091","cross_references":{"doi":["10.1083/jcb.201203091"]}}