bioimagesUnknownAna R.R. MaiaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201203091JCB

HeLa H2B-mRFP (568, red) stable cells expressing GFP-CLASP2 wild-type (488, green) were imaged throughout mitosis. There is normal mitosis in GFP-CLASP2 wild-type expressing cells.

GFP-CLASP2α (488, green) transiently expressed in HeLa cells and immunostained for Plk1 (568, red), ACA (647, purple) and DNA counterstained with DAPI (405, blue). GFP-CLASP2 and Plk1 co-localize at centrosomes and kinetochores during prometaphase.

HeLa cells transfected with the dTOM20-CLASP2 construct (488, green) that drives the ectopic localization of CLASP2 to the mitochondria were immunostained for Plk1 (568, red) and the mitochondria counter-stained with MitoTracker (647, purple) and DNA with DAPI (405, blue). Ectopic expression of dTOM20-CLASP2 drives Plk1 recruitment to the mitochondria.

HeLa cell overexpressing GFP-CLASP2 S1234A mutant (488, green) immunostained for α-tubulin (568, red), ACA (647, purple) and DNA counterstained with DAPI (405, blue). S1234A overexpression results in monopolar spindles.

Image of an overexpressing GFP-CLASP2 S1234A HeLa cell (green, left) with an untransfected neighbour cell (right). Cells were immunostained for Plk1 (568, red), and DNA (405, blue).

HeLa H2B-mRFP (568, red) stable cells were transfected with GFP-CLASP2 S1225A mutant<br />(488, green) and mitotic progression was followed by spinning disk confocal microscopy. The mitotic spindle collapses upon S1225A overexpression.

The movie shows GFP-CLASP2 S1225A mutant (488, green) and chromosomes are marked by mRFP-H2B (568, red). Mild expression of GFP-CLASP2 S1225A mutant causes a significant delay in mitosis and cells eventually enter anaphase in the presence of misaligned chromosomes. Later on, chromatin bridges are visible often resulting in cytokinesis failure.

bioimagesImage 133898 (Figure 2 - B)Figure 5 - AFigure 3 - HImage 133897 (Figure 3 - A)Video 1 - NoneImage 133893 (Video 1 - None)Figure 3 - AFigure 5Image 133894 (Video 4 - None)Image 133854 (Figure 3 - H)Video 3 - NoneVideo 4 - NoneVideo 1Video 4Video 3Image 133899 (Video 3 - None)Figure 3Image 133907 (Figure 5 - A)Figure 2Figure 2 - BZaira GarciaSandra Macedo-RibeiroIrina KaverinaHelder MaiatoStefano MaffiniDuane A. ComptonIain M. CheesemanMarin BarisicLilian KabecheAna R.R. MaiafalseCdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments2012-10-08T11:23:23Z2018-11-29T11:23:23Z2018-11-29T11:23:23ZS-JCBD-20120309110.1083/jcb.201203091