bioimagesUnknownMaryna KapustinaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201204157JCBTransmission electron microscopy (TEM) image of the edge of a cell fixed during the oscillation. Samples were viewed using a LEO EM910 transmission electron microscope (Carl Zeiss SMT, Inc., Peabody, MA), operating at an accelerating voltage of 80 kV. Digital images were taken using a Gatan Orius SC 1000 CCD Camera and DigitalMicrograph 3.11.0 software.Time-lapse of Z stack imaging data of the fluorescent signal from F-actin (shown in greyscale) of an oscillating cell was recorded on the Zeiss Cell Observer Spinning Disk (Carl Zeiss AxioObserver Z1) with a Yokogawa CSU, Photometrics Evolve 512 x 512 camera, and stage top incubation. Images were recorded and processed using Axiovision version 4.8.2. software. One Z stack contains 80 planes at 0.5 µm intervals along the Z axis. Images inside the Z stack were recorded with 0.17 s intervalTime-lapse of Z stack imaging data from fluorescent signal arising from F-actin (GFP-Lifeact, in greyscale) in an oscillating cell was recorded on the Zeiss Cell Observer Spinning Disk (Carl Zeiss AxioObserver Z1) with a Yokogawa CSU, Photometrics Evolve 512 x 512 camera, and stage top incubation. Images were recorded and processed using Axiovision version 4.8.2. software. One Z stack contains 32 planes at 1µm intervals along the Z axis. Images inside the Z stack were recorded with 0.2 s interval.Transmission electron microscopy (TEM) image of cells spread for 24 hours on the cell culture dish. Samples were viewed using a LEO EM910 transmission electron microscope (Carl Zeiss SMT, Inc., Peabody, MA), operating at an accelerating voltage of 80 kV. Digital images were taken using a Gatan Orius SC 1000 CCD Camera and DigitalMicrograph 3.11.0 software.Time lapse of DIC and F-actin confocal fluorescent images of cells undergoing periodic protrusions. Images were acquired on the Olympus FluoView1000 laser confocal scanning microscope with an environmental chamber control using 60x oil immersion objective. Images were captured with 3.5 s intervalsTime lapse of DIC, F-actin (GFP-Lifeact, green) and plasma membrane (PMT-mRFP, red) of a non-oscillating rounded cell. Images were acquired on the Olympus FluoView1000 laser confocal scanning microscope with an environmental chamber control using 100x oil immersion objective. Images were captured with 7.2 s intervalsTransmission electron microscopy (TEM) image of a cell fixed during the oscillation. Samples were viewed using a LEO EM910 transmission electron microscope (Carl Zeiss SMT, Inc., Peabody, MA), operating at an accelerating voltage of 80 kV. Digital images were taken using a Gatan Orius SC 1000 CCD Camera and DigitalMicrograph 3.11.0 softwareTransmission electron microscopy (TEM) image of the cell fixed during the oscillation. Samples were viewed using a LEO EM910 transmission electron microscope (Carl Zeiss SMT, Inc., Peabody, MA), operating at an accelerating voltage of 80 kV. Digital images were taken using a Gatan Orius SC 1000 CCD Camera and DigitalMicrograph 3.11.0 software.Transmission electron microscopy (TEM) image of a cell fixed during the oscillation. Samples were viewed using a LEO EM910 transmission electron microscope (Carl Zeiss SMT, Inc., Peabody, MA), operating at an accelerating voltage of 80 kV. Digital images were taken using a Gatan Orius SC 1000 CCD Camera and DigitalMicrograph 3.11.0 software.bioimagesSupplemental Figure 4Image 134895 (Supplemental Figure 4 - b)Supplemental Figure 5Image 134891 (Figure 6 - a)Figure 5 - NoneImage 134888 (Figure 4 - a)Image 134892 (Figure 7 - a)Image 134896 (Supplemental Figure 4 - c)Supplemental Figure 4 - aSupplemental Figure 4 - bSupplemental Figure 4 - cSupplemental Figure 4 - dSupplemental Figure 4 - eFigure 6 - aSupplemental Figure 5 - cImage 134899 (Supplemental Figure 5 - c)Image 134897 (Supplemental Figure 4 - d)Image 134889 (Figure 4 - b)Figure 7 - aFigure 7 - bFigure 4 - aFigure 5Image 134898 (Supplemental Figure 4 - e)Figure 4Figure 4 - bImage 134890 (Figure 5 - None)Figure 7Figure 6Image 134894 (Supplemental Figure 4 - a)Image 134893 (Figure 7 - b)Maryna KapustinaTimothy C. ElstonKen JacobsonfalseCompression and dilation of the membrane-cortex layer generates rapid changes in cell shape2013-01-07T11:23:30Z2018-11-29T11:23:30Z2018-11-29T11:23:30ZS-JCBD-20120415710.1083/jcb.201204157