bioimagesUnknownNami SugiyamaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201205176JCB

MT1-MMP knockdown Hs578T cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

BT-549 cells were embedded in 3D type I collagen as a single cell suspension and allowed to grow for 4 days. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

BT-549 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2-D/I and MT1-MMP co-expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

EphA2 stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Shown is EphA2 labeling (green). DAPI (blue) visualizes nuclei.

EphA2-KD stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Shown is EphA2 labeling (green). DAPI (blue) visualizes nuclei.

MT1-MMP knockdown SUM159 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

MDA-MB-231 cells in 2D culture were incubated without PP2 (5μM) for 2 h (control). Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2-G/R stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with CD44 staining (green). DAPI (blue) visualizes nuclei.

EphA2 knockdown BT-549 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

SUM159 cells were embedded in 3D type I collagen as a single cell suspension and allowed to grow for 4 days. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

Hs578T cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

MT1-MMP knockdown BT-549 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Shown is EphA2 labeling (green). DAPI (blue) visualizes nuclei.

EphA2-KD-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Shown is EphA2 labeling (green). DAPI (blue) visualizes nuclei.

Hs578T cells were embedded in 3D type I collagen as a single cell suspension and allowed to grow for 4 days. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

MDA-MB-231 cells were plated in 2D culture. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2-KD-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with CD44 staining (green). DAPI (blue) visualizes nuclei.

MT1-MMP knockdown MDA-MB-231 cells were plated in 2D culture. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

SUM159 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2 knockdown MDA-MB-231 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2 knockdown SUM159 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2 stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with CD44 staining (green). DAPI (blue) visualizes nuclei.

EphA2D/I expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

EphA2 knockdown MDA-MB-231 cells were plated in 2D culture. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2-KD stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2 expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

EphA2-G/R and MT1-MMP co-expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

EphA2 knockdown Hs578T cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2 and MT1-MMP co-expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

EphA2 stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red). DAPI (blue) visualizes nuclei.

MDA-MB-231 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with CD44 staining (green). DAPI (blue) visualizes nuclei.

EphA2-KD-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2-KD stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with CD44 staining (green). DAPI (blue) visualizes nuclei.

EphA2-G/R stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red). DAPI (blue) visualizes nuclei.

MT1-MMP knockdown MDA-MB-231 cells were embedded within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red).

EphA2-G/R stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Shown is EphA2 labeling (green). DAPI (blue) visualizes nuclei.

EphA2-D/I stably overexpressing MDA-MB-231 cells were cultured within 3D collagen for 4 days. Morphology of colonies is visualized with filamentous actin staining (phalloidin; red). DAPI (blue) visualizes nuclei.

EphA2-G/R expressing MDA-MB-453 cells were cultured within 3D collagen for 6 days. Shown is labeling for EphA2 (green) and MT1-MMP (red). DAPI (blue) visualizes nuclei.

MDA-MB-231 cells were embedded in 3D type I collagen as a single cell suspension and allowed to grow for 4 days. Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

MDA-MB-231 cells in 2D culture were incubated with PP2 (5μM) for 2 h (PP2). Shown is labeling for EphA2 (green) and filamentous actin (phalloidin; red). DAPI (blue) visualizes nuclei.

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