{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Roberta Martinelli"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201209077"],"attach_to":["JCB"],"legend":["Live-cell imaging is shown of trans-cellular mechanical micro-wounding in MVEC transfected with actin-GFP (green) and membrane-DsRed (red). A single MVEC was subjected to 4 sequential mechanical micro-wounding events. Each time the initial wound rapidly expands as broken adhesions and cytoskeleton caused viscoelastic recoil of pre-existing tension. The MVEC then rapidly formed nodes of actin in a large ~20-30 micrometer radius around the pore with mixed VL and VW features. These exhibit directed propagation into the micro-wound to close it.","Live-cell imaging is shown of trans-cellular mechanical wounding in a micro-vascular endothelial cell co-expressing actin-GFP (green) and membrane-DsRed (red). Immediately following probe-mediated wounding, rapid cell retraction develops, followed by avid ventral lamellipodial recovery responses both specifically in orthogonally oriented directions (i.e., toward the upper and lower left corners). The non-responding regions opposite the major recoil/response areas (i.e., upper and lower left corners) are visible stretched and, therefore, tensed during wounding. The broken intracellular isometric tension is visibly transmitted to discrete regions of the unwounded neighbor cell (right) through intact adherens junctions that retract and translate toward the neighboring cell (toward the right).","Live-cell imaging is shown of lymphocytes (DIC images, blue) in the process of diapedesis across micro-vascular endothelial cells transfected with actin-GFP (green) and mDsRed (red). The field of view depicts a confluent endothelial monolayer in which only a single positive actin-GFP and mDsRed transfected cell is present. Immediately following completion of a trans-cellular diapedesis event (center of the cell), a burst of lamellar activity rapidly closes the trans-cellular pore and then continues to travel a significant distance beyond the distal pore edge before disappearing. A similar activity is seen during closure of para-cellular diapedesis gaps around the periphery of the cell."],"repository":["bioimages"],"figure_sub":["Figure 7","Figure 6","Figure 6 - b / Video 5 (Part I)","Figure 1 - b / Video 1 (Part I)","Image 248743 (Figure 6 - b / Video 5 (Part I))","Image 148292 (Figure 1 - b / Video 1 (Part I))","Figure 1","Image 248744 (Figure 7 - a / Video (Part I))","Figure 7 - a / Video (Part I)"],"pubmed_authors":["Tomas Kirchhausen","Timothy A. Springer","Ann M. Dvorak","Laya Varghese","Christopher V. Carman","Ramiro Massol","Tracey Sciuto","Peter T. Sage","Masataka Kamei","Roberta Martinelli","Mourad Toporsian"],"additional_accession":[]},"is_claimable":false,"name":"Release of cellular tension signals self-restorative ventral lamellipodia to heal barrier micro-wounds","description":null,"dates":{"release":"2013-04-29T11:23:55Z","modification":"2018-11-29T11:23:55Z","creation":"2018-11-29T11:23:55Z"},"accession":"S-JCBD-201209077","cross_references":{"doi":["10.1083/jcb.201209077"]}}