{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Rachel Allison"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201211045"],"attach_to":["JCB"],"legend":["GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin. Cells labelled with antibodies against myc (red) and GFP (green).","Confocal micrograph of HeLa cells subjected to spastin siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).  Gain settings have been adjusted to allow visualisation of the remaining transferrin receptor signal.","GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin. Cells were labelled with antibodies against myc (red) and GFP (green).","GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M1 spastin M87A. Cells were labelled with antibodies against  myc (red) and GFP (green).","Confocal micrograph of HeLa cells subjected to IST1 siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green). Gain settings have been adjusted to allow visualisation of the remaining transferrin receptor signal.","Confocal micrograph of HeLa cells subjected to spastin siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).","Confocal micrograph of HeLa cells subjected to IST1 siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).","GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin F124D.  Cells labelled with antibodies against myc (red) and GFP (green).","Confocal micrograph of HeLa cells subjected to mock siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green)."],"repository":["bioimages"],"figure_sub":["Image 291508 (Figure 7 - F)","Image 291513 (Figure 4 - F)","Figure 3 - A","Image 291512 (Figure 3 - B)","Figure 3 - B","Image 291505 (Figure 6 - F)","Figure 7 - F","Figure 7 - G","Figure 7 - H","Figure 4","Figure 7","Figure 6","Image 291509 (Figure 7 - G)","Image 291510 (Figure 7 - H)","Image 291507 (Figure 4 - G)","Image 291511 (Figure 3 - A)","Figure 4 - G","Figure 6 - G","Figure 4 - F","Figure 3","Figure 6 - E","Image 291504 (Figure 6 - E)","Image 291506 (Figure 6 - G)","Figure 6 - F"],"pubmed_authors":["Matthew N.J. Seaman","Evan Reid","Rachel Allison","James W. Connell","Coralie Fassier","Jamilé Hazan","Jennifer H. Lumb","Daniel Ten Martin"],"additional_accession":[]},"is_claimable":false,"name":"An ESCRT–spastin interaction promotes fission of recycling tubules from the endosome","description":null,"dates":{"release":"2013-07-29T11:24:04Z","modification":"2018-11-29T11:24:04Z","creation":"2018-11-29T11:24:04Z"},"accession":"S-JCBD-201211045","cross_references":{"doi":["10.1083/jcb.201211045"]}}