bioimagesUnknownRachel AllisonThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201211045JCB

GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin. Cells labelled with antibodies against myc (red) and GFP (green).

Confocal micrograph of HeLa cells subjected to spastin siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green). Gain settings have been adjusted to allow visualisation of the remaining transferrin receptor signal.

GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin. Cells were labelled with antibodies against myc (red) and GFP (green).

GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M1 spastin M87A. Cells were labelled with antibodies against myc (red) and GFP (green).

Confocal micrograph of HeLa cells subjected to IST1 siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green). Gain settings have been adjusted to allow visualisation of the remaining transferrin receptor signal.

Confocal micrograph of HeLa cells subjected to spastin siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).

Confocal micrograph of HeLa cells subjected to IST1 siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).

GFP-VPS4(E235Q) was transiently transfected into HeLa cell lines stably expressing myc-tagged M87 spastin F124D. Cells labelled with antibodies against myc (red) and GFP (green).

Confocal micrograph of HeLa cells subjected to mock siRNA transfection and labelled with antibodies to endogenous transferrin receptor (red) and mannose 6-phosphate receptor (green).

bioimagesImage 291508 (Figure 7 - F)Image 291513 (Figure 4 - F)Figure 3 - AImage 291512 (Figure 3 - B)Figure 3 - BImage 291505 (Figure 6 - F)Figure 7 - FFigure 7 - GFigure 7 - HFigure 4Figure 7Figure 6Image 291509 (Figure 7 - G)Image 291510 (Figure 7 - H)Image 291507 (Figure 4 - G)Image 291511 (Figure 3 - A)Figure 4 - GFigure 6 - GFigure 4 - FFigure 3Figure 6 - EImage 291504 (Figure 6 - E)Image 291506 (Figure 6 - G)Figure 6 - FMatthew N.J. SeamanEvan ReidRachel AllisonJames W. ConnellCoralie FassierJamilé HazanJennifer H. LumbDaniel Ten MartinfalseAn ESCRT–spastin interaction promotes fission of recycling tubules from the endosome2013-07-29T11:24:04Z2018-11-29T11:24:04Z2018-11-29T11:24:04ZS-JCBD-20121104510.1083/jcb.201211045