bioimagesUnknownNatalia A. BulgakovaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201211159JCB

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 3. Region 2

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 3. Region 1

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 1. Region 1<br /><br />

An overview of embryo 4 with visualized endogenous E-cad (anti-E-cad, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 1 with visualized endogenous Baz (anti-Baz, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 5 with visualized endogenous Baz (anti-Baz, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

A single confocal section through the region 2 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development. The same spots in embryo 6 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 2. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 2. Region 2

A time-lapse movie of E-cad-GFP (green) FRAP in cells that co-express baz-RNAi and CD8-Cherry (red) using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1.

Three consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1.

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 2

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 3 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 5. Region 1

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 2 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized MyoII-YFP protein trap (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 1. Region 3

A time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) and cells that co-express Baz-GFP and Shi-DN using en::Gal4 (green) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 4. Region 1

Four consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development.. The same spots in embryo 3 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A single confocal section through the region 1 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 4 with visualized endogenous Baz (anti-Baz, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 2

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 3. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 3. Region 2

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 4 with visualized endogenous E-cad (anti-E-cad, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

An overview of embryo 5 with visualized endogenous Baz (anti-Baz, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Stack of z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad) in control cells and cells expressing Spas using en::Gal4 in Drosophila embryonic epidermis at stage 15. Embryo 2

Stack of z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad) in control cells and cells expressing Spas using en::Gal4 in Drosophila embryonic epidermis at stage 15. Embryo 1

A time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized sqh::Rok-Venus (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 2 with visualized endogenous Baz (anti-Baz, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Three consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

A single confocal section through the region 2 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Three consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

An overview of embryo 3 with visualized endogenous Baz (anti-Baz, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

An overview of embryo 3 with visualized endogenous E-cad (anti-E-cad, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

A time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1.

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized sqh::Rok-Venus (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of 8 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) in the embryos that co-express Spastin, CD8-Cherry (red) and myr-GFP (green) with en::Gal4.

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 5. Region 1

Control embryos stained with anti-E-cad. Each image is a stack of 6 z-sections spaced by 0.38 µm.

Scans of blots using Li-Cor system. The lanes represent (left to right) input (3% of IP volume), co-IP with anti-E-cad; co-IP with anti-α-catenin, co-IP with anti-Baz; and co-IP with anti-GFP. The embryos expressing EB1-GFP under its own promoter were used for the experiment. Rabbit anti-GFP with anti-rabbit-700 secondary antibody were used to identify GFP protein in co-IP.

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development.. The same spots in embryo 5 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

A time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1.

A single confocal section through the region 1 of embryo 4 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 4. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized MyoII-YFP protein trap (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 4

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 5

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 6

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 1

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 2

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of first bleaching of 1µm circular region in E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching. Embryo 3

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

An overview of embryo 1 with visualized endogenous Baz (anti-Baz, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A single confocal section through the region 3 of embryo 1 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal sagital section through wild-type embryo stained with anti-E-cad (Cy3) and anti-Baz (Alexa-488).

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 2

A time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

A single confocal section through the region 2 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Five consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, grey) in Drosophila embryonic epidermis at stage 15. Embryo 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, grey) in Drosophila embryonic epidermis at stage 15. Embryo 1

An overview of embryo 5 with visualized endogenous E-cad (anti-E-cad, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A section with visualized endogenous Baz (anti-Baz, green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, grey) in Drosophila embryonic epidermis at stage 15. Embryo 4

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, grey) in Drosophila embryonic epidermis at stage 15. Embryo 3

An overview of embryo 1 with visualized endogenous E-cad (anti-E-cad, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, grey) in Drosophila embryonic epidermis at stage 15. Embryo 5

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A single confocal section through the region 1 of embryo 1 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

Stack of 8 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) in the embryos that co-express EB1-DN tagged with Cherry (red) and myr-GFP (green) with en::Gal4.

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 1. Region 1

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 2

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 1. Region 2

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

An overview of embryo 3 with visualized endogenous Baz (anti-Baz, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in a time-lapse moves with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

A single confocal section through the region 2 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development.. The same spots in embryo 2 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

A single confocal section through the region 2 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.<br />

Stack of 6 z-sections spaced by 0.38 µm with visualized MyoII-YFP protein trap (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

Stack of 8 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) in the embryos that co-express baz-RNAi, CD8-Cherry (red) and myr-GFP (green) with en::Gal4.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of E-cad-GFP (green) FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 2. Region 1

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 2. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2.

An overview of embryo 2 with visualized endogenous E-cad (anti-E-cad, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

Scans of blots using Li-Cor system. The lanes represent (left to right) input (3% of IP volume), co-IP with anti-E-cad; co-IP with anti-α-catenin, co-IP with anti-Baz; and co-IP with anti-GFP. The embryos expressing EB1-GFP under its own promoter were used for the experiment. Mouse anti-α-catenin with anti-mouse-800 secondary antibody were used to identify α-catenin protein in co-IP. (These data are not shown in the published figure.)

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 3. Region 1

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 3. Region 2

A single confocal section through the region 2 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 6 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 4 with visualized endogenous Baz (anti-Baz, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Stack of 6 z-sections spaced by 0.38 µm with visualized MyoII-YFP protein trap (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Three consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized sqh::Rok-Venus (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A time-lapse movie in epidermis at stage 15 of embryo development expressing EB1-GFP (black) and EB1-DN using en::Gal4. Frames were taken every 0.5 sec for 50 sec using spinning disc confocal microscope (Nikon Eclipse Ti-E inverted microscope, equipped with a CFI Apo TIRF100x 1.49 N.A. oil objective (Nikon) and a Yokogawa motorized CSU-X1-A1 confocal head). Embryo 1. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A single confocal section through the region 2 of embryo 2 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 2

An overview of embryo 2 with visualized endogenous E-cad (anti-E-cad, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Stack of 6 z-sections spaced by 0.38 µm with visualized sqh::Rok-Venus (green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, green) and cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 2. Region 2

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 2. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Stack of 8 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) in the embryos that co-express CD8-Cherry (red) and myr-GFP (green) with en::Gal4.

Control embryos stained with anti-β-catenin. Each image is a stack of 6 z-sections spaced by 0.38 µm.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

An overview of embryo 5 with visualized endogenous E-cad (anti-E-cad, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Stack of 6 z-sections spaced by 0.38 µm with visualized sqh::Rok-Venus (green) and cells that express CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 1. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

A time-lapse movie of E-cad-GFP FRAP in cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

Stack of z-sections spaced by 0.38 µm with visualized endogenous α-tubulin (anti-α-tubulin) in control cells and cells expressing Spas using en::Gal4 in Drosophila embryonic epidermis at stage 15. Embryo 1

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 1. Region 3

An overview of embryo 1 with visualized endogenous E-cad (anti-E-cad, red). Homozygosity is determined by absence of GFP fluorescence (green) from balancer chromosome.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, grey) in Drosophila embryonic epidermis at stage 15. Embryo 1

A single section of the same region in epidermis at stage 15 of embryo development expressing EB1-GFP and EB1-DN using en::Gal4. The cells that express EB1-DN are visualized in grey. Embryo 1. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, grey) in Drosophila embryonic epidermis at stage 15. Embryo 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, grey) in Drosophila embryonic epidermis at stage 15. Embryo 3

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, grey) in Drosophila embryonic epidermis at stage 15. Embryo 4

A time-lapse movie of E-cad-GFP FRAP in cells that co-express Shi-DN and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 2

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 3

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A single confocal section through the region 2 of embryo 1 used for ed-YFP protein trap FRAP experiment in time-lapse movie with ed-YFP (green) and cells that express EB1-DN tagged with Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, grey) in Drosophila embryonic epidermis at stage 15. Embryo 5

Four consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express Spastin and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that express CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 2

A single confocal section through the region 2 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Stack of z-sections spaced by 0.38 µm with visualized endogenous α-tubulin (anti-α-tubulin) in control cells and cells expressing Spas using en::Gal4 in Drosophila embryonic epidermis at stage 15. Embryo 2

A single confocal section through the region 1 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development.. The same spots in embryo 4 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express RhoGEF2 and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 2

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 1

A single confocal section through the region 1 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 1. Region 2

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous Baz (anti-Baz, green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 1 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Shi-DN and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 1

Scans of blots using Li-Cor system. The lanes represent (left to right) input (3% of IP volume), co-IP with anti-E-cad; co-IP with anti-α-catenin, co-IP with anti-Baz; and co-IP with anti-GFP. The embryos expressing EB1-GFP under its own promoter were used for the experiment. Rabbit anti-Baz with anti-rabbit-700 secondary antibody were used to identify Baz protein in co-IP.

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express Spastin and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Four consecutive parts of a time-lapse movie of E-cad-GFP recovery in control ubi::E-cad-GFP/+ embryos at stage 15 of development. In each embryo several circular regions of 0.5 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 3. Region 1

A single confocal section through the region 2 of embryo 3 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 1 of embryo 4 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express arm-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Two consecutive parts of a time-lapse movie of FRAP of E-cad-GFP in control ubi::E-cad-GFP/+ embryos at stage 15 of development.. The same spots in embryo 1 were rebleached 20 min after it was bleached for the first time. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total.

A time-lapse movie of ed-YFP protein trap FRAP in control cells and cells that express EB1-DN tagged with Cherry using en::Gal4 in Drosophila embryonic epidermis at stage 15. Stack of 6 z-sections spaced by 0.38 µm that was imaged just before photobleaching, immediately after photobleaching, and then at 10 sec intervals. The average projection of the first z-stack (before photobleaching) was used in Fig.2a and for measuring ed protein levels. Embryo 4. Region 1

An overview of embryo 2 with visualized endogenous Baz (anti-Baz, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Two consecutive parts of a time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 4. Region 1

Stack of 6 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, green) and cells that co-express arm-RNAi with CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

Three consecutive parts of a time-lapse movie of E-cad-GFP FRAP in eb1 mutant embryos at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1

A time-lapse movie of FRAP of Baz-GFP expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 2 µm diameter were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 5. Region 2

An overview of embryo 3 with visualized endogenous E-cad (anti-E-cad, red). Heterozygosity for eb1 mutant allele is determined by presence of GFP fluorescence (green) from balancer chromosome that carries wild type EB1 allele.

Stack of 8 z-sections spaced by 0.38 µm with visualized endogenous E-cad (anti-E-cad, red) in the embryos that express myr-GFP (green) with en::Gal4.

A time-lapse movie of E-cad-GFP FRAP in a control ubi::E-cad-GFP/+ embryo at stage 15 of development. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 6. Region 2.

A single confocal section through the region 1 of embryo 5 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express RhoGEF2-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

A single confocal section through the region 2 of embryo 2 used for E-cad-GFP FRAP experiment in time-lapse movie with E-cad-GFP (green) and cells that co-express baz-RNAi and CD8-Cherry using en::Gal4 (red) in Drosophila embryonic epidermis at stage 15.

bioimagesImage 250265 (Supplemental Figure 2 - E,F,G,H)Image 248931 (Figure 6 - A,B. Spastin)Image 249512 (Figure 2 - C,D)Image 249922 (Figure 6 - E,F,G,H. Spastin)Image 249975 (Figure 7 - E,F,G,H. RhoGEF2)Image 249127 (Supplemental Figure 1 - A,B)Image 250321 (Figure 9 - A,B. Spastin)Image 249827 (Figure 4 - C,D)Image 250154 (Figure 9 - A,B. CD8)Image 249021 (Figure 8 - A,B. CD8)Image 248894 (Figure 4 - E)Image 249601 (Figure 2 - G,H)Image 250028 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 248942 (Figure 6 - C,D. EB1-DN)Image 249665 (Figure 3 - I. baz-RNAi)Image 249553 (Figure 2 - E,F)Image 250386 (Figure 9 - A,B. baz-RNAi)Image 249737 (Figure 3 - G,I. CD8)Image 250395 (Figure 9 - A,B. baz-RNAi)Image 249162 (Supplemental Figure 2 - A,B. Control)Image 248982 (Figure 7 - A,B. RhoGEF2-RNAi)Image 250253 (Supplemental Figure 2 - E,F,G,H)Image 249786 (Figure 4 - A. 1 µm)Image 249829 (Figure 4 - C,D)Image 249860 (Figure 6 - E,F,G,H. EB1-DN)Image 250275 (Supplemental Figure 2 - E,F,G,H)Image 250299 (Figure 9 - A,B. Spastin)Figure 6 - A,B. EB1-DNImage 249173 (Supplemental Figure 2 - C,D. Control)Image 250390 (Figure 9 - A,B. baz-RNAi)Image 250161 (Figure 9 - A,B. CD8)Supplemental Figure 2 - A,B. ControlImage 250381 (Figure 9 - A,B. baz-RNAi)Image 249825 (Figure 4 - C,D)Image 250349 (Figure 2 - A,B)Image 250122 (Figure 9 - A,B. CD8)Image 249637 (Figure 3 - G. baz-RNAi)Image 250414 (Figure 9 - A,B. en)Image 250409 (Figure 9 - A,B. en)Image 250263 (Supplemental Figure 2 - E,F,G,H)Image 248908 (Figure 5 - C,D,E,F,G,H,I)Image 250302 (Figure 9 - A,B. Spastin)Image 249109 (Figure 9 - D,E. Spastin)Image 249569 (Figure 2 - G,H)Image 248998 (Figure 7 - C,D. RhoGEF2-RNAi)Image 249002 (Figure 7 - C,D. RhoGEF2-RNAi)Image 250118 (Figure 9 - A,B. CD8)Image 249510 (Figure 2 - C,D)Image 250255 (Supplemental Figure 2 - E,F,G,H)Image 249567 (Figure 2 - G,H)Image 248841 (Figure 3 - D,F)Image 250427 (Figure 9 - A,B. en)Image 249100 (Figure 9 - D,E. EB1-DN)Image 250347 (Figure 2 - A,B)Image 248924 (Figure 6 - A,B. Spastin)Image 249058 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250273 (Supplemental Figure 2 - E,F,G,H)Image 248799 (Figure 1 - F,G)Image 248896 (Figure 5 - C,D,E,F,G,H,I)Image 249875 (Figure 6 - E,F,G,H. EB1-DN)Image 248912 (Figure 5 - C,D,E,F,G,H,I)Image 249867 (Figure 6 - E,F,G,H. EB1-DN)Image 249169 (Supplemental Figure 2 - C,D. Control)Image 249880 (Figure 6 - E,F,G,H. EB1-DN)Image 250214 (Figure 9 - A,B. EB1-DN)Image 249969 (Figure 7 - E,F,G,H. RhoGEF2)Image 249140 (Supplemental Figure 2 - A,B. eb1)Image 250042 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250293 (Figure 9 - A,B. Spastin)Image 248772 (Figure 1 - D,E)Image 249798 (Figure 4 - A. 2 µm)Image 250314 (Figure 9 - A,B. Spastin)Image 249520 (Figure 2 - E,F)Image 248973 (Figure 7 - A,B. RhoGEF2)Image 250257 (Supplemental Figure 2 - E,F,G,H)Image 250081 (Figure 9 - A,B. CD8)Image 250368 (Figure 9 - A,B. baz-RNAi)Image 249920 (Figure 6 - E,F,G,H. Spastin)Image 249821 (Figure 4 - C,D)Image 250377 (Figure 9 - A,B. baz-RNAi)Image 249973 (Figure 7 - E,F,G,H. RhoGEF2)Image 248946 (Figure 6 - C,D. Spastin)Image 250100 (Figure 9 - A,B. CD8)Image 249605 (Figure 2 - G,H)Image 250115 (Figure 9 - A,B. CD8)Image 250431 (Figure 9 - A,B. en)Image 249628 (Figure 3 - G. baz-RNAi)Image 250157 (Figure 9 - A,B. CD8)Image 249006 (Figure 7 - C,D. RhoGEF2-RNAi)Image 249065 (Figure 8 - A,B. Spastin)Image 250271 (Supplemental Figure 2 - E,F,G,H)Image 249945 (Figure 6 - E,F,G,H. EB1-DN)Image 248845 (Figure 3 - D,F)Image 250426 (Figure 9 - A,B. en)Image 249126 (Supplemental Figure 1 - A,B)Image 250040 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250099 (Figure 9 - A,B. CD8)Image 250231 (Figure 9 - A,B. EB1-DN)Image 249708 (Figure 3 - G,I. CD8)Image 250207 (Figure 9 - A,B. EB1-DN)Image 250356 (Figure 9 - A,B. baz-RNAi)Image 249116 (Figure 9 - D,E. Spastin)Image 249751 (Figure 3 - G,I. CD8)Image 249977 (Figure 7 - E,F,G,H. RhoGEF2)Image 249046 (Figure 8 - A,B. RhoGEF2)Image 249734 (Figure 3 - G,I. CD8)Image 250206 (Figure 9 - A,B. EB1-DN)Image 250221 (Figure 9 - A,B. EB1-DN)Image 249696 (Figure 3 - G,I. CD8)Image 250251 (Supplemental Figure 2 - E,F,G,H)Image 250216 (Figure 9 - A,B. EB1-DN)Image 250209 (Figure 9 - A,B. EB1-DN)Image 250222 (Figure 9 - A,B. EB1-DN)Image 249096 (Figure 9 - D,E. CD8)Image 249124 (Supplemental Figure 1 - A,B)Image 250277 (Supplemental Figure 2 - E,F,G,H)Image 249790 (Figure 4 - A. 2 µm)Supplemental Figure 1 - C,D,E,FImage 248988 (Figure 7 - C,D. RhoGEF2)Figure 7 - E,F,G,H. RhoGEF2Image 249747 (Figure 3 - G,I. CD8)Image 249154 (Supplemental Figure 2 - A,B. Control)Image 249181 (Supplemental Figure 2 - C,D. Control)Image 250223 (Figure 9 - A,B. EB1-DN)Image 250215 (Figure 9 - A,B. EB1-DN)Image 250269 (Supplemental Figure 2 - E,F,G,H)Image 250230 (Figure 9 - A,B. EB1-DN)Image 250327 (Figure 9 - A,B. Spastin)Image 249125 (Supplemental Figure 1 - A,B)Image 250208 (Figure 9 - A,B. EB1-DN)Image 249712 (Figure 3 - G,I. CD8)Image 249091 (Figure 9 - D,E. CD8)Image 249084 (Figure 9 - D,E. baz-RNAi)Figure 9 - A,B. CD8Image 248925 (Figure 6 - A,B. Spastin)Image 249688 (Figure 1 - F,G)Image 250128 (Figure 9 - A,B. CD8)Image 250286 (Figure 9 - A,B. Spastin)Image 250103 (Figure 9 - A,B. CD8)Image 248987 (Figure 7 - A,B. RhoGEF2-RNAi)Image 249036 (Figure 8 - A,B. EB1-DN)Image 248830 (Figure 3 - C,E)Image 249029 (Figure 8 - A,B. CD8)Image 250365 (Figure 9 - A,B. baz-RNAi)Image 248920 (Figure 6 - A,B. EB1-DN)Image 248945 (Figure 6 - C,D. Spastin)Image 250084 (Figure 9 - A,B. CD8)Image 248898 (Figure 5 - C,D,E,F,G,H,I)Image 250294 (Figure 9 - A,B. Spastin)Image 249915 (Figure 6 - E,F,G,H. Spastin)Image 250190 (Supplemental Figure 1 - C,D,E,F)Figure 1 - A,BImage 249839 (Figure 4 - C,D)Figure 8 - A,B. SpastinImage 249702 (Figure 3 - G,I. CD8)Image 250326 (Figure 9 - A,B. Spastin)Image 249663 (Figure 3 - I. baz-RNAi)Image 249514 (Figure 2 - E,F)Figure 5 - C,D,E,F,G,H,IFigure 3 - I. baz-RNAiImage 248933 (Figure 6 - C,D. EB1-DN)Image 250430 (Figure 9 - A,B. en)Image 248929 (Figure 6 - A,B. Spastin)Image 249983 (Figure 7 - E,F,G,H. RhoGEF2)Image 248804 (Figure 3 - B)Image 250319 (Figure 9 - A,B. Spastin)Image 249809 (Figure 4 - A. 2 µm)Image 248906 (Figure 5 - C,D,E,F,G,H,I)Image 250281 (Supplemental Figure 2 - E,F,G,H)Image 249153 (Supplemental Figure 2 - A,B. Control)Image 250044 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250303 (Figure 9 - A,B. Spastin)Image 249638 (Figure 3 - G. baz-RNAi)Image 249142 (Supplemental Figure 2 - A,B. eb1)Image 250110 (Figure 9 - A,B. CD8)Image 249800 (Figure 4 - A. 2 µm)Image 248829 (Figure 3 - C,E)Image 249500 (Figure 2 - C,D)Image 249577 (Figure 2 - G,H)Image 249087 (Figure 9 - D,E. CD8)Image 249981 (Figure 7 - E,F,G,H. RhoGEF2)Image 250144 (Figure 9 - A,B. CD8)Image 249119 (Supplemental Figure 1 - A,B)Image 249849 (Figure 6 - E,F,G,H. EB1-DN)Image 249082 (Figure 9 - D,E. baz-RNAi)Image 250079 (Figure 9 - A,B. CD8)Image 249807 (Figure 4 - A. 2 µm)Image 249141 (Supplemental Figure 2 - A,B. eb1)Figure 4 - C,DImage 249768 (Figure 4 - A. 1 µm)Image 248930 (Figure 6 - A,B. Spastin)Image 249862 (Figure 6 - E,F,G,H. EB1-DN)Image 250132 (Figure 9 - A,B. CD8)Image 249913 (Figure 6 - E,F,G,H. Spastin)Image 249069 (Figure 8 - A,B. Spastin)Image 250166 (Figure 9 - A,B. CD8)Image 250320 (Figure 9 - A,B. Spastin)Image 248941 (Figure 6 - C,D. EB1-DN)Figure 6 - A,B. SpastinImage 249757 (Figure 3 - G,I. CD8)Figure 7 - A,B. RhoGEF2-RNAiImage 249724 (Figure 3 - G,I. CD8)Image 249675 (Figure 3 - I. baz-RNAi)Image 249163 (Supplemental Figure 2 - A,B. Control)Image 249102 (Figure 9 - D,E. EB1-DN)Image 249042 (Figure 8 - A,B. RhoGEF2)Image 248843 (Figure 3 - D,F)Image 249054 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250287 (Figure 9 - A,B. Spastin)Image 250009 (Figure 7 - E,F,G,H. RhoGEF2)Image 250167 (Supplemental Figure 1 - C,D,E,F)Image 249607 (Figure 2 - G,H)Image 250383 (Figure 9 - A,B. baz-RNAi)Image 250259 (Supplemental Figure 2 - E,F,G,H)Image 248801 (Figure 3 - A)Image 250363 (Figure 9 - A,B. baz-RNAi)Image 250091 (Figure 9 - A,B. CD8)Image 249819 (Figure 4 - C,D)Image 250024 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249177 (Supplemental Figure 2 - C,D. Control)Image 250301 (Figure 9 - A,B. Spastin)Image 250048 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249146 (Supplemental Figure 2 - A,B. eb1)Image 249657 (Figure 3 - I. baz-RNAi)Image 249071 (Figure 8 - A,B. Spastin)Image 249792 (Figure 4 - A. 2 µm)Figure 6 - C,D. EB1-DNImage 250147 (Figure 9 - A,B. CD8)Image 249917 (Figure 6 - E,F,G,H. Spastin)Image 250397 (Figure 9 - A,B. baz-RNAi)Image 250005 (Figure 7 - E,F,G,H. RhoGEF2)Image 250249 (Figure 9 - A,B. EB1-DN)Image 248935 (Figure 6 - C,D. EB1-DN)Image 249506 (Figure 2 - C,D)Image 249683 (Figure 3 - I. baz-RNAi)Image 250087 (Figure 9 - A,B. CD8)Image 250413 (Figure 9 - A,B. en)Image 249694 (Figure 1 - F,G)Image 249659 (Figure 3 - I. baz-RNAi)Image 250125 (Figure 9 - A,B. CD8)Image 250428 (Figure 9 - A,B. en)Image 249561 (Figure 2 - E,F)Image 250443 (Figure 2 - A,B)Image 250151 (Figure 9 - A,B. CD8)Image 249761 (Figure 3 - G,I. CD8)Image 249472 (Figure 1 - F,G)Image 249541 (Figure 2 - E,F)Figure 9 - A,B. baz-RNAiImage 249178 (Supplemental Figure 2 - C,D. Control)Image 249673 (Figure 3 - I. baz-RNAi)Image 250322 (Figure 9 - A,B. Spastin)Image 249038 (Figure 8 - A,B. EB1-DN)Image 248937 (Figure 6 - C,D. EB1-DN)Image 249478 (Figure 1 - F,G)Image 249528 (Figure 2 - E,F)Image 249681 (Figure 3 - I. baz-RNAi)Image 249918 (Figure 6 - E,F,G,H. Spastin)Image 248981 (Figure 7 - A,B. RhoGEF2-RNAi)Image 250007 (Figure 7 - E,F,G,H. RhoGEF2)Image 248968 (Figure 7 - A,B. RhoGEF2)Image 248773 (Figure 1 - D,E)Image 248938 (Figure 6 - C,D. EB1-DN)Image 250186 (Supplemental Figure 1 - C,D,E,F)Image 249003 (Figure 7 - C,D. RhoGEF2-RNAi)Image 248764 (Figure 1 - A,B)Image 250313 (Figure 9 - A,B. Spastin)Image 249654 (Figure 3 - I. baz-RNAi)Image 249753 (Figure 3 - G,I. CD8)Image 248951 (Figure 6 - C,D. Spastin)Image 250370 (Figure 9 - A,B. baz-RNAi)Image 248899 (Figure 5 - C,D,E,F,G,H,I)Image 249186 (Supplemental Figure 2 - C,D. eb1)Image 249985 (Figure 7 - E,F,G,H. RhoGEF2)Image 248905 (Figure 5 - C,D,E,F,G,H,I)Image 249990 (Figure 7 - E,F,G,H. RhoGEF2)Figure 6 - E,F,G,H. EB1-DNImage 248972 (Figure 7 - A,B. RhoGEF2)Image 250233 (Figure 9 - A,B. EB1-DN)Image 250198 (Supplemental Figure 1 - C,D,E,F)Image 248836 (Figure 3 - D,F)Image 249120 (Supplemental Figure 1 - A,B)Image 250058 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250127 (Figure 9 - A,B. CD8)Image 250328 (Figure 9 - A,B. Spastin)Image 249034 (Figure 8 - A,B. EB1-DN)Image 249986 (Figure 7 - E,F,G,H. RhoGEF2)Image 250200 (Supplemental Figure 1 - C,D,E,F)Image 249185 (Supplemental Figure 2 - C,D. eb1)Image 248999 (Figure 7 - C,D. RhoGEF2-RNAi)Image 249121 (Supplemental Figure 1 - A,B)Image 249149 (Supplemental Figure 2 - A,B. eb1)Image 250226 (Figure 9 - A,B. EB1-DN)Image 249710 (Figure 3 - G,I. CD8)Image 249873 (Figure 6 - E,F,G,H. EB1-DN)Image 249148 (Supplemental Figure 2 - A,B. eb1)Figure 4 - EImage 250064 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Figure 7 - A,B. RhoGEF2Image 250188 (Supplemental Figure 1 - C,D,E,F)Image 249482 (Figure 1 - F,G)Image 248859 (Figure 2 - C,D)Image 249833 (Figure 4 - C,D)Image 250156 (Figure 9 - A,B. CD8)Image 249193 (Supplemental Figure 2 - C,D. eb1)Image 249174 (Supplemental Figure 2 - C,D. Control)Image 249661 (Figure 3 - I. baz-RNAi)Image 250325 (Figure 9 - A,B. Spastin)Image 249589 (Figure 2 - G,H)Image 249063 (Figure 8 - A,B. Spastin)Image 250083 (Figure 9 - A,B. CD8)Image 249108 (Figure 9 - D,E. Spastin)Image 249059 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250131 (Figure 9 - A,B. CD8)Image 249888 (Figure 6 - E,F,G,H. EB1-DN)Figure 3Figure 2Image 249587 (Figure 2 - G,H)Image 250196 (Supplemental Figure 1 - C,D,E,F)Image 249911 (Figure 6 - E,F,G,H. Spastin)Image 249168 (Supplemental Figure 2 - C,D. Control)Image 249147 (Supplemental Figure 2 - A,B. eb1)Image 250358 (Figure 9 - A,B. baz-RNAi)Image 249899 (Figure 6 - E,F,G,H. Spastin)Image 250412 (Figure 9 - A,B. en)Image 250429 (Figure 9 - A,B. en)Image 249547 (Figure 2 - E,F)Image 249728 (Figure 3 - G,I. CD8)Image 248922 (Figure 6 - A,B. EB1-DN)Image 250202 (Supplemental Figure 1 - C,D,E,F)Image 249611 (Figure 3 - G. baz-RNAi)Image 250183 (Supplemental Figure 1 - C,D,E,F)Image 249194 (Supplemental Figure 2 - C,D. eb1)Figure 9Image 250353 (Figure 9 - A,B. baz-RNAi)Figure 8Figure 7Figure 1Figure 6Figure 5Figure 4Image 249504 (Figure 2 - C,D)Image 249835 (Figure 4 - C,D)Image 249612 (Figure 3 - G. baz-RNAi)Image 249122 (Supplemental Figure 1 - A,B)Image 250090 (Figure 9 - A,B. CD8)Image 250141 (Figure 9 - A,B. CD8)Image 249878 (Figure 6 - E,F,G,H. EB1-DN)Image 250124 (Figure 9 - A,B. CD8)Image 249897 (Figure 6 - E,F,G,H. Spastin)Image 249732 (Figure 3 - G,I. CD8)Image 249498 (Figure 2 - C,D)Figure 8 - A,B. RhoGEF2Image 249619 (Figure 3 - G. baz-RNAi)Image 250178 (Supplemental Figure 1 - C,D,E,F)Image 249853 (Figure 6 - E,F,G,H. EB1-DN)Image 250205 (Supplemental Figure 1 - C,D,E,F)Image 249560 (Figure 2 - E,F)Image 250193 (Supplemental Figure 1 - C,D,E,F)Image 248985 (Figure 7 - A,B. RhoGEF2-RNAi)Image 249188 (Supplemental Figure 2 - C,D. eb1)Image 249959 (Figure 7 - E,F,G,H. RhoGEF2)Image 249094 (Figure 9 - D,E. CD8)Image 249706 (Figure 3 - G,I. CD8)Image 250227 (Figure 9 - A,B. EB1-DN)Image 249166 (Supplemental Figure 2 - A,B. Control)Image 249585 (Figure 2 - G,H)Image 249484 (Figure 1 - F,G)Image 250389 (Figure 9 - A,B. baz-RNAi)Image 249749 (Figure 3 - G,I. CD8)Image 250212 (Figure 9 - A,B. EB1-DN)Image 248975 (Figure 7 - A,B. RhoGEF2)Supplemental Figure 2 - C,D. eb1Image 250357 (Figure 9 - A,B. baz-RNAi)Image 248778 (Figure 1 - D,E)Image 250366 (Figure 9 - A,B. baz-RNAi)Image 250159 (Figure 9 - A,B. CD8)Image 250442 (Figure 9 - A,B. en)Image 249196 (Supplemental Figure 2 - C,D. eb1)Image 250232 (Figure 9 - A,B. EB1-DN)Image 250217 (Figure 9 - A,B. EB1-DN)Image 250169 (Supplemental Figure 1 - C,D,E,F)Image 249123 (Supplemental Figure 1 - A,B)Image 249543 (Figure 2 - E,F)Figure 3 - D,FImage 248989 (Figure 7 - C,D. RhoGEF2)Image 250072 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249893 (Figure 6 - E,F,G,H. EB1-DN)Image 250438 (Figure 9 - A,B. en)Image 248910 (Figure 5 - C,D,E,F,G,H,I)Image 250163 (Figure 9 - A,B. CD8)Image 250398 (Figure 9 - A,B. en)Figure 5 - AImage 249066 (Figure 8 - A,B. Spastin)Image 250102 (Figure 9 - A,B. CD8)Image 250403 (Figure 9 - A,B. en)Image 249625 (Figure 3 - G. baz-RNAi)Image 250086 (Figure 9 - A,B. CD8)Image 250316 (Figure 9 - A,B. Spastin)Image 249949 (Figure 6 - E,F,G,H. EB1-DN)Figure 7 - C,D. RhoGEF2-RNAiImage 250246 (Figure 9 - A,B. EB1-DN)Image 249999 (Figure 7 - E,F,G,H. RhoGEF2)Image 249538 (Figure 2 - E,F)Image 250213 (Figure 9 - A,B. EB1-DN)Image 248974 (Figure 7 - A,B. RhoGEF2)Image 249843 (Figure 4 - C,D)Image 249579 (Figure 2 - G,H)Image 250417 (Figure 9 - A,B. en)Image 250137 (Figure 9 - A,B. CD8)Image 249772 (Figure 4 - A. 1 µm)Image 249951 (Figure 7 - E,F,G,H. RhoGEF2)Image 248909 (Figure 5 - C,D,E,F,G,H,I)Image 249904 (Figure 6 - E,F,G,H. Spastin)Image 250112 (Figure 9 - A,B. CD8)Image 249841 (Figure 4 - C,D)Image 250146 (Figure 9 - A,B. CD8)Image 249718 (Figure 3 - G,I. CD8)Image 249763 (Figure 3 - G,I. CD8)Image 249648 (Figure 3 - I. baz-RNAi)Image 249895 (Figure 6 - E,F,G,H. Spastin)Image 250354 (Figure 9 - A,B. baz-RNAi)Image 250315 (Figure 9 - A,B. Spastin)Image 249635 (Figure 3 - G. baz-RNAi)Image 249678 (Figure 3 - I. baz-RNAi)Image 250415 (Figure 9 - A,B. en)Image 248940 (Figure 6 - C,D. EB1-DN)Image 249474 (Figure 1 - F,G)Image 250153 (Figure 9 - A,B. CD8)Image 248769 (Figure 1 - D,E)Image 250408 (Figure 9 - A,B. en)Image 250011 (Figure 7 - E,F,G,H. RhoGEF2)Image 250242 (Figure 9 - A,B. EB1-DN)Figure 9 - A,B. EB1-DNImage 250191 (Supplemental Figure 1 - C,D,E,F)Image 250330 (Figure 2 - A,B)Image 249992 (Figure 7 - E,F,G,H. RhoGEF2)Figure 9 - D,E. CD8Image 248913 (Figure 6 - A,B. EB1-DN)Image 249064 (Figure 8 - A,B. Spastin)Image 250105 (Figure 9 - A,B. CD8)Image 250160 (Figure 9 - A,B. CD8)Image 249890 (Figure 6 - E,F,G,H. EB1-DN)Image 249837 (Figure 4 - C,D)Image 249690 (Figure 1 - F,G)Image 249024 (Figure 8 - A,B. CD8)Image 249539 (Figure 2 - E,F)Image 249007 (Figure 7 - C,D. RhoGEF2-RNAi)Image 248977 (Figure 7 - A,B. RhoGEF2)Image 250013 (Figure 7 - E,F,G,H. RhoGEF2)Image 249101 (Figure 9 - D,E. EB1-DN)Image 248893 (Figure 5 - A)Image 248901 (Figure 5 - C,D,E,F,G,H,I)Image 250093 (Figure 9 - A,B. CD8)Image 250382 (Figure 9 - A,B. baz-RNAi)Image 249994 (Figure 7 - E,F,G,H. RhoGEF2)Image 250394 (Figure 9 - A,B. baz-RNAi)Image 249171 (Supplemental Figure 2 - C,D. Control)Image 249502 (Figure 2 - C,D)Image 250074 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249537 (Figure 2 - E,F)Image 249774 (Figure 4 - A. 1 µm)Image 249858 (Figure 6 - E,F,G,H. EB1-DN)Supplemental Figure 2 - E,F,G,HImage 249545 (Figure 2 - E,F)Image 250019 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249741 (Figure 3 - G,I. CD8)Image 249050 (Figure 8 - A,B. RhoGEF2)Image 250410 (Figure 9 - A,B. en)Image 250433 (Figure 9 - A,B. en)Image 249909 (Figure 6 - E,F,G,H. Spastin)Image 249172 (Supplemental Figure 2 - C,D. Control)Image 250066 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250089 (Figure 9 - A,B. CD8)Image 250149 (Figure 9 - A,B. CD8)Image 249097 (Figure 9 - D,E. EB1-DN)Figure 4 - A. 1 µmImage 248952 (Figure 6 - C,D. Spastin)Image 250424 (Figure 9 - A,B. en)Image 249033 (Figure 8 - A,B. EB1-DN)Image 249195 (Supplemental Figure 2 - C,D. eb1)Image 250108 (Figure 9 - A,B. CD8)Image 250279 (Supplemental Figure 2 - E,F,G,H)Image 249062 (Figure 8 - A,B. Spastin)Image 248803 (Figure 3 - B)Image 250096 (Figure 9 - A,B. CD8)Image 250134 (Figure 9 - A,B. CD8)Image 249670 (Figure 3 - I. baz-RNAi)Image 249902 (Figure 6 - E,F,G,H. Spastin)Image 250312 (Figure 9 - A,B. Spastin)Image 250026 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249759 (Figure 3 - G,I. CD8)Image 249668 (Figure 3 - I. baz-RNAi)Image 249187 (Supplemental Figure 2 - C,D. eb1)Image 250267 (Supplemental Figure 2 - E,F,G,H)Image 249788 (Figure 4 - A. 1 µm)Image 249027 (Figure 8 - A,B. CD8)Image 248934 (Figure 6 - C,D. EB1-DN)Image 249722 (Figure 3 - G,I. CD8)Image 250310 (Figure 9 - A,B. Spastin)Image 248980 (Figure 7 - A,B. RhoGEF2-RNAi)Image 250374 (Figure 9 - A,B. baz-RNAi)Image 248840 (Figure 3 - D,F)Image 250401 (Figure 9 - A,B. en)Image 249060 (Figure 8 - A,B. RhoGEF2-RNAi)Image 249700 (Figure 3 - G,I. CD8)Image 249105 (Figure 9 - D,E. EB1-DN)Image 248865 (Figure 2 - C,D)Image 249037 (Figure 8 - A,B. EB1-DN)Image 249680 (Figure 3 - I. baz-RNAi)Image 249686 (Figure 1 - F,G)Image 249031 (Figure 8 - A,B. CD8)Image 250311 (Figure 9 - A,B. Spastin)Image 249055 (Figure 8 - A,B. RhoGEF2-RNAi)Image 248953 (Figure 6 - C,D. Spastin)Image 249078 (Figure 9 - D,E. baz-RNAi)Image 250362 (Figure 9 - A,B. baz-RNAi)Image 249051 (Figure 8 - A,B. RhoGEF2)Image 248765 (Figure 1 - A,B)Image 249906 (Figure 6 - E,F,G,H. Spastin)Image 249079 (Figure 9 - D,E. baz-RNAi)Image 250346 (Figure 2 - A,B)Image 250085 (Figure 9 - A,B. CD8)Image 248943 (Figure 6 - C,D. Spastin)Image 249996 (Figure 7 - E,F,G,H. RhoGEF2)Image 249525 (Figure 2 - E,F)Image 249643 (Figure 3 - I. baz-RNAi)Image 250355 (Figure 9 - A,B. baz-RNAi)Image 250405 (Figure 9 - A,B. en)Image 249061 (Figure 8 - A,B. RhoGEF2-RNAi)Image 248992 (Figure 7 - C,D. RhoGEF2)Image 250418 (Figure 9 - A,B. en)Image 249593 (Figure 2 - G,H)Image 250440 (Figure 9 - A,B. en)Image 248918 (Figure 6 - A,B. EB1-DN)Image 249056 (Figure 8 - A,B. RhoGEF2-RNAi)Image 249554 (Figure 2 - E,F)Image 249866 (Figure 6 - E,F,G,H. EB1-DN)Image 250034 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250104 (Figure 9 - A,B. CD8)Image 250344 (Figure 2 - A,B)Image 250317 (Figure 9 - A,B. Spastin)Image 249156 (Supplemental Figure 2 - A,B. Control)Image 249622 (Figure 3 - G. baz-RNAi)Image 249083 (Figure 9 - D,E. baz-RNAi)Image 248777 (Figure 1 - D,E)Image 249595 (Figure 2 - G,H)Image 250296 (Figure 9 - A,B. Spastin)Image 249876 (Figure 6 - E,F,G,H. EB1-DN)Image 249692 (Figure 1 - F,G)Image 250111 (Figure 9 - A,B. CD8)Image 249113 (Figure 9 - D,E. Spastin)Figure 6 - E,F,G,H. SpastinImage 249088 (Figure 9 - D,E. CD8)Image 248760 (Figure 1 - A,B)Image 250092 (Figure 9 - A,B. CD8)Image 250324 (Figure 9 - A,B. Spastin)Image 250078 (Figure 9 - A,B. CD8)Image 250143 (Figure 9 - A,B. CD8)Image 250229 (Figure 9 - A,B. EB1-DN)Image 249726 (Figure 3 - G,I. CD8)Image 249851 (Figure 6 - E,F,G,H. EB1-DN)Image 249650 (Figure 3 - I. baz-RNAi)Image 250369 (Figure 9 - A,B. baz-RNAi)Image 249476 (Figure 1 - F,G)Figure 1 - F,GImage 249755 (Figure 3 - G,I. CD8)Image 250364 (Figure 9 - A,B. baz-RNAi)Image 250032 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249900 (Figure 6 - E,F,G,H. Spastin)Image 249730 (Figure 3 - G,I. CD8)Image 249550 (Figure 2 - E,F)Image 249651 (Figure 3 - I. baz-RNAi)Image 249092 (Figure 9 - D,E. CD8)Image 250268 (Supplemental Figure 2 - E,F,G,H)Image 250244 (Figure 9 - A,B. EB1-DN)Image 250129 (Figure 9 - A,B. CD8)Image 248976 (Figure 7 - A,B. RhoGEF2)Image 249597 (Figure 2 - G,H)Image 250342 (Figure 2 - A,B)Image 250245 (Figure 9 - A,B. EB1-DN)Image 249527 (Figure 2 - E,F)Image 248984 (Figure 7 - A,B. RhoGEF2-RNAi)Image 249782 (Figure 4 - A. 1 µm)Image 248979 (Figure 7 - A,B. RhoGEF2-RNAi)Image 248950 (Figure 6 - C,D. Spastin)Image 250237 (Figure 9 - A,B. EB1-DN)Image 250329 (Figure 9 - A,B. Spastin)Image 250150 (Figure 9 - A,B. CD8)Image 250030 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250107 (Figure 9 - A,B. CD8)Image 250236 (Figure 9 - A,B. EB1-DN)Image 250422 (Figure 9 - A,B. en)Image 250378 (Figure 9 - A,B. baz-RNAi)Image 248763 (Figure 1 - A,B)Figure 7 - E,F,G,H. RhoGEF2-RNAiImage 249778 (Figure 4 - A. 1 µm)Image 250133 (Figure 9 - A,B. CD8)Image 249067 (Figure 8 - A,B. Spastin)Image 249614 (Figure 3 - G. baz-RNAi)Image 250139 (Figure 9 - A,B. CD8)Image 250165 (Figure 9 - A,B. CD8)Image 249998 (Figure 7 - E,F,G,H. RhoGEF2)Image 249044 (Figure 8 - A,B. RhoGEF2)Figure 3 - G,I. CD8Image 248917 (Figure 6 - A,B. EB1-DN)Figure 2 - E,FImage 249944 (Figure 6 - E,F,G,H. Spastin)Image 250088 (Figure 9 - A,B. CD8)Image 250423 (Figure 9 - A,B. en)Image 248948 (Figure 6 - C,D. Spastin)Image 250126 (Figure 9 - A,B. CD8)Figure 2 - C,DImage 249630 (Figure 3 - G. baz-RNAi)Image 249615 (Figure 3 - G. baz-RNAi)Image 249165 (Supplemental Figure 2 - A,B. Control)Image 248911 (Figure 5 - C,D,E,F,G,H,I)Image 249170 (Supplemental Figure 2 - C,D. Control)Image 248919 (Figure 6 - A,B. EB1-DN)Image 249942 (Figure 6 - E,F,G,H. Spastin)Image 249631 (Figure 3 - G. baz-RNAi)Image 249523 (Figure 2 - E,F)Image 249794 (Figure 4 - A. 2 µm)Image 249556 (Figure 2 - E,F)Image 248805 (Figure 3 - B)Image 249955 (Figure 7 - E,F,G,H. RhoGEF2)Image 250391 (Figure 9 - A,B. baz-RNAi)Image 249160 (Supplemental Figure 2 - A,B. Control)Image 249004 (Figure 7 - C,D. RhoGEF2-RNAi)Image 249028 (Figure 8 - A,B. CD8)Figure 1 - D,EImage 249940 (Figure 6 - E,F,G,H. Spastin)Image 248863 (Figure 2 - C,D)Image 248897 (Figure 5 - C,D,E,F,G,H,I)Image 249591 (Figure 2 - G,H)Image 249175 (Supplemental Figure 2 - C,D. Control)Image 248828 (Figure 3 - C,E)Image 248842 (Figure 3 - D,F)Image 250305 (Figure 9 - A,B. Spastin)Image 249953 (Figure 7 - E,F,G,H. RhoGEF2)Image 249957 (Figure 7 - E,F,G,H. RhoGEF2)Image 249043 (Figure 8 - A,B. RhoGEF2)Image 248916 (Figure 6 - A,B. EB1-DN)Image 250235 (Figure 9 - A,B. EB1-DN)Image 249098 (Figure 9 - D,E. EB1-DN)Image 250406 (Figure 9 - A,B. en)Image 249609 (Figure 3 - G. baz-RNAi)Image 249068 (Figure 8 - A,B. Spastin)Image 249720 (Figure 3 - G,I. CD8)Image 250050 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Figure 9 - D,E. EB1-DNImage 250162 (Figure 9 - A,B. CD8)Image 249176 (Supplemental Figure 2 - C,D. Control)Image 250001 (Figure 7 - E,F,G,H. RhoGEF2)Image 250290 (Figure 9 - A,B. Spastin)Image 248771 (Figure 1 - D,E)Image 249138 (Supplemental Figure 2 - A,B. eb1)Supplemental Figure 1Supplemental Figure 2Image 248766 (Figure 1 - A,B)Image 249988 (Figure 7 - E,F,G,H. RhoGEF2)Image 249770 (Figure 4 - A. 1 µm)Image 250334 (Figure 2 - A,B)Image 250017 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Supplemental Figure 2 - C,D. ControlImage 249641 (Figure 3 - I. baz-RNAi)Image 249961 (Figure 7 - E,F,G,H. RhoGEF2)Image 249139 (Supplemental Figure 2 - A,B. eb1)Image 250323 (Figure 9 - A,B. Spastin)Image 250052 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250318 (Figure 9 - A,B. Spastin)Image 249743 (Figure 3 - G,I. CD8)Image 249802 (Figure 4 - A. 2 µm)Image 248970 (Figure 7 - A,B. RhoGEF2)Image 249535 (Figure 2 - E,F)Image 249845 (Figure 4 - C,D)Image 250371 (Figure 9 - A,B. baz-RNAi)Image 249684 (Figure 1 - F,G)Figure 2 - A,BImage 249776 (Figure 4 - A. 1 µm)Image 248895 (Figure 5 - C,D,E,F,G,H,I)Image 249032 (Figure 8 - A,B. EB1-DN)Image 250437 (Figure 9 - A,B. en)Image 250095 (Figure 9 - A,B. CD8)Image 250387 (Figure 9 - A,B. baz-RNAi)Image 250243 (Figure 9 - A,B. EB1-DN)Image 250210 (Figure 9 - A,B. EB1-DN)Image 250239 (Figure 9 - A,B. EB1-DN)Image 248767 (Figure 1 - A,B)Image 249040 (Figure 8 - A,B. EB1-DN)Image 249490 (Figure 2 - C,D)Image 250332 (Figure 2 - A,B)Image 250404 (Figure 9 - A,B. en)Image 249164 (Supplemental Figure 2 - A,B. Control)Image 250121 (Figure 9 - A,B. CD8)Image 250419 (Figure 9 - A,B. en)Image 248971 (Figure 7 - A,B. RhoGEF2)Image 249052 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250155 (Figure 9 - A,B. CD8)Image 250435 (Figure 9 - A,B. en)Image 250117 (Figure 9 - A,B. CD8)Image 249716 (Figure 3 - G,I. CD8)Image 249645 (Figure 3 - I. baz-RNAi)Image 249653 (Figure 3 - I. baz-RNAi)Image 249025 (Figure 8 - A,B. CD8)Image 249886 (Figure 6 - E,F,G,H. EB1-DN)Image 248944 (Figure 6 - C,D. Spastin)Image 249095 (Figure 9 - D,E. CD8)Image 248939 (Figure 6 - C,D. EB1-DN)Image 250291 (Figure 9 - A,B. Spastin)Image 248774 (Figure 1 - D,E)Image 250247 (Figure 9 - A,B. EB1-DN)Image 248926 (Figure 6 - A,B. Spastin)Image 248969 (Figure 7 - A,B. RhoGEF2)Image 249104 (Figure 9 - D,E. EB1-DN)Image 250340 (Figure 2 - A,B)Image 250158 (Figure 9 - A,B. CD8)Image 248902 (Figure 5 - C,D,E,F,G,H,I)Image 249189 (Supplemental Figure 2 - C,D. eb1)Supplemental Figure 1 - A,BImage 250421 (Figure 9 - A,B. en)Image 249856 (Figure 6 - E,F,G,H. EB1-DN)Image 249077 (Figure 9 - D,E. baz-RNAi)Image 249677 (Figure 3 - I. baz-RNAi)Image 248844 (Figure 3 - D,F)Image 250003 (Figure 7 - E,F,G,H. RhoGEF2)Image 248928 (Figure 6 - A,B. Spastin)Image 249152 (Supplemental Figure 2 - A,B. Control)Image 250080 (Figure 9 - A,B. CD8)Image 249167 (Supplemental Figure 2 - C,D. Control)Image 248861 (Figure 2 - C,D)Image 250361 (Figure 9 - A,B. baz-RNAi)Figure 3 - AImage 249080 (Figure 9 - D,E. baz-RNAi)Figure 3 - BImage 248837 (Figure 3 - D,F)Image 250203 (Supplemental Figure 1 - C,D,E,F)Image 250056 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250068 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249583 (Figure 2 - G,H)Image 248892 (Figure 5 - A)Image 250114 (Figure 9 - A,B. CD8)Image 250195 (Supplemental Figure 1 - C,D,E,F)Image 250436 (Figure 9 - A,B. en)Image 248927 (Figure 6 - A,B. Spastin)Image 250098 (Figure 9 - A,B. CD8)Image 249496 (Figure 2 - C,D)Image 250373 (Figure 9 - A,B. baz-RNAi)Image 250300 (Figure 9 - A,B. Spastin)Image 248904 (Figure 5 - C,D,E,F,G,H,I)Image 250385 (Figure 9 - A,B. baz-RNAi)Image 250136 (Figure 9 - A,B. CD8)Image 250140 (Figure 9 - A,B. CD8)Image 250289 (Figure 9 - A,B. Spastin)Image 249521 (Figure 2 - E,F)Image 249106 (Figure 9 - D,E. EB1-DN)Image 250036 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250145 (Figure 9 - A,B. CD8)Image 250292 (Figure 9 - A,B. Spastin)Image 249191 (Supplemental Figure 2 - C,D. eb1)Image 250375 (Figure 9 - A,B. baz-RNAi)Image 249030 (Figure 8 - A,B. CD8)Image 249633 (Figure 3 - G. baz-RNAi)Image 249784 (Figure 4 - A. 1 µm)Image 249057 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250062 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250120 (Figure 9 - A,B. CD8)Image 249190 (Supplemental Figure 2 - C,D. eb1)Image 250416 (Figure 9 - A,B. en)Image 249090 (Figure 9 - D,E. CD8)Image 249831 (Figure 4 - C,D)Image 248923 (Figure 6 - A,B. Spastin)Image 250211 (Figure 9 - A,B. EB1-DN)Image 250185 (Supplemental Figure 1 - C,D,E,F)Image 249192 (Supplemental Figure 2 - C,D. eb1)Image 248978 (Figure 7 - A,B. RhoGEF2-RNAi)Image 250288 (Figure 9 - A,B. Spastin)Image 250407 (Figure 9 - A,B. en)Image 249928 (Figure 6 - E,F,G,H. Spastin)Image 250241 (Figure 9 - A,B. EB1-DN)Image 249854 (Figure 6 - E,F,G,H. EB1-DN)Image 248900 (Figure 5 - C,D,E,F,G,H,I)Image 250138 (Figure 9 - A,B. CD8)Image 250309 (Figure 9 - A,B. Spastin)Image 250248 (Figure 9 - A,B. EB1-DN)Image 249103 (Figure 9 - D,E. EB1-DN)Image 249646 (Figure 3 - I. baz-RNAi)Image 249764 (Figure 3 - G,I. CD8)Image 249161 (Supplemental Figure 2 - A,B. Control)Image 249023 (Figure 8 - A,B. CD8)Image 249179 (Supplemental Figure 2 - C,D. Control)Supplemental Figure 2 - A,B. eb1Image 249869 (Figure 6 - E,F,G,H. EB1-DN)Image 248983 (Figure 7 - A,B. RhoGEF2-RNAi)Image 249184 (Supplemental Figure 2 - C,D. eb1)Image 249041 (Figure 8 - A,B. EB1-DN)Image 250173 (Supplemental Figure 1 - C,D,E,F)Image 249530 (Figure 2 - E,F)Image 249933 (Figure 6 - E,F,G,H. Spastin)Image 250350 (Figure 2 - A,B)Image 250425 (Figure 9 - A,B. en)Image 249519 (Figure 2 - E,F)Image 249549 (Figure 2 - E,F)Image 249780 (Figure 4 - A. 1 µm)Image 248996 (Figure 7 - C,D. RhoGEF2)Image 249070 (Figure 8 - A,B. Spastin)Image 250352 (Figure 2 - A,B)Image 248921 (Figure 6 - A,B. EB1-DN)Image 249183 (Supplemental Figure 2 - C,D. eb1)Image 250420 (Figure 9 - A,B. en)Image 249739 (Figure 3 - G,I. CD8)Image 250106 (Figure 9 - A,B. CD8)Image 248915 (Figure 6 - A,B. EB1-DN)Image 249766 (Figure 4 - A. 1 µm)Image 250175 (Supplemental Figure 1 - C,D,E,F)Image 250094 (Figure 9 - A,B. CD8)Image 249048 (Figure 8 - A,B. RhoGEF2)Image 248838 (Figure 3 - D,F)Image 249001 (Figure 7 - C,D. RhoGEF2-RNAi)Image 248914 (Figure 6 - A,B. EB1-DN)Image 249532 (Figure 2 - E,F)Figure 6 - C,D. SpastinImage 250434 (Figure 9 - A,B. en)Image 249806 (Figure 4 - A. 2 µm)Image 249891 (Figure 6 - E,F,G,H. EB1-DN)Image 250388 (Figure 9 - A,B. baz-RNAi)Image 250393 (Figure 9 - A,B. baz-RNAi)Image 250097 (Figure 9 - A,B. CD8)Image 250225 (Figure 9 - A,B. EB1-DN)Image 250306 (Figure 9 - A,B. Spastin)Image 249963 (Figure 7 - E,F,G,H. RhoGEF2)Image 248834 (Figure 3 - C,E)Image 249155 (Supplemental Figure 2 - A,B. Control)Image 249118 (Supplemental Figure 1 - A,B)Image 248932 (Figure 6 - A,B. Spastin)Image 249533 (Figure 2 - E,F)Image 249847 (Figure 4 - C,D)Image 250182 (Supplemental Figure 1 - C,D,E,F)Image 249180 (Supplemental Figure 2 - C,D. Control)Figure 3 - C,EImage 249698 (Figure 3 - G,I. CD8)Image 249026 (Figure 8 - A,B. CD8)Image 248759 (Figure 1 - A,B)Image 250240 (Figure 9 - A,B. EB1-DN)Image 250304 (Figure 9 - A,B. Spastin)Image 250070 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250177 (Supplemental Figure 1 - C,D,E,F)Image 248891 (Figure 5 - A)Image 249575 (Figure 2 - G,H)Image 250234 (Figure 9 - A,B. EB1-DN)Image 248761 (Figure 1 - A,B)Image 249714 (Figure 3 - G,I. CD8)Image 250219 (Figure 9 - A,B. EB1-DN)Figure 8 - A,B. CD8Image 250285 (Figure 9 - A,B. Spastin)Image 249931 (Figure 6 - E,F,G,H. Spastin)Image 248835 (Figure 3 - C,E)Image 250379 (Figure 9 - A,B. baz-RNAi)Image 249150 (Supplemental Figure 2 - A,B. eb1)Image 249494 (Figure 2 - C,D)Image 250218 (Figure 9 - A,B. EB1-DN)Image 250109 (Figure 9 - A,B. CD8)Image 249930 (Figure 6 - E,F,G,H. Spastin)Image 250220 (Figure 9 - A,B. EB1-DN)Image 249947 (Figure 6 - E,F,G,H. EB1-DN)Image 250392 (Figure 9 - A,B. baz-RNAi)Image 249085 (Figure 9 - D,E. baz-RNAi)Image 249005 (Figure 7 - C,D. RhoGEF2-RNAi)Image 250135 (Figure 9 - A,B. CD8)Image 249145 (Supplemental Figure 2 - A,B. eb1)Image 250152 (Figure 9 - A,B. CD8)Image 249488 (Figure 2 - C,D)Image 249627 (Figure 3 - G. baz-RNAi)Image 250060 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249563 (Figure 2 - E,F)Image 249516 (Figure 2 - E,F)Figure 3 - G. baz-RNAiImage 249045 (Figure 8 - A,B. RhoGEF2)Image 248839 (Figure 3 - D,F)Image 249571 (Figure 2 - G,H)Image 250148 (Figure 9 - A,B. CD8)Image 248831 (Figure 3 - C,E)Image 249967 (Figure 7 - E,F,G,H. RhoGEF2)Image 249624 (Figure 3 - G. baz-RNAi)Image 250015 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250295 (Figure 9 - A,B. Spastin)Image 250338 (Figure 2 - A,B)Image 249979 (Figure 7 - E,F,G,H. RhoGEF2)Image 249617 (Figure 3 - G. baz-RNAi)Image 250367 (Figure 9 - A,B. baz-RNAi)Image 249965 (Figure 7 - E,F,G,H. RhoGEF2)Image 249508 (Figure 2 - C,D)Image 249581 (Figure 2 - G,H)Image 249640 (Figure 3 - G. baz-RNAi)Image 250411 (Figure 9 - A,B. en)Image 248947 (Figure 6 - C,D. Spastin)Image 250113 (Figure 9 - A,B. CD8)Image 249117 (Figure 9 - D,E. Spastin)Image 250307 (Figure 9 - A,B. Spastin)Image 249143 (Supplemental Figure 2 - A,B. eb1)Image 249815 (Figure 4 - C,D)Image 250224 (Figure 9 - A,B. EB1-DN)Image 249745 (Figure 3 - G,I. CD8)Image 250380 (Figure 9 - A,B. baz-RNAi)Figure 4 - A. 2 µmImage 249159 (Supplemental Figure 2 - A,B. Control)Image 250250 (Figure 9 - A,B. EB1-DN)Image 249566 (Figure 2 - E,F)Image 248827 (Figure 3 - C,E)Image 250360 (Figure 9 - A,B. baz-RNAi)Image 249736 (Figure 3 - G,I. CD8)Image 248775 (Figure 1 - D,E)Image 249492 (Figure 2 - C,D)Image 249089 (Figure 9 - D,E. CD8)Image 250396 (Figure 9 - A,B. baz-RNAi)Image 249111 (Figure 9 - D,E. Spastin)Image 248826 (Figure 3 - C,E)Image 249564 (Figure 2 - E,F)Image 249656 (Figure 3 - I. baz-RNAi)Image 249811 (Figure 4 - A. 2 µm)Image 248986 (Figure 7 - A,B. RhoGEF2-RNAi)Image 248990 (Figure 7 - C,D. RhoGEF2)Image 250119 (Figure 9 - A,B. CD8)Image 250021 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 250130 (Figure 9 - A,B. CD8)Figure 2 - G,HImage 248832 (Figure 3 - C,E)Image 249093 (Figure 9 - D,E. CD8)Figure 7 - C,D. RhoGEF2Image 250297 (Figure 9 - A,B. Spastin)Image 250402 (Figure 9 - A,B. en)Image 249047 (Figure 8 - A,B. RhoGEF2)Image 250439 (Figure 9 - A,B. en)Image 249926 (Figure 6 - E,F,G,H. Spastin)Image 248997 (Figure 7 - C,D. RhoGEF2)Image 249971 (Figure 7 - E,F,G,H. RhoGEF2)Image 250038 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249813 (Figure 4 - A. 2 µm)Image 249817 (Figure 4 - C,D)Image 250441 (Figure 9 - A,B. en)Image 248949 (Figure 6 - C,D. Spastin)Image 249620 (Figure 3 - G. baz-RNAi)Image 250399 (Figure 9 - A,B. en)Image 250400 (Figure 9 - A,B. en)Image 250298 (Figure 9 - A,B. Spastin)Image 250359 (Figure 9 - A,B. baz-RNAi)Image 250228 (Figure 9 - A,B. EB1-DN)Image 250180 (Supplemental Figure 1 - C,D,E,F)Image 249115 (Figure 9 - D,E. Spastin)Image 249871 (Figure 6 - E,F,G,H. EB1-DN)Figure 9 - A,B. SpastinImage 249151 (Supplemental Figure 2 - A,B. eb1)Image 250022 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249137 (Supplemental Figure 2 - A,B. eb1)Image 249704 (Figure 3 - G,I. CD8)Image 249924 (Figure 6 - E,F,G,H. Spastin)Image 248890 (Figure 5 - A)Image 248776 (Figure 1 - D,E)Image 250238 (Figure 9 - A,B. EB1-DN)Image 248991 (Figure 7 - C,D. RhoGEF2)Image 249114 (Figure 9 - D,E. Spastin)Image 248770 (Figure 1 - D,E)Image 249158 (Supplemental Figure 2 - A,B. Control)Image 250376 (Figure 9 - A,B. baz-RNAi)Image 248907 (Figure 5 - C,D,E,F,G,H,I)Figure 8 - A,B. EB1-DNImage 250082 (Figure 9 - A,B. CD8)Image 249086 (Figure 9 - D,E. CD8)Image 249558 (Figure 2 - E,F)Image 249000 (Figure 7 - C,D. RhoGEF2-RNAi)Image 249035 (Figure 8 - A,B. EB1-DN)Image 250261 (Supplemental Figure 2 - E,F,G,H)Image 248993 (Figure 7 - C,D. RhoGEF2)Image 250308 (Figure 9 - A,B. Spastin)Image 250054 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249112 (Figure 9 - D,E. Spastin)Figure 9 - A,B. enImage 249573 (Figure 2 - G,H)Figure 9 - D,E. baz-RNAiImage 249081 (Figure 9 - D,E. baz-RNAi)Image 249599 (Figure 2 - G,H)Image 249864 (Figure 6 - E,F,G,H. EB1-DN)Image 250116 (Figure 9 - A,B. CD8)Image 249076 (Figure 9 - D,E. baz-RNAi)Image 250283 (Supplemental Figure 2 - E,F,G,H)Image 249804 (Figure 4 - A. 2 µm)Image 249049 (Figure 8 - A,B. RhoGEF2)Image 250142 (Figure 9 - A,B. CD8)Image 249823 (Figure 4 - C,D)Image 249053 (Figure 8 - A,B. RhoGEF2-RNAi)Image 250123 (Figure 9 - A,B. CD8)Image 248995 (Figure 7 - C,D. RhoGEF2)Image 250432 (Figure 9 - A,B. en)Image 249110 (Figure 9 - D,E. Spastin)Figure 8 - A,B. RhoGEF2-RNAiImage 249796 (Figure 4 - A. 2 µm)Image 250372 (Figure 9 - A,B. baz-RNAi)Image 249182 (Supplemental Figure 2 - C,D. eb1)Image 248903 (Figure 5 - C,D,E,F,G,H,I)Image 250336 (Figure 2 - A,B)Image 249107 (Figure 9 - D,E. EB1-DN)Image 250164 (Figure 9 - A,B. CD8)Image 250101 (Figure 9 - A,B. CD8)Image 249603 (Figure 2 - G,H)Image 250384 (Figure 9 - A,B. baz-RNAi)Image 249039 (Figure 8 - A,B. EB1-DN)Image 248768 (Figure 1 - A,B)Image 249157 (Supplemental Figure 2 - A,B. Control)Image 249672 (Figure 3 - I. baz-RNAi)Image 249929 (Figure 6 - E,F,G,H. Spastin)Image 249666 (Figure 3 - I. baz-RNAi)Image 248762 (Figure 1 - A,B)Image 248936 (Figure 6 - C,D. EB1-DN)Image 248833 (Figure 3 - C,E)Image 249486 (Figure 1 - F,G)Image 249099 (Figure 9 - D,E. EB1-DN)Image 249144 (Supplemental Figure 2 - A,B. eb1)Image 250046 (Figure 7 - E,F,G,H. RhoGEF2-RNAi)Image 249480 (Figure 1 - F,G)Image 248994 (Figure 7 - C,D. RhoGEF2)Image 249518 (Figure 2 - E,F)Figure 9 - D,E. SpastinAnna AkhmanovaNicholas H. BrownAlpha S. YapNatalia A. BulgakovaIlya GrigorievfalseDynamic microtubules produce an asymmetric E-cadherin–Bazooka complex to maintain segment boundaries2013-06-10T11:24:10Z2018-11-29T11:24:10Z2018-11-29T11:24:10ZS-JCBD-20121115910.1083/jcb.201211159