bioimagesUnknownChelsi J. SnowThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201212117JCB

Immunofluorescence microscopy for Ran (red), GFP-STV-SV40NLS (green), and Tpr (purple) during mitosis.

Tip60 (green) and Ran (red) in siControl siRNA and siNTF2 cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy showing the localization of Tpr (red) and its NPC docking partner Nup153 (green) in normal (8469) primary fibroblasts.

Tip60 (green) and Ran (red) in siControl siRNA cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy of cells expressing TprNLS delta 1812-1828 stained for nuclei (DAPI, blue) and Myc (green).

GFP-STV-SV40NLS (green) and Ran (red) in siControl siRNA-treated cells. Nuclei are marked with DAPI (blue).

Myc-PK-SV40NLS (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

P400 (green) in HA-Progerin (red)-transfected cells. Nuclei are marked with DAPI (blue).

Myc-PK-TprNLS (green) and Ran (red) in siControl siRNA cells. Nuclei are marked with DAPI (blue).

GFP-STV-SV40NLS (green) co-transfected with HA-Progerin (red).

Orc2 (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy showing the localization of Tpr (red) and its NPC docking partner Nup153 (green) in HGPS (1498) primary fibroblasts.

Immunofluorescence microscopy for Nup153 (red), GFP-STV-SV40NLS (green) and Tpr (purple) during mitosis.

Tip60 (green) in HA-Progerin (red)-transfected cells. Nuclei are marked with DAPI (blue).

P400 (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy of Tpr (red) and Ran (green) in HGPS 1972 fibroblasts. Nuclei are stained by DAPI (blue).

Immunofluorescence microscopy showing the localization of Tpr (red) and its NPC docking partner Nup153 (green) HGPS (1972) primary fibroblasts.

GFP-STV-SV40NLS (green) co-transfected with HA-Progerin C611S (red).

Tpr (green) in siControl cells. Nuclei are marked by DAPI (blue).

Immunofluorescence microscopy of cells expressing TprNLS K1857A K1858A stained for nuclei (DAPI, blue) and Myc (green).

Immunofluorescence microscopy of Tpr (green) in HeLa cells treated with siKPNA2. Nuclei stained with DAPI, blue.

Myc-Tip60 (green) in HA-Progerin C611S (red)-transfected cells. Nuclei are marked with DAPI (blue).

Tpr (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

Triple-label immunofluorescence microscopy of HeLa cells transfected with HA-Progerin with a CAAX motif substitution (C611S; red). Endogenous Tpr (purple) and GFP-STV-SV40NLS (green) were also detected.

Immunofluorescence microscopy of cells expressing TprNLS K1829A R1830A stained for nuclei (DAPI, blue) and Myc (green).

Tpr (red) and Ran (green) in control-depleted HeLa cells. Nuclei are stained by DAPI (blue)

Immunofluorescence microscopy of cells expressing TprNLS delta 1861-1867 stained for nuclei (DAPI, blue) and Myc (green).

Tpr (green) and Ran (red) in siControl siRNA cells. Nuclei are marked with DAPI (blue).

Myc-Tpr (green), in which the endogenous TprNLS was replaced with SV40NLS, co-transfected with HA-Progerin (red).

Triple-label immunofluorescence microscopy of HeLa cells transfected with HA-Progerin (red). Endogenous Tpr (purple) and GFP-STV-SV40NLS (green) were also detected.

P400 (green) and Ran (red) in siControl siRNAcells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy showing the localization of Tpr (red) and its NPC docking partner Nup153 (green) in HGPS (3199) primary fibroblasts.

Myc-PK-TprNLS (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy of Tpr (green) in HeLa cells treated with control siRNA. Nuclei stained with DAPI, blue.

Myc-Tpr (green), in which the endogenous TprNLS was replaced with SV40NLS, co-transfected with HA-Progerin C611S (red).

GFP-STV-SV40NLS (green) and Ran (red) in siNTF2 cells. Nuclei are marked with DAPI (blue).

Myc-PK-SV40NLS (green) co-transfected with HA-Progerin (red).

Immunofluorescence microscopy of TprNLS wild-type cells stained for nuclei (DAPI, blue) and Myc (green).

Myc-Tip60 (green) in HA-Progerin (red)-transfected cells. Nuclei are marked with DAPI (blue).

Myc-PK-SV40NLS (green) co-transfected with HA-Progerin C611S.

Tip60 (green) in HA-Progerin C611S (red)-transfected cells. Nuclei are marked with DAPI (blue).

Orc2 (green) and Ran (red) in siControl siRNA cells. Nuclei are marked with DAPI (blue).

Orc2 (green) in HA-Progerin C611S (red)-transfected cells. Nuclei are marked with DAPI (blue).

P400 (green) in HA-Progerin C611S (red)-transfected cells. Nuclei are marked with DAPI (blue).

Depletion of Tpr by siRNA reduces antibody signal by IF microscopy (green), indicating it is specific for Tpr. Nuclei are marked by DAPI (blue).

Myc-PK-SV40NLS (green) and Ran (red) in siControl siRNA cells. Nuclei are marked with DAPI (blue).

Orc2 (green) in HA-Progerin (red)-transfected cells. Nuclei are marked with DAPI (blue).

Immunofluorescence microscopy of Tpr (green) in HeLa cells treated with siKPNA1. Nuclei stained with DAPI, blue.

Immunofluorescence microscopy of Tpr (red) and Ran (green) in normal 8469 fibroblasts. Nuclei are stained by DAPI (blue).

Myc-PK-TprNLS (green) co-transfected with HA-Progerin (red).

Tpr (red) and Ran (green) in NTF2-depleted HeLa cells. Nuclei are stained by DAPI (blue).

Myc-PK-TprNLS (green) co-transfected with HA-Progerin C611S.

bioimagesImage 147154 (Figure 1 - A)Figure 10 - EFigure 8 - EFigure 10 - GFigure 8 - CSupplemental Figure 5Figure 10 - AFigure 8 - ASupplemental Figure 2Figure 10 - CImage 147132 (Supplemental Figure 2 - C)Image 147092 (Figure 4 - A)Image 147129 (Figure 10 - G)Image 147114 (Figure 9 - A)Figure 9 - GImage 147094 (Figure 4 - A)Image 147102 (Figure 5 - B)Supplemental Figure 2 - EFigure 9 - CFigure 9 - ESupplemental Figure 2 - ASupplemental Figure 2 - CFigure 9 - AImage 147085 (Figure 3 - A)Image 147131 (Supplemental Figure 2 - A)Image 147097 (Figure 4 - B)Image 147109 (Figure 8 - C)Figure 8 - GImage 147119 (Figure 9 - E)Image 147100 (Figure 5 - B)Image 147079 (Figure 1 - A)Image 147111 (Figure 8 - E)Figure 1 - AImage 147090 (Figure 4 - A)Image 147134 (Supplemental Figure 2 - E)Image 147083 (Figure 2 - A)Image 147118 (Figure 9 - E)Image 147082 (Figure 2 - A)Image 147127 (Figure 10 - E)Image 147133 (Supplemental Figure 2 - C)Image 147107 (Figure 8 - A)Image 147137 (Supplemental Figure 5 - A)Figure 2 - AImage 147101 (Figure 5 - B)Image 147123 (Figure 10 - A)Image 147126 (Figure 10 - E)Image 147080 (Figure 1 - A)Image 147096 (Figure 4 - B)Image 147136 (Supplemental Figure 5 - A)Figure 3 - AImage 147084 (Figure 3 - A)Image 147116 (Figure 9 - C)Image 147106 (Figure 8 - A)Figure 3 - CFigure 4 - AImage 147103 (Figure 6 - C)Figure 4 - BImage 147110 (Figure 8 - E)Image 147098 (Figure 5 - B)Image 147125 (Figure 10 - C)Image 147128 (Figure 10 - G)Image 147091 (Figure 4 - A)Image 147135 (Supplemental Figure 2 - E)Image 147156 (Figure 8 - G)Image 147115 (Figure 9 - A)Image 147104 (Figure 6 - C)Image 147099 (Figure 5 - B)Image 147122 (Figure 10 - A)Image 147130 (Supplemental Figure 2 - A)Figure 5 - BImage 147086 (Figure 3 - C)Image 147108 (Figure 8 - C)Image 147155 (Figure 8 - G)Image 147120 (Figure 9 - G)Figure 5Figure 4Image 147087 (Figure 3 - C)Figure 6Figure 10Figure 9Image 147088 (Figure 4 - A)Figure 8Image 147105 (Figure 6 - C)Image 147081 (Figure 1 - A)Figure 6 - CImage 147089 (Figure 4 - A)Figure 1Supplemental Figure 5 - AImage 147124 (Figure 10 - C)Figure 3Image 147095 (Figure 4 - B)Image 147117 (Figure 9 - C)Figure 2Image 147093 (Figure 4 - A)Image 147121 (Figure 9 - G)Ashraf DarAnindya DuttaChelsi J. SnowBryce M. PaschalRalph H. KehlenbachfalseDefective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport2013-05-06T11:24:33Z2018-11-29T11:24:33Z2018-11-29T11:24:33ZS-JCBD-20121211710.1083/jcb.201212117