{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Felix Bärenz"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201302122"],"attach_to":["JCB"],"legend":["Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP; blue: chromatin. <br />","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for microtubules (alpha-tubulin).","GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos.  Time, 2 min.","Indirect immunofluorescence for gamma-Tubulin of SSX2IP knockdown 1 human RPE-1 cells in mitosis.","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: XGrip210/GCP6.<br /><br />","Chromatin beads spindles were assembled in Xenopus egg extracts with Dynein antibody added. Red: microtubules; green: SSX2IP; blue: chromatin. <br />","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP depleted in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.","Indirect immunofluorescence of a human RPE-1 cell in mitosis. Green: microtubules; red: SSX2IP; blue: DNA.","Chromatin beads spindles were assembled in Xenopus egg extracts with control IgG was added. Red: microtubules; green: SSX2IP; blue: chromatin. <br />","Indirect immunofluorescence of SSX2IP in a human RPE-1 cell in mitosis.","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for gamma-tubulin.","Indirect immunofluorescence detects gamma-tubulin (green) and DNA (blue) in stage 10 Medaka blastomeres.","DAPI staining of SSX2IP knockdown 2 human RPE-1 cells in mitosis.","Sperm nuclei spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: SSX2IP; blue: chromatin.","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: XGrip109/GCP3.","PCM-1 (green) and SSX2IP (red) were detected in human RPE-1 cells in interphase by interdirect immunofluorescence.","DAPI staining of a human RPE-1 cell in interphase.","Indirect immunofluorescence for alpha-Tubulin in SSX2IP knockdown 1 human RPE-1 cells in mitosis.","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin.  Inset from  gamma-tubulin-stained image.","GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 6 min.","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: XGrip109/GCP3.","Indirect immunofluorescence for alpha-Tubulin in a human RPE-1 cell in interphase.","GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 0 min.","Indirect immunofluorescence detects gamma-tubulin (red) and DNA (blue) in control blastomeres.","Chromatin beads spindles were assembled in Xenopus egg extracts depleted of TPX2. Red: microtubules; green: SSX2IP; blue: chromatin.","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP in the presence of RanGTP. Red: microtubules; green: SSX2IP.","Indirect immunofluorescence detects SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.","Chromatin bead spindles were assembled in Xenopus egg extracts with anti-SSX2IP IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: gamma-tubulin.","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for chromatin (DAPI).","Chromatin beads spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: SSX2IP; blue: chromatin.","Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.","Indirect immunofluorescence for gamma-Tubulin in control human RPE-1 cells in mitosis.","Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: XGrip210/GCP6.<br /><br />","GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 2 min.","Indirect immunofluorescence of SSX2IP in a human RPE-1 cell in interphase.","Indirect immunofluorescence of a human RPE-1 cell in interphase. Green: microtubules; red: SSX2IP; blue: DNA.","DAPI staining of control human RPE-1 cells in mitosis.","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: gamma-tubulin.","Chromatin bead spindles were assembled in Xenopus egg extracts with control IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin. Red: microtubules; green: gamma-tubulin; blue: chromatin.","Indirect immunofluorescence of SSX2IP knockdown 2 human RPE-1 cells in mitosis. Green: microtubules; red: SSX2IP; blue: DAPI.","Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for microtubules (alpha-tubulin).","Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for gamma-tubulin.","Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for chromatin (DAPI).","Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: SSX2IP.","Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of TPX2. Red: microtubules; green: SSX2IP; blue: chromatin. <br />","gamma-tubulin (blue) and SSX2IP (red) were detected in human RPE-1 cells in metaphase by interdirect immunofluorescence.","Sperm nuclei spindles were assembled in control Xenopus egg extracts. Inset from gamma-tubulin-stained image.","Indirect immunofluorescence detects alpha-tubulin (green) and DNA (blue) in stage 10 Medaka blastomeres.","GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos.  Time, 4 min.","Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: gamma-tubulin.","Indirect immunofluorescence of human RPE-1 cells in mitosis (control). Green: microtubules; red: SSX2IP; blue: DAPI.","Sperm nuclei spindles were assembled in Xenopus egg extracts with Dynein IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.<br /><br />","Indirect immunofluorescence detects PCM-1 (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.","Indirect immunofluorescence detects gamma-tubulin (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.","PCM-1 (green) and SSX2IP (red) were detected in human RPE-1 cells in metaphase by interdirect immunofluorescence.","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.","Indirect immunofluorescence detects gamma-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.<br />","Sperm nuclei spindles were assembled in Xenopus egg extracts with control IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.","Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in control blastomeres.","Indirect immunofluorescence detects gamma-tubulin (red), SSX2IP (GFP; green) and DNA (blue) in blastomeres rescued for SSX2IP.","Chromatin bead spindles were assembled in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP; blue: chromatin.","Sperm nuclei spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: gamma-tubulin; blue: chromatin.","Sperm nuclei spindles were assembled in Xenopus egg extracts, rescue. Red: microtubules; green: SSX2IP; blue: chromatin.","gamma-tubulin (blue) and SSX2IP (red) were detected in human RPE-1 cells in interphase by interdirect immunofluorescence.","Staining by indirect immunofluorescence detects microtubules (red) and SSX2IP (green) in oocytes that were injected with morpholinos against SSX2IP.","GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 4 min.","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP.","GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos.  Time, 6 min.","Indirect immunofluorescence detects alpha-tubulin (red), SSX2IP (GFP; green) and DNA (blue) in blastomeres rescued for SSX2IP.","Indirect immunofluorescence for gamma-tubulin of SSX2IP knockdown 2 human RPE-1 cells in mitosis.","Centrosome nucleation was tested in control Xenopus egg extracts in the presence of RanGTP. Red: microtubules; green: SSX2IP.","Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.<br />","DAPI staining of SSX2IP knockdown 1 human RPE-1 cells in mitosis.","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: SSX2IP.","Staining by indirect immunofluorescence detects microtubules (red) and SSX2IP (green) in control oocytes.","GFP-Histone 2B was detected in developing control morpholino-injected Medaka embryos. Overview.","Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP in the presence of RanGTP. Red: microtubules; green: SSX2IP.","GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos.  Time, 0 min.","Centrosome nucleation was tested in control Xenopus egg extracts in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.<br /><br />","Indirect immunofluorescence of SSX2IP knockdown 1 human RPE-1 cells in mitosis. Green: microtubules; red: SSX2IP; blue: DAPI.<br /><br />","Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: XGrip210/GCP6.","Indirect immunofluorescence detects alpha-tubulin (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.","Indirect immunofluorescence detects PCM-1 (green) and DNA (blue) in stage 10 Medaka blastomeres.","GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos.  Overview.","DAPI staining of a human RPE-1 cell in mitosis.","Indirect immunofluorescence for alpha-tubulin of SSX2IP knockdown 2 human RPE-1 cells in mitosis.","Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: XGrip109/GCP3.","Indirect immunofluorescence of alpha-tubulin in a human RPE-1 cell in mitosis.","Indirect immunofluorescence for alpha-Tubulin in control human RPE-1 cells in mitosis."],"repository":["bioimages"],"figure_sub":["Image 290126 (Figure 6 - D)","Image 290115 (Figure 6 - A)","Image 290258 (Figure 4 - A)","Image 290271 (Figure 4 - E)","Image 290049 (Figure 3 - A)","Image 290274 (Figure 4 - E)","Image 290129 (Figure 6 - D)","Image 290097 (Figure 5 - B)","Image 290118 (Figure 6 - A)","Image 290323 (Figure 6 - B)","Image 290091 (Figure 5 - A)","Image 290266 (Figure 4 - C)","Image 291496 (Figure 6 - D)","Image 290123 (Figure 6 - D)","Image 290073 (Figure 4 - H)","Image 290269 (Figure 4 - E)","Image 290326 (Figure 6 - B)","Image 290263 (Figure 4 - C)","Image 290122 (Figure 6 - D)","Image 290039 (Figure 2 - B)","Image 290050 (Figure 3 - D)","Image 290037 (Figure 2 - A)","Image 290085 (Figure 5 - A)","Image 290112 (Figure 6 - A)","Image 290260 (Figure 4 - A)","Image 290104 (Figure 5 - F)","Figure 3 - D","Image 290108 (Figure 5 - F)","Image 290103 (Figure 5 - F)","Image 290324 (Figure 6 - B)","Image 290086 (Figure 5 - A)","Image 290098 (Figure 5 - B)","Figure 3 - A","Image 290090 (Figure 5 - A)","Image 290110 (Figure 5 - F)","Image 290119 (Figure 6 - A)","Image 290125 (Figure 6 - D)","Image 290083 (Figure 5 - A)","Image 290074 (Figure 4 - H)","Image 290106 (Figure 5 - F)","Image 290044 (Figure 2 - D)","Figure 5 - A","Figure 5 - F","Image 290080 (Figure 4 - H)","Image 290117 (Figure 6 - A)","Figure 5 - B","Image 290094 (Figure 5 - B)","Figure 5","Figure 4","Image 290268 (Figure 4 - C)","Image 290076 (Figure 4 - H)","Image 290088 (Figure 5 - A)","Image 290096 (Figure 5 - B)","Figure 6","Image 290127 (Figure 6 - D)","Image 290100 (Figure 5 - B)","Figure 3","Figure 2","Image 290075 (Figure 4 - H)","Image 290087 (Figure 5 - A)","Image 290111 (Figure 5 - F)","Image 290038 (Figure 2 - B)","Image 290092 (Figure 5 - B)","Image 290318 (Figure 2 - B)","Image 290109 (Figure 5 - F)","Image 250557 (Figure 2 - A)","Image 290130 (Figure 6 - D)","Image 290133 (Figure 6 - D)","Image 290102 (Figure 5 - F)","Image 290078 (Figure 4 - H)","Image 290052 (Figure 3 - D)","Image 290262 (Figure 4 - A)","Image 290273 (Figure 4 - E)","Image 290320 (Figure 2 - B)","Image 290124 (Figure 6 - D)","Image 290105 (Figure 5 - F)","Image 290265 (Figure 4 - C)","Image 290113 (Figure 6 - A)","Image 290099 (Figure 5 - B)","Image 290272 (Figure 4 - E)","Image 290264 (Figure 4 - C)","Figure 2 - D","Figure 2 - A","Image 290072 (Figure 4 - H)","Figure 2 - B","Image 290270 (Figure 4 - E)","Image 290051 (Figure 3 - D)","Image 290259 (Figure 4 - A)","Image 290261 (Figure 4 - A)","Image 290267 (Figure 4 - C)","Figure 4 - A","Image 290489 (Figure 2 - B)","Image 290079 (Figure 4 - H)","Image 290257 (Figure 4 - A)","Image 290045 (Figure 3 - A)","Figure 4 - H","Figure 4 - E","Figure 4 - C","Image 290093 (Figure 5 - B)","Image 290114 (Figure 6 - A)","Image 290095 (Figure 5 - B)","Image 290089 (Figure 5 - A)","Image 290077 (Figure 4 - H)","Image 290081 (Figure 4 - H)","Image 290321 (Figure 6 - B)","Image 290101 (Figure 5 - B)","Image 290128 (Figure 6 - D)","Image 290132 (Figure 6 - D)","Image 290043 (Figure 2 - D)","Image 290053 (Figure 3 - D)","Image 290256 (Figure 5 - A)","Figure 6 - D","Figure 6 - A","Figure 6 - B","Image 290116 (Figure 6 - A)"],"pubmed_authors":["Joachim Wittbrodt","Maren Klinger","Holger Lorenz","Oliver J. Gruss","Ivana Cado","Kerstin Hupfeld","Daigo Inoue","Hideki Yokoyama","Thomas Ruppert","Herbert Steinbeisser","Dmytro Mayilo","Stephanie Freiss","Burkhard Hoeckendorf","Stefanie Draeger","Sahra Pilz","Felix Bärenz","Sabine Merker","Justus Tegha-Dunghu"],"additional_accession":[]},"is_claimable":false,"name":"The centriolar satellite protein SSX2IP promotes centrosome maturation","description":null,"dates":{"release":"2013-07-01T11:24:52Z","modification":"2018-11-29T11:24:52Z","creation":"2018-11-29T11:24:52Z"},"accession":"S-JCBD-201302122","cross_references":{"doi":["10.1083/jcb.201302122"]}}