bioimagesUnknownFelix BärenzThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201302122JCB

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP; blue: chromatin. <br />

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for microtubules (alpha-tubulin).

GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos. Time, 2 min.

Indirect immunofluorescence for gamma-Tubulin of SSX2IP knockdown 1 human RPE-1 cells in mitosis.

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: XGrip210/GCP6.<br /><br />

Chromatin beads spindles were assembled in Xenopus egg extracts with Dynein antibody added. Red: microtubules; green: SSX2IP; blue: chromatin. <br />

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP depleted in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.

Indirect immunofluorescence of a human RPE-1 cell in mitosis. Green: microtubules; red: SSX2IP; blue: DNA.

Chromatin beads spindles were assembled in Xenopus egg extracts with control IgG was added. Red: microtubules; green: SSX2IP; blue: chromatin. <br />

Indirect immunofluorescence of SSX2IP in a human RPE-1 cell in mitosis.

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for gamma-tubulin.

Indirect immunofluorescence detects gamma-tubulin (green) and DNA (blue) in stage 10 Medaka blastomeres.

DAPI staining of SSX2IP knockdown 2 human RPE-1 cells in mitosis.

Sperm nuclei spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: SSX2IP; blue: chromatin.

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: XGrip109/GCP3.

PCM-1 (green) and SSX2IP (red) were detected in human RPE-1 cells in interphase by interdirect immunofluorescence.

DAPI staining of a human RPE-1 cell in interphase.

Indirect immunofluorescence for alpha-Tubulin in SSX2IP knockdown 1 human RPE-1 cells in mitosis.

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin. Inset from gamma-tubulin-stained image.

GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 6 min.

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: XGrip109/GCP3.

Indirect immunofluorescence for alpha-Tubulin in a human RPE-1 cell in interphase.

GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 0 min.

Indirect immunofluorescence detects gamma-tubulin (red) and DNA (blue) in control blastomeres.

Chromatin beads spindles were assembled in Xenopus egg extracts depleted of TPX2. Red: microtubules; green: SSX2IP; blue: chromatin.

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP in the presence of RanGTP. Red: microtubules; green: SSX2IP.

Indirect immunofluorescence detects SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.

Chromatin bead spindles were assembled in Xenopus egg extracts with anti-SSX2IP IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: gamma-tubulin.

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin and stained for chromatin (DAPI).

Chromatin beads spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: SSX2IP; blue: chromatin.

Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.

Indirect immunofluorescence for gamma-Tubulin in control human RPE-1 cells in mitosis.

Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: XGrip210/GCP6.<br /><br />

GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 2 min.

Indirect immunofluorescence of SSX2IP in a human RPE-1 cell in interphase.

Indirect immunofluorescence of a human RPE-1 cell in interphase. Green: microtubules; red: SSX2IP; blue: DNA.

DAPI staining of control human RPE-1 cells in mitosis.

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: gamma-tubulin.

Chromatin bead spindles were assembled in Xenopus egg extracts with control IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of gamma-tubulin. Red: microtubules; green: gamma-tubulin; blue: chromatin.

Indirect immunofluorescence of SSX2IP knockdown 2 human RPE-1 cells in mitosis. Green: microtubules; red: SSX2IP; blue: DAPI.

Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for microtubules (alpha-tubulin).

Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for gamma-tubulin.

Sperm nuclei spindles were assembled in control Xenopus egg extracts stained for chromatin (DAPI).

Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: SSX2IP.

Sperm nuclei spindles were assembled in Xenopus egg extracts depleted of TPX2. Red: microtubules; green: SSX2IP; blue: chromatin. <br />

gamma-tubulin (blue) and SSX2IP (red) were detected in human RPE-1 cells in metaphase by interdirect immunofluorescence.

Sperm nuclei spindles were assembled in control Xenopus egg extracts. Inset from gamma-tubulin-stained image.

Indirect immunofluorescence detects alpha-tubulin (green) and DNA (blue) in stage 10 Medaka blastomeres.

GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos. Time, 4 min.

Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: gamma-tubulin.

Indirect immunofluorescence of human RPE-1 cells in mitosis (control). Green: microtubules; red: SSX2IP; blue: DAPI.

Sperm nuclei spindles were assembled in Xenopus egg extracts with Dynein IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.<br /><br />

Indirect immunofluorescence detects PCM-1 (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.

Indirect immunofluorescence detects gamma-tubulin (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.

PCM-1 (green) and SSX2IP (red) were detected in human RPE-1 cells in metaphase by interdirect immunofluorescence.

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.

Indirect immunofluorescence detects gamma-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.<br />

Sperm nuclei spindles were assembled in Xenopus egg extracts with control IgG added. Red: microtubules; green: SSX2IP; blue: chromatin.

Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in control blastomeres.

Indirect immunofluorescence detects gamma-tubulin (red), SSX2IP (GFP; green) and DNA (blue) in blastomeres rescued for SSX2IP.

Chromatin bead spindles were assembled in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP; blue: chromatin.

Sperm nuclei spindles were assembled in control Xenopus egg extracts. Red: microtubules; green: gamma-tubulin; blue: chromatin.

Sperm nuclei spindles were assembled in Xenopus egg extracts, rescue. Red: microtubules; green: SSX2IP; blue: chromatin.

gamma-tubulin (blue) and SSX2IP (red) were detected in human RPE-1 cells in interphase by interdirect immunofluorescence.

Staining by indirect immunofluorescence detects microtubules (red) and SSX2IP (green) in oocytes that were injected with morpholinos against SSX2IP.

GFP-Histone 2B was detected in control morpholino-injected developing Medaka embryos. Time, 4 min.

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP. Red: microtubules; green: SSX2IP.

GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos. Time, 6 min.

Indirect immunofluorescence detects alpha-tubulin (red), SSX2IP (GFP; green) and DNA (blue) in blastomeres rescued for SSX2IP.

Indirect immunofluorescence for gamma-tubulin of SSX2IP knockdown 2 human RPE-1 cells in mitosis.

Centrosome nucleation was tested in control Xenopus egg extracts in the presence of RanGTP. Red: microtubules; green: SSX2IP.

Indirect immunofluorescence detects alpha-tubulin (red) and DNA (blue) in SSX2IP morpholino-injected blastomeres.<br />

DAPI staining of SSX2IP knockdown 1 human RPE-1 cells in mitosis.

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: SSX2IP.

Staining by indirect immunofluorescence detects microtubules (red) and SSX2IP (green) in control oocytes.

GFP-Histone 2B was detected in developing control morpholino-injected Medaka embryos. Overview.

Centrosome nucleation was tested in Xenopus egg extracts depleted of SSX2IP in the presence of RanGTP. Red: microtubules; green: SSX2IP.

GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos. Time, 0 min.

Centrosome nucleation was tested in control Xenopus egg extracts in the presence of RanGTP. Red: microtubules; green: gamma-tubulin.<br /><br />

Indirect immunofluorescence of SSX2IP knockdown 1 human RPE-1 cells in mitosis. Green: microtubules; red: SSX2IP; blue: DAPI.<br /><br />

Centrosome nucleation was tested in Xenopus egg extracts rescued for SSX2IP. Red: microtubules; green: XGrip210/GCP6.

Indirect immunofluorescence detects alpha-tubulin (green), SSX2IP (red) and DNA (blue) in stage 10 Medaka blastomeres.

Indirect immunofluorescence detects PCM-1 (green) and DNA (blue) in stage 10 Medaka blastomeres.

GFP-Histone 2B was detected in SSX2IP morpholino-injected developing Medaka embryos. Overview.

DAPI staining of a human RPE-1 cell in mitosis.

Indirect immunofluorescence for alpha-tubulin of SSX2IP knockdown 2 human RPE-1 cells in mitosis.

Centrosome nucleation was tested in control Xenopus egg extracts. Red: microtubules; green: XGrip109/GCP3.

Indirect immunofluorescence of alpha-tubulin in a human RPE-1 cell in mitosis.

Indirect immunofluorescence for alpha-Tubulin in control human RPE-1 cells in mitosis.

bioimagesImage 290126 (Figure 6 - D)Image 290115 (Figure 6 - A)Image 290258 (Figure 4 - A)Image 290271 (Figure 4 - E)Image 290049 (Figure 3 - A)Image 290274 (Figure 4 - E)Image 290129 (Figure 6 - D)Image 290097 (Figure 5 - B)Image 290118 (Figure 6 - A)Image 290323 (Figure 6 - B)Image 290091 (Figure 5 - A)Image 290266 (Figure 4 - C)Image 291496 (Figure 6 - D)Image 290123 (Figure 6 - D)Image 290073 (Figure 4 - H)Image 290269 (Figure 4 - E)Image 290326 (Figure 6 - B)Image 290263 (Figure 4 - C)Image 290122 (Figure 6 - D)Image 290039 (Figure 2 - B)Image 290050 (Figure 3 - D)Image 290037 (Figure 2 - A)Image 290085 (Figure 5 - A)Image 290112 (Figure 6 - A)Image 290260 (Figure 4 - A)Image 290104 (Figure 5 - F)Figure 3 - DImage 290108 (Figure 5 - F)Image 290103 (Figure 5 - F)Image 290324 (Figure 6 - B)Image 290086 (Figure 5 - A)Image 290098 (Figure 5 - B)Figure 3 - AImage 290090 (Figure 5 - A)Image 290110 (Figure 5 - F)Image 290119 (Figure 6 - A)Image 290125 (Figure 6 - D)Image 290083 (Figure 5 - A)Image 290074 (Figure 4 - H)Image 290106 (Figure 5 - F)Image 290044 (Figure 2 - D)Figure 5 - AFigure 5 - FImage 290080 (Figure 4 - H)Image 290117 (Figure 6 - A)Figure 5 - BImage 290094 (Figure 5 - B)Figure 5Figure 4Image 290268 (Figure 4 - C)Image 290076 (Figure 4 - H)Image 290088 (Figure 5 - A)Image 290096 (Figure 5 - B)Figure 6Image 290127 (Figure 6 - D)Image 290100 (Figure 5 - B)Figure 3Figure 2Image 290075 (Figure 4 - H)Image 290087 (Figure 5 - A)Image 290111 (Figure 5 - F)Image 290038 (Figure 2 - B)Image 290092 (Figure 5 - B)Image 290318 (Figure 2 - B)Image 290109 (Figure 5 - F)Image 250557 (Figure 2 - A)Image 290130 (Figure 6 - D)Image 290133 (Figure 6 - D)Image 290102 (Figure 5 - F)Image 290078 (Figure 4 - H)Image 290052 (Figure 3 - D)Image 290262 (Figure 4 - A)Image 290273 (Figure 4 - E)Image 290320 (Figure 2 - B)Image 290124 (Figure 6 - D)Image 290105 (Figure 5 - F)Image 290265 (Figure 4 - C)Image 290113 (Figure 6 - A)Image 290099 (Figure 5 - B)Image 290272 (Figure 4 - E)Image 290264 (Figure 4 - C)Figure 2 - DFigure 2 - AImage 290072 (Figure 4 - H)Figure 2 - BImage 290270 (Figure 4 - E)Image 290051 (Figure 3 - D)Image 290259 (Figure 4 - A)Image 290261 (Figure 4 - A)Image 290267 (Figure 4 - C)Figure 4 - AImage 290489 (Figure 2 - B)Image 290079 (Figure 4 - H)Image 290257 (Figure 4 - A)Image 290045 (Figure 3 - A)Figure 4 - HFigure 4 - EFigure 4 - CImage 290093 (Figure 5 - B)Image 290114 (Figure 6 - A)Image 290095 (Figure 5 - B)Image 290089 (Figure 5 - A)Image 290077 (Figure 4 - H)Image 290081 (Figure 4 - H)Image 290321 (Figure 6 - B)Image 290101 (Figure 5 - B)Image 290128 (Figure 6 - D)Image 290132 (Figure 6 - D)Image 290043 (Figure 2 - D)Image 290053 (Figure 3 - D)Image 290256 (Figure 5 - A)Figure 6 - DFigure 6 - AFigure 6 - BImage 290116 (Figure 6 - A)Joachim WittbrodtMaren KlingerHolger LorenzOliver J. GrussIvana CadoKerstin HupfeldDaigo InoueHideki YokoyamaThomas RuppertHerbert SteinbeisserDmytro MayiloStephanie FreissBurkhard HoeckendorfStefanie DraegerSahra PilzFelix BärenzSabine MerkerJustus Tegha-DunghufalseThe centriolar satellite protein SSX2IP promotes centrosome maturation2013-07-01T11:24:52Z2018-11-29T11:24:52Z2018-11-29T11:24:52ZS-JCBD-20130212210.1083/jcb.201302122