{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Jens Tilsner"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201304003"],"attach_to":["JCB"],"legend":["GFP-TGB1 in uninfected tissue co-expressed with unfused TGB2 and TGB3 from separate plasmids. Complete z-stack. Figure 4H corresponds to the maximum projection.","PVX.deltaTGB1.GFP-CP movement complementation by unfused, wild-type TGB1 expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to standardize complementation experiments. Complete z-stack. Figure S3C corresponds to the maximum projection.","Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue also expressing TGB3-TagRFP and unfused TGB1, TGB2, and TGB3. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3M corresponds to the overlay maximum projection.","TGB1[DEY to RRY]-TagRFP in a PVX.GFP-CP infection. Chloroplasts (chlorophyll autofluorescence in blue) shown to highlight plastid-association of TGB1[DEY to RRY] granules. Figure S4C corresponds to the overlay maximum projection.","vRNA (PUM-BiFC) forming whorls\" in caps/VRCs in a PVX.mCherry-CP lesion. Single scan.\"","Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue also expressing TGB3-TagRFP, unfused TGB1, TGB2, and TGB3 and unfused CP. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3N corresponds to the overlay maximum projection.","GFP-TGB2 in uninfected tissue 1 dpi; plasmodesmata stained with aniline blue and shown in red. Single scan.","3D-SIM super-resolution image of GFP-TGB2 caps. GFP-TGB2, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.","A PVX.mCherry-CP lesion entering GFP-TGB2 expressing tissue. Complete z-stack. Figure 2D corresponds to z-section 16.","vRNA (PUM-BiFC) forming whorls\" in a large cap/VRC in a PVX.mCherry-CP lesion. Complete z-stack. Figure 3J corresponds to z-section 9.\"","PVX.deltaTGB1.GFP-CP movement complementation by TagRFP-TGB1 expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TagRFP-TGB1. Complete z-stack. Figure S3E corresponds to the overlay maximum projection.","TGB3-TagRFP and unfused TGB3 in uninfected tissue 2 dpi' plasmodesmata stained with aniline blue and shown in green. Complete z-stack. Figure 4D corresponds to the overlay maximum projection.","TGB1[deltaIV]-GFP in a PVX.mCherry-CP infection. Complete z-stack. Figure S4A corresponds to the overlay maximum projection.","A PVX.mCherry-CP lesion entering TGB1-GFP expressing tissue. Complete z-stack. Figure 2A corresponds to the overlay maximum projection.","TGB1-TagRFP and unfused TGB1 co-expressed in uninfected tissue (3 dpi). Complete z-stack. The insert in Figure S1E corresponds to the maximum projection.","A PVX.GFP-CP lesion entering TGB3-TagRFP expressing tissue.  Complete z-stack. Figure 2H corresponds to the overlay maximum projection.","TGB1-GFP in uninfected tissue co-expressed with unfused TGB2 and TGB3 from a bicistronic plasmid. This mimics the situation in a viral infection and results in a ~10:1 TGB2/TGB3 ratio. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 4G corresponds to the overlay  maximum projection.","Replicase (165k[1-997])-GFP associated with caps in a PVX.mCherry-CP lesion. Callose at plasmodesmata stained with aniline blue. Single scan. Figure 3E corresponds to the 3-colour overlay, Figure 3F to GFP and aniline blue channels only.","TGB1-TagRFP and unfused TGB1 co-expressed in uninfected tissue (3 dpi). Complete z-stack. Figure S1E (main panel) corresponds to the maximum projection.","TGB1-GFP and unfused TGB3 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1I corresponds to the maximum projection.","Replicase (165k[1-997])-GFP associated with caps in a PVX.mCherry-CP lesion; plasmodesmata labelled by mCherry-CP inside the pores. Single scan.","Anti-TGB2 immunogold-EM in PVX.GFP-CP infection. Full TEM image.","PVX.GFP-CP[deltaC10] infection (3 dpi). Complete z-stack. Figure S3A corresponds to the maximum projection.","GFP-CP, TGB1-TagRFP, unfused CP and unfused TGB1, TGB2 and TGB3 co-expressed in uninfected tissue. Plasmodesmata labelled by TGB1-TagRFP. Single scan.","TGB1[GKS to GEA]-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue. The silencing suppressor p19k is also co-expressed to increase expression levels. Complete z-stack. The top panel of Figure S4B corresponds to the maximum projection.","GFP-TGB1 and unfused TGB2 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1F corresponds to the maximum projection.","Leading edge of a PVX.GFP-CP infection. Full TEM image.","TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Single scan.The insert in Figure 6A corresponds to the overlay.","GFP-CP expressed in trans in a PVX.mCherry-CP infection and incorporated into virions alongside virus-expressed mCherry-CP. Complete z-stack. Figure 7I corresponds to the overlay maximum projection.","PVX.GFP-CP[deltaC10] infection. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S3B corresponds to the overlay maximum projection.","TagRFP-TGB2 co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP lesion. Complete z-stack. Figure 2F corresponds to z-section 1.","TGB1-TagRFP in a PVX.GFP-CP[deltaC10] infection. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Single scan corresponds to main panel of Figure 7A.","TGB3-TagRFP cap co-localized with GFP-CP-labelled plasmodesmata in a PVX.GFP-CP lesion. Single scan.","TagRFP-TGB1 co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection site. Single scan.","TGB3-GFP in uninfected tissue 1 dpi. Complete z-stack. Figure S2A corresponds to the maximum projection.","GFP-TGB2 caps co-localized with mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.","Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3L corresponds to the overlay maximum projection.","GFP-CP, unfused CP and unfused TGB1, TGB2 and TGB3 co-expressed in uninfected tissue. The only GFP-CP punctae are cytoplasmic aggregates. Single scan.","PVX.deltaTGB1.GFP-CP and unfused TGB1[R15A] expressed in trans. The silencing suppressor p19k is also co-expressed. Complete z-stack. Both main panel and insert of Figure 7F derive from z-section 2.","GFP-TGB2, TGB1-TagRFP, and unfused TGB2 and TGB3 co-expressed in uninfected tissue. Single scan.","PVX.deltaTGB1.GFP-CP. GFP-CP insertion into plasmodesmata complemented by unfused, wild-type TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed to standardize conditions in all complementation experiments. Complete z-stack. Figure 7B corresponds to z-section 4.","TGB1-GFP in uninfected tissue co-expressed with unfused TGB2 and TGB3 from separate plasmids (resulting in a ~1:1 TGB2/TGB3 ratio). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 4F corresponds to the overlay maximum projection.","PVX.deltaTGB1.GFP-CP and TGB1-TagRFP expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan. Both left and right panels of Figure 7C are derived from this image.","TGB1-TagRFP co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection site. Single scan.","TGB1[R15A]-TagRFP co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection. Single scan.","Replicase (165k[1-997])-GFP forming whorls\" within a VRC in a PVX.mCherry-CP lesion. Single scan.\"","TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Single scan.","PVX.deltaTGB1.GFP-CP movement complementation by TGB1-TagRFP expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TGB1-TagRFP. Complete z-stack. Figure S3D corresponds to the overlay maximum projection.","TGB1-GFP and unfused TGB2 co-expressed in uninfected tissue (2 dpi). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S1H corresponds to the overlay maximum projection.","GFP-CP expressed in trans in a PVX.mCherry-CP infection and inserted into plasmodesmata alongside virus-expressed mCherry-CP. Single scan.","GFP-TGB1 in uninfected tissue 1 dpi. Complete z-stack. Figure S1A corresponds to the maximum projection.","GFP-TGB2 in uninfected tissue 1 dpi. Complete z-stack. Figure 4A corresponds to the maximum projection","TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Complete z-stack. Figure 6A (main panel) corresponds to z-section 12.","3D-SIM super-resolution image of TGB3-GFP caps. TGB3-GFP, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.","PVX.deltaTGB1.GFP-CP and TagRFP-TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan corresponds to Figure 7D (insert).","PVX.deltaTGB1.GFP-CP and TagRFP-TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan corresponds to Figure 7D (main panel).","TGB3-GFP, TGB1-TagRFP, and unfused TGB2 and TGB3 co-expressed in uninfected tissue. Single scan.","GFP-TGB1 and unfused TGB3 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1G corresponds to the maximum projection.","3D-SIM super-resolution image of GFP-TGB2 cap. GFP-TGB2, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.","TGB1[R15A]-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Figure S4D corresponds to the overlay maximum projection.","TGB3-GFP in uninfected tissue 4 dpi. Complete z-stack. Figure S2A corresponds to the maximum projection.","TGB3-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue (3 dpi). Complete z-stack. Figure 4E corresponds to the maximum projection.","TGB1[GKS to GEA]-TagRFP in a PVX.GFP-CP infection. Single scan. The bottom panel of Figure S4B corresponds to the overlay.","vRNA (PUM-BiFC) associated with caps in a PVX.mCherry-CP lesion. Single scan.","GFP-TGB2 in uninfected tissue 2 dpi. Complete z-stack. Figure 4B corresponds to the maximum projection","GFP-TGB1 in uninfected tissue 2 dpi. Complete z-stack. Figure S1A corresponds to the maximum projection.","TGB3-TagRFP in a PVX.GFP-CP[deltaC10] infection. Single scan corresponds to insert in Figure 7A.","PVX.deltaTGB1.GFP-CP movement complementation by TGB1[R15A] expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TGB1[R15A]. Complete z-stack. Figure S3F corresponds to the maximum projection.","GFP-TGB1 and unfused TGB1 co-expressed in uninfected tissue (2 dpi). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S1C corresponds to the overlay maximum projection.","Replicase (165k[1-997])-GFP adjacent to mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.","vRNA (PUM-BiFC) adjacent to mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.","TGB3-TagRFP caps co-localized with GFP-CP-labelled plasmodesmata in a PVX.GFP-CP lesion. Single scan.","TGB1-GFP in uninfected tissue 1 dpi. Complete z-stack. Figure S1D corresponds to the maximum projection."],"repository":["bioimages"],"figure_sub":["Image 291153 (Figure 7 - C)","Image 291158 (Figure 7 - G)","Image 291127 (Figure 3 - G)","Image 291131 (Figure 3 - K)","Image 291141 (Supplemental Figure 4 - A)","Supplemental Figure 2 - A","Image 291081 (Figure 2 - A)","Supplemental Figure 2 - B","Image 291168 (Supplemental Figure 1 - F)","Image 291064 (Figure 4 - H)","Image 291179 (Supplemental Figure 3 - F)","Image 290788 (Figure 4 - B)","Image 291035 (Figure 4 - G)","Image 291144 (Figure 5 - A)","Image 291150 (Figure 7 - A)","Image 291171 (Supplemental Figure 1 - I)","Image 291176 (Supplemental Figure 3 - C)","Image 291151 (Figure 7 - A)","Image 291164 (Supplemental Figure 1 - C)","Figure 3 - D","Image 291114 (Figure 2 - B)","Figure 3 - G","Image 290914 (Figure 4 - E)","Figure 3 - H","Figure 3 - I","Image 291124 (Figure 3 - D)","Figure 3 - J","Figure 3 - K","Figure 3 - A","Figure 3 - B","Image 291178 (Supplemental Figure 3 - E)","Figure 3 - C","Image 291139 (Supplemental Figure 4 - C)","Figure 3 - L","Image 291122 (Figure 3 - A)","Figure 3 - M","Image 291155 (Figure 7 - D)","Figure 3 - N","Image 291162 (Supplemental Figure 1 - A)","Image 291208 (Figure 5 - D)","Image 290774 (Figure 4 - A)","Supplemental Figure 3 - B","Supplemental Figure 3 - C","Figure 5 - A","Supplemental Figure 3 - A","Supplemental Figure 3 - F","Image 291205 (Figure 5 - G)","Supplemental Figure 3 - D","Image 291129 (Figure 3 - I)","Supplemental Figure 3 - E","Figure 5 - F","Image 291146 (Figure 6 - A)","Figure 5 - G","Image 291160 (Figure 7 - I)","Figure 5 - B","Figure 5 - C","Image 291126 (Figure 3 - E and F)","Figure 5 - D","Figure 5 - E","Image 291148 (Figure 6 - B)","Image 291118 (Figure 2 - E)","Image 290910 (Figure 4 - C)","Figure 5","Figure 4","Figure 7","Figure 6","Image 291140 (Supplemental Figure 4 - D)","Image 291134 (Figure 3 - N)","Image 291157 (Figure 7 - F)","Image 291116 (Figure 2 - D)","Figure 3","Figure 2","Supplemental Figure 1 - G","Supplemental Figure 1 - F","Supplemental Figure 1 - I","Supplemental Figure 4","Supplemental Figure 1 - H","Supplemental Figure 1 - C","Supplemental Figure 2","Image 291119 (Figure 2 - H)","Supplemental Figure 1 - B","Supplemental Figure 3","Image 290925 (Figure 4 - F)","Supplemental Figure 1 - E","Supplemental Figure 1","Supplemental Figure 1 - D","Figure 7 - A","Figure 7 - B","Figure 7 - C","Image 291173 (Supplemental Figure 2 - B)","Image 291149 (Figure 5 - B)","Figure 7 - D","Figure 7 - E","Figure 7 - F","Image 291174 (Supplemental Figure 3 - A)","Figure 7 - G","Image 291143 (Supplemental Figure 4 - B)","Figure 7 - H","Figure 7 - I","Figure 7 - J","Image 291154 (Figure 7 - D)","Image 291132 (Figure 3 - L)","Image 291159 (Figure 7 - H)","Image 291128 (Figure 3 - H)","Image 291166 (Supplemental Figure 1 - E)","Image 291117 (Figure 2 - F)","Image 291130 (Figure 3 - J)","Image 291169 (Supplemental Figure 1 - G)","Image 291121 (Figure 2 - J)","Image 291163 (Supplemental Figure 1 - B)","Figure 2 - G","Figure 2 - H","Figure 2 - E","Figure 2 - F","Image 291152 (Figure 7 - B)","Figure 2 - I","Figure 3 - E and F","Figure 2 - J","Supplemental Figure 1 - A","Figure 2 - C","Figure 2 - D","Figure 2 - A","Image 291177 (Supplemental Figure 3 - D)","Figure 2 - B","Image 291167 (Supplemental Figure 1 - E)","Image 291165 (Supplemental Figure 1 - D)","Image 291207 (Figure 5 - C)","Figure 4 - A","Figure 4 - B","Image 291204 (Figure 5 - F)","Image 291123 (Figure 3 - B)","Image 291180 (Supplemental Figure 3 - B)","Image 291138 (Figure 3 - C)","Image 291120 (Figure 2 - I)","Figure 4 - G","Figure 4 - H","Figure 4 - E","Figure 4 - F","Image 291206 (Figure 2 - G)","Figure 4 - C","Image 291170 (Supplemental Figure 1 - H)","Figure 4 - D","Image 291147 (Figure 6 - A)","Image 291142 (Supplemental Figure 4 - B)","Image 291133 (Figure 3 - M)","Image 291156 (Figure 7 - E)","Image 291115 (Figure 2 - C)","Image 291209 (Figure 5 - E)","Supplemental Figure 4 - A","Supplemental Figure 4 - B","Supplemental Figure 4 - C","Supplemental Figure 4 - D","Image 291135 (Figure 4 - D)","Figure 6 - A","Image 291161 (Figure 7 - J)","Figure 6 - B","Image 291172 (Supplemental Figure 2 - A)"],"pubmed_authors":["Olga Linnik","Karl J. Oparka","Jens Tilsner","Ian M. Roberts","Sean N. Chapman","Marion Louveaux"],"additional_accession":[]},"is_claimable":false,"name":"Replication and trafficking of a plant virus are coupled at the entrances of plasmodesmata","description":null,"dates":{"release":"2013-06-24T11:25:07Z","modification":"2018-11-29T11:25:07Z","creation":"2018-11-29T11:25:07Z"},"accession":"S-JCBD-201304003","cross_references":{"doi":["10.1083/jcb.201304003"]}}