bioimagesUnknownJens TilsnerThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201304003JCB

GFP-TGB1 in uninfected tissue co-expressed with unfused TGB2 and TGB3 from separate plasmids. Complete z-stack. Figure 4H corresponds to the maximum projection.

PVX.deltaTGB1.GFP-CP movement complementation by unfused, wild-type TGB1 expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to standardize complementation experiments. Complete z-stack. Figure S3C corresponds to the maximum projection.

Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue also expressing TGB3-TagRFP and unfused TGB1, TGB2, and TGB3. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3M corresponds to the overlay maximum projection.

TGB1[DEY to RRY]-TagRFP in a PVX.GFP-CP infection. Chloroplasts (chlorophyll autofluorescence in blue) shown to highlight plastid-association of TGB1[DEY to RRY] granules. Figure S4C corresponds to the overlay maximum projection.

vRNA (PUM-BiFC) forming whorls" in caps/VRCs in a PVX.mCherry-CP lesion. Single scan."

Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue also expressing TGB3-TagRFP, unfused TGB1, TGB2, and TGB3 and unfused CP. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3N corresponds to the overlay maximum projection.

GFP-TGB2 in uninfected tissue 1 dpi; plasmodesmata stained with aniline blue and shown in red. Single scan.

3D-SIM super-resolution image of GFP-TGB2 caps. GFP-TGB2, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.

A PVX.mCherry-CP lesion entering GFP-TGB2 expressing tissue. Complete z-stack. Figure 2D corresponds to z-section 16.

vRNA (PUM-BiFC) forming whorls" in a large cap/VRC in a PVX.mCherry-CP lesion. Complete z-stack. Figure 3J corresponds to z-section 9."

PVX.deltaTGB1.GFP-CP movement complementation by TagRFP-TGB1 expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TagRFP-TGB1. Complete z-stack. Figure S3E corresponds to the overlay maximum projection.

TGB3-TagRFP and unfused TGB3 in uninfected tissue 2 dpi' plasmodesmata stained with aniline blue and shown in green. Complete z-stack. Figure 4D corresponds to the overlay maximum projection.

TGB1[deltaIV]-GFP in a PVX.mCherry-CP infection. Complete z-stack. Figure S4A corresponds to the overlay maximum projection.

A PVX.mCherry-CP lesion entering TGB1-GFP expressing tissue. Complete z-stack. Figure 2A corresponds to the overlay maximum projection.

TGB1-TagRFP and unfused TGB1 co-expressed in uninfected tissue (3 dpi). Complete z-stack. The insert in Figure S1E corresponds to the maximum projection.

A PVX.GFP-CP lesion entering TGB3-TagRFP expressing tissue. Complete z-stack. Figure 2H corresponds to the overlay maximum projection.

TGB1-GFP in uninfected tissue co-expressed with unfused TGB2 and TGB3 from a bicistronic plasmid. This mimics the situation in a viral infection and results in a ~10:1 TGB2/TGB3 ratio. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 4G corresponds to the overlay maximum projection.

Replicase (165k[1-997])-GFP associated with caps in a PVX.mCherry-CP lesion. Callose at plasmodesmata stained with aniline blue. Single scan. Figure 3E corresponds to the 3-colour overlay, Figure 3F to GFP and aniline blue channels only.

TGB1-TagRFP and unfused TGB1 co-expressed in uninfected tissue (3 dpi). Complete z-stack. Figure S1E (main panel) corresponds to the maximum projection.

TGB1-GFP and unfused TGB3 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1I corresponds to the maximum projection.

Replicase (165k[1-997])-GFP associated with caps in a PVX.mCherry-CP lesion; plasmodesmata labelled by mCherry-CP inside the pores. Single scan.

Anti-TGB2 immunogold-EM in PVX.GFP-CP infection. Full TEM image.

PVX.GFP-CP[deltaC10] infection (3 dpi). Complete z-stack. Figure S3A corresponds to the maximum projection.

GFP-CP, TGB1-TagRFP, unfused CP and unfused TGB1, TGB2 and TGB3 co-expressed in uninfected tissue. Plasmodesmata labelled by TGB1-TagRFP. Single scan.

TGB1[GKS to GEA]-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue. The silencing suppressor p19k is also co-expressed to increase expression levels. Complete z-stack. The top panel of Figure S4B corresponds to the maximum projection.

GFP-TGB1 and unfused TGB2 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1F corresponds to the maximum projection.

Leading edge of a PVX.GFP-CP infection. Full TEM image.

TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Single scan.The insert in Figure 6A corresponds to the overlay.

GFP-CP expressed in trans in a PVX.mCherry-CP infection and incorporated into virions alongside virus-expressed mCherry-CP. Complete z-stack. Figure 7I corresponds to the overlay maximum projection.

PVX.GFP-CP[deltaC10] infection. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S3B corresponds to the overlay maximum projection.

TagRFP-TGB2 co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP lesion. Complete z-stack. Figure 2F corresponds to z-section 1.

TGB1-TagRFP in a PVX.GFP-CP[deltaC10] infection. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Single scan corresponds to main panel of Figure 7A.

TGB3-TagRFP cap co-localized with GFP-CP-labelled plasmodesmata in a PVX.GFP-CP lesion. Single scan.

TagRFP-TGB1 co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection site. Single scan.

TGB3-GFP in uninfected tissue 1 dpi. Complete z-stack. Figure S2A corresponds to the maximum projection.

GFP-TGB2 caps co-localized with mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.

Replicase (165k[1-997])-GFP confined to nuclei in uninfected tissue. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 3L corresponds to the overlay maximum projection.

GFP-CP, unfused CP and unfused TGB1, TGB2 and TGB3 co-expressed in uninfected tissue. The only GFP-CP punctae are cytoplasmic aggregates. Single scan.

PVX.deltaTGB1.GFP-CP and unfused TGB1[R15A] expressed in trans. The silencing suppressor p19k is also co-expressed. Complete z-stack. Both main panel and insert of Figure 7F derive from z-section 2.

GFP-TGB2, TGB1-TagRFP, and unfused TGB2 and TGB3 co-expressed in uninfected tissue. Single scan.

PVX.deltaTGB1.GFP-CP. GFP-CP insertion into plasmodesmata complemented by unfused, wild-type TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed to standardize conditions in all complementation experiments. Complete z-stack. Figure 7B corresponds to z-section 4.

TGB1-GFP in uninfected tissue co-expressed with unfused TGB2 and TGB3 from separate plasmids (resulting in a ~1:1 TGB2/TGB3 ratio). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure 4F corresponds to the overlay maximum projection.

PVX.deltaTGB1.GFP-CP and TGB1-TagRFP expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan. Both left and right panels of Figure 7C are derived from this image.

TGB1-TagRFP co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection site. Single scan.

TGB1[R15A]-TagRFP co-localized with GFP-CP inside plasmodesmata in a PVX.GFP-CP infection. Single scan.

Replicase (165k[1-997])-GFP forming whorls" within a VRC in a PVX.mCherry-CP lesion. Single scan."

TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Single scan.

PVX.deltaTGB1.GFP-CP movement complementation by TGB1-TagRFP expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TGB1-TagRFP. Complete z-stack. Figure S3D corresponds to the overlay maximum projection.

TGB1-GFP and unfused TGB2 co-expressed in uninfected tissue (2 dpi). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S1H corresponds to the overlay maximum projection.

GFP-CP expressed in trans in a PVX.mCherry-CP infection and inserted into plasmodesmata alongside virus-expressed mCherry-CP. Single scan.

GFP-TGB1 in uninfected tissue 1 dpi. Complete z-stack. Figure S1A corresponds to the maximum projection.

GFP-TGB2 in uninfected tissue 1 dpi. Complete z-stack. Figure 4A corresponds to the maximum projection

TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in a transgenic plant expressing ER-lumenal GFP (GFP-HDEL). Complete z-stack. Figure 6A (main panel) corresponds to z-section 12.

3D-SIM super-resolution image of TGB3-GFP caps. TGB3-GFP, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.

PVX.deltaTGB1.GFP-CP and TagRFP-TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan corresponds to Figure 7D (insert).

PVX.deltaTGB1.GFP-CP and TagRFP-TGB1 expressed in trans. The silencing suppressor p19k is also co-expressed. Single scan corresponds to Figure 7D (main panel).

TGB3-GFP, TGB1-TagRFP, and unfused TGB2 and TGB3 co-expressed in uninfected tissue. Single scan.

GFP-TGB1 and unfused TGB3 co-expressed in uninfected tissue (2 dpi). Complete z-stack. Figure S1G corresponds to the maximum projection.

3D-SIM super-resolution image of GFP-TGB2 cap. GFP-TGB2, TGB1-TagRFP and unfused TGB2 and TGB3 co-expressed in uninfected tissue. 3D-SIM-deconvolved region of interest only.

TGB1[R15A]-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue. Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Figure S4D corresponds to the overlay maximum projection.

TGB3-GFP in uninfected tissue 4 dpi. Complete z-stack. Figure S2A corresponds to the maximum projection.

TGB3-TagRFP co-expressed with unfused TGB2 and TGB3 in uninfected tissue (3 dpi). Complete z-stack. Figure 4E corresponds to the maximum projection.

TGB1[GKS to GEA]-TagRFP in a PVX.GFP-CP infection. Single scan. The bottom panel of Figure S4B corresponds to the overlay.

vRNA (PUM-BiFC) associated with caps in a PVX.mCherry-CP lesion. Single scan.

GFP-TGB2 in uninfected tissue 2 dpi. Complete z-stack. Figure 4B corresponds to the maximum projection

GFP-TGB1 in uninfected tissue 2 dpi. Complete z-stack. Figure S1A corresponds to the maximum projection.

TGB3-TagRFP in a PVX.GFP-CP[deltaC10] infection. Single scan corresponds to insert in Figure 7A.

PVX.deltaTGB1.GFP-CP movement complementation by TGB1[R15A] expressed in trans (3 dpi). Silencing suppressor p19k is also expressed to compensate for potential reduced suppressor activity of TGB1[R15A]. Complete z-stack. Figure S3F corresponds to the maximum projection.

GFP-TGB1 and unfused TGB1 co-expressed in uninfected tissue (2 dpi). Chloroplasts (chlorophyll autofluorescence in blue) shown for contrast. Complete z-stack. Figure S1C corresponds to the overlay maximum projection.

Replicase (165k[1-997])-GFP adjacent to mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.

vRNA (PUM-BiFC) adjacent to mCherry-CP-labelled plasmodesmata in a PVX.mCherry-CP lesion. Single scan.

TGB3-TagRFP caps co-localized with GFP-CP-labelled plasmodesmata in a PVX.GFP-CP lesion. Single scan.

TGB1-GFP in uninfected tissue 1 dpi. Complete z-stack. Figure S1D corresponds to the maximum projection.

bioimagesImage 291153 (Figure 7 - C)Image 291158 (Figure 7 - G)Image 291127 (Figure 3 - G)Image 291131 (Figure 3 - K)Image 291141 (Supplemental Figure 4 - A)Supplemental Figure 2 - AImage 291081 (Figure 2 - A)Supplemental Figure 2 - BImage 291168 (Supplemental Figure 1 - F)Image 291064 (Figure 4 - H)Image 291179 (Supplemental Figure 3 - F)Image 290788 (Figure 4 - B)Image 291035 (Figure 4 - G)Image 291144 (Figure 5 - A)Image 291150 (Figure 7 - A)Image 291171 (Supplemental Figure 1 - I)Image 291176 (Supplemental Figure 3 - C)Image 291151 (Figure 7 - A)Image 291164 (Supplemental Figure 1 - C)Figure 3 - DImage 291114 (Figure 2 - B)Figure 3 - GImage 290914 (Figure 4 - E)Figure 3 - HFigure 3 - IImage 291124 (Figure 3 - D)Figure 3 - JFigure 3 - KFigure 3 - AFigure 3 - BImage 291178 (Supplemental Figure 3 - E)Figure 3 - CImage 291139 (Supplemental Figure 4 - C)Figure 3 - LImage 291122 (Figure 3 - A)Figure 3 - MImage 291155 (Figure 7 - D)Figure 3 - NImage 291162 (Supplemental Figure 1 - A)Image 291208 (Figure 5 - D)Image 290774 (Figure 4 - A)Supplemental Figure 3 - BSupplemental Figure 3 - CFigure 5 - ASupplemental Figure 3 - ASupplemental Figure 3 - FImage 291205 (Figure 5 - G)Supplemental Figure 3 - DImage 291129 (Figure 3 - I)Supplemental Figure 3 - EFigure 5 - FImage 291146 (Figure 6 - A)Figure 5 - GImage 291160 (Figure 7 - I)Figure 5 - BFigure 5 - CImage 291126 (Figure 3 - E and F)Figure 5 - DFigure 5 - EImage 291148 (Figure 6 - B)Image 291118 (Figure 2 - E)Image 290910 (Figure 4 - C)Figure 5Figure 4Figure 7Figure 6Image 291140 (Supplemental Figure 4 - D)Image 291134 (Figure 3 - N)Image 291157 (Figure 7 - F)Image 291116 (Figure 2 - D)Figure 3Figure 2Supplemental Figure 1 - GSupplemental Figure 1 - FSupplemental Figure 1 - ISupplemental Figure 4Supplemental Figure 1 - HSupplemental Figure 1 - CSupplemental Figure 2Image 291119 (Figure 2 - H)Supplemental Figure 1 - BSupplemental Figure 3Image 290925 (Figure 4 - F)Supplemental Figure 1 - ESupplemental Figure 1Supplemental Figure 1 - DFigure 7 - AFigure 7 - BFigure 7 - CImage 291173 (Supplemental Figure 2 - B)Image 291149 (Figure 5 - B)Figure 7 - DFigure 7 - EFigure 7 - FImage 291174 (Supplemental Figure 3 - A)Figure 7 - GImage 291143 (Supplemental Figure 4 - B)Figure 7 - HFigure 7 - IFigure 7 - JImage 291154 (Figure 7 - D)Image 291132 (Figure 3 - L)Image 291159 (Figure 7 - H)Image 291128 (Figure 3 - H)Image 291166 (Supplemental Figure 1 - E)Image 291117 (Figure 2 - F)Image 291130 (Figure 3 - J)Image 291169 (Supplemental Figure 1 - G)Image 291121 (Figure 2 - J)Image 291163 (Supplemental Figure 1 - B)Figure 2 - GFigure 2 - HFigure 2 - EFigure 2 - FImage 291152 (Figure 7 - B)Figure 2 - IFigure 3 - E and FFigure 2 - JSupplemental Figure 1 - AFigure 2 - CFigure 2 - DFigure 2 - AImage 291177 (Supplemental Figure 3 - D)Figure 2 - BImage 291167 (Supplemental Figure 1 - E)Image 291165 (Supplemental Figure 1 - D)Image 291207 (Figure 5 - C)Figure 4 - AFigure 4 - BImage 291204 (Figure 5 - F)Image 291123 (Figure 3 - B)Image 291180 (Supplemental Figure 3 - B)Image 291138 (Figure 3 - C)Image 291120 (Figure 2 - I)Figure 4 - GFigure 4 - HFigure 4 - EFigure 4 - FImage 291206 (Figure 2 - G)Figure 4 - CImage 291170 (Supplemental Figure 1 - H)Figure 4 - DImage 291147 (Figure 6 - A)Image 291142 (Supplemental Figure 4 - B)Image 291133 (Figure 3 - M)Image 291156 (Figure 7 - E)Image 291115 (Figure 2 - C)Image 291209 (Figure 5 - E)Supplemental Figure 4 - ASupplemental Figure 4 - BSupplemental Figure 4 - CSupplemental Figure 4 - DImage 291135 (Figure 4 - D)Figure 6 - AImage 291161 (Figure 7 - J)Figure 6 - BImage 291172 (Supplemental Figure 2 - A)Olga LinnikKarl J. OparkaJens TilsnerIan M. RobertsSean N. ChapmanMarion LouveauxfalseReplication and trafficking of a plant virus are coupled at the entrances of plasmodesmata2013-06-24T11:25:07Z2018-11-29T11:25:07Z2018-11-29T11:25:07ZS-JCBD-20130400310.1083/jcb.201304003