bioimagesUnknownHenning F. HornThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201304004JCB

Low magnification view of a testis section from a wild-type mouse stained with anti-gH2AX (red) and DAPI (blue).

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. A projection of the original stack is shown here.

Wild-type zygotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. The original stack is shown here.

Spermatocyte spreads from Kash5-null mice were immunostained with anti-SCP3 (red) and anti-SUN1 (green). A projection of the original stack is shown here.

Wild-type pachytene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. A projection of the original stack is shown here.

Spermatocyte spreads were immunostained for KASH5 (green) and SCP3 (red). Samples were imaged by conventional deconvolution microscopy. The original stack is shown here.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (red) and anti-SUN1 (green). The original stack is shown here.

Wild-type spermatocyte spread immunostained with anti-Sun1 (green) and anti-Dynein (red). DAPI is shown in blue. This image corresponds to bottom row of images.

Wild-type zygotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. A projection of the original stack is shown here.

Testes sections from KASH5-null mice were immunostained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualized using a Zeiss LSM510 confocal microscope. DNA is labeled in blue.

Immunoblot of testes tissue lysates were probed with anti-LaminB1 as a loading control .Lanes 2, 3, and 4 represent wild-type, Kash5-het, and Kash5-null samples.

Wild-type spermatocyte spreads were immunostained with anti-Sun1 (red) antibody and labeled for telomeres using Tel-FISH (green). DNA is labeled in blue.

Spermatocyte spreads from wild-type mice were immuonstained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using the DeltaVision deconvolution microscope. The majority of SCP3-positive cells are in the pachytene stage and have resolved most of the Rad51 foci. These images are after deconvolution.

Spermatocyte spreads derived from KASH5-null mice were immunstained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualised using structured illumination microscopy. The full stack is shown here.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. The original stack is shown here.

Spermatocyte spreads from Kash5-null mice were immunostained with anti-SCP3 (red) and anti-SUN1 (green). The original stack is shown here.

Kash5-null spermatocyte spread immunostained with anti-Sun1 (green) and anti-Dynein (red). DAPI is shown in blue.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using a DeltaVision deconvolution microscope. In spreads from wild-type animals, only the occasional cell is positive for both proteins, which corresponds to the cells in leptotene/zygotene stage. These images are prior to deconvolution.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (red) and anti-SUN1 (green). A projection of the original stack is shown here.

Low magnification view of a testis sections from a KASH5-null mouse stained with anti-gH2AX (red) and DAPI (blue).

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. The original stack is shown here.

Wild-type zygotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. The original stack is shown here.

Testes sections from wild-type mice were immunostained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualized using a Zeiss LSM510 confocal microscope. DNA is labeled in blue.

Spermatocyte spreads from wild-type mice were immuonstained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using the DeltaVision deconvolution microscope. The majority of SCP3-positive cells are in the pachytene stage and have resolved most of the Rad51 foci. These images are prior to deconvolution.

Wild-type pachytene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. The original stack is shown here.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. The original stack is shown here.

Spermatocyte spreads from KASH5-null mice were immunostained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using a DeltaVision deconvolution microscope. These images are after deconvolution.

Wild-type spermatocyte spread immunostained with anti-Sun1 (green) and anti-Dynein (red). DAPI is shown in blue. This image corresponds to top row of images.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. A projection of the original stack is shown here.

Wild-type pachytene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. The original stack is shown here.

Immunoblot of testes tissue lysates were probed with anti-KASH5 antibody. Lanes 2, 3, and 4 represent wild-type, Kash5-het, and Kash5-null samples.

High magnification view of a testis section from a KASH5-null mouse stained with anti-gH2AX (red) and DAPI (blue).

Spermatocyte spreads were immunostained for KASH5 (green) and SCP3 (red). Samples were imaged by conventional deconvolution microscopy. A projection of the original stack is shown here.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. A projection of the original stack is shown here.

Spermatocyte spreads derived from KASH5-null mice were immunstained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualised using structured illumination microscopy. A projection of the full stack is shown here.

Wild-type zygotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. A projection of the original stack is shown here.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by deconvolution microscopy. A projection of the original stack is shown here.

Wild-type testes section immunostained with anti-SCP3 (red) and anti-centromere CREST (green) auto-antibody. DNA is stained in blue.

Spermatocyte spreads were immunostained with anti-KASH5 (green) and anti-SCP3 (red) and visualized using structured illumination microscopy. A projection of the full stack is shown here.

Spermatocyte spreads were immunostained with anti-KASH5 (green) and anti-SCP3 (red) and visualized using structured illumination microscopy. The full stack is shown here.

High magnification view of a testis section from a wild-type mouse stained with anti-gH2AX (red) and DAPI (blue).

Wild-type spermatocytes stained with anti-SCP1 (green) and anti-SCP3 (red). A projection of the full stack is shown here.

Wild-type pachytene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. A projection of the original stack is shown here.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using a DeltaVision deconvolution microscope. In spreads from wild-type animals, only the occasional cell is positive for both proteins, which corresponds to the cells in leptotene/zygotene stage. These images are after deconvolution.

Spermatocyte spreads derived from wild-type mice were immunstained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualised using structured illumination microscopy. A full stack is shown here.

Kash5-null testes section immunostained with anti-SCP3 ((red) and anti-centromere CREST (green) auto-antibody. DNA is labeled in blue.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (red) and anti-KASH5 (green). A projection of the original stack is shown here.

Spermatocyte spreads from wild-type mice were immunostained with anti-SCP3 (red) and anti-KASH5 (green). The original stack is shown here.

Spermatocyte spreads from KASH5-null mice were immunostained with anti-SCP3 (green) and anti-Rad51 (red) antibodies and visualized using a DeltaVision deconvolution microscope. These images are prior to deconvolution.

Spermatocyte spreads derived from wild-type mice were immunstained with anti-SCP1 (green) and anti-SCP3 (red) antibodies and visualised using structured illumination microscopy. A projection of the full stack is shown here.

Kash5-null spermatocyte spreads were immunostained with anti-Sun1 (red) antibody and labeled for telomeres using Tel-FISH (green). DNA is labeled in blue.

Wild-type leptotene spermatocyte stained with anti-KASH5 (green) and anti-SCP3 (red). Images were obtained by structured illumination microscopy. The original stack is shown here.

Wild-type spermatocytes stained with anti-SCP1 (green) and anti-SCP3 (red). The full stack is shown here.

bioimagesImage 292609 (Figure 5 - B)Supplemental Figure 3 - D'Supplemental Figure 5Figure 8 - AFigure 8 - BSupplemental Figure 3Image 292365 (Supplemental Figure 3 - D)Image 292622 (Supplemental Figure 3 - C')Figure 7 - AImage 292322 (Figure 5 - C)Figure 7 - BImage 292630 (Supplemental Figure 5 - None)Image 292332 (Figure 6 - A)Image 292615 (Figure 7 - B)Image 292611 (Figure 5 - B)Image 292333 (Figure 6 - B)Image 292623 (Supplemental Figure 3 - C')Image 292628 (Supplemental Figure 3 - F')Image 292632 (Figure 7 - A)Image 292353 (Figure 8 - A)Supplemental Figure 3 - E'Image 292644 (Figure 5 - B)Supplemental Figure 3 - A'Image 292372 (Supplemental Figure 3 - F)Image 292629 (Supplemental Figure 3 - F')Image 292361 (Supplemental Figure 3 - C)Image 292643 (Figure 4 - D)Image 292357 (Supplemental Figure 3 - B)Image 292624 (Supplemental Figure 3 - D')Image 292352 (Figure 8 - A)Image 292621 (Supplemental Figure 3 - B')Image 292319 (Figure 5 - A)Image 292617 (Figure 7 - B)Image 292368 (Supplemental Figure 3 - E)Image 292631 (Supplemental Figure 5 - None)Image 292619 (Supplemental Figure 3 - A')Image 292616 (Figure 7 - B)Supplemental Figure 5 - NoneImage 292642 (Figure 4 - D)Figure 3 - DImage 292331 (Figure 6 - A)Supplemental Figure 3 - F'Image 292321 (Figure 5 - C)Image 292627 (Supplemental Figure 3 - E')Supplemental Figure 3 - B'Image 292334 (Figure 6 - B)Image 292634 (Figure 7 - A)Image 292354 (Figure 8 - B)Figure 3 - CImage 292320 (Figure 5 - A)Image 292639 (Figure 6 - D)Image 292364 (Supplemental Figure 3 - D)Image 292373 (Supplemental Figure 3 - F)Image 292614 (Figure 7 - B)Image 292318 (Figure 3 - C)Figure 4 - DImage 292317 (Figure 3 - C)Supplemental Figure 3 - BImage 292369 (Supplemental Figure 3 - E)Image 292638 (Figure 6 - D)Supplemental Figure 3 - CFigure 5 - AImage 292610 (Figure 5 - B)Supplemental Figure 3 - AImage 292641 (Figure 6 - D)Supplemental Figure 3 - FImage 292608 (Figure 3 - D)Supplemental Figure 3 - C'Image 292413 (Supplemental Figure 3 - A)Supplemental Figure 3 - DSupplemental Figure 3 - EImage 292412 (Supplemental Figure 3 - A)Image 292618 (Supplemental Figure 3 - A')Figure 5 - BFigure 5 - CImage 292637 (Figure 6 - D)Image 292613 (Figure 7 - B)Image 292607 (Figure 3 - D)Figure 5Image 292612 (Figure 7 - B)Figure 4Image 292640 (Figure 6 - D)Figure 7Figure 6Figure 8Image 292626 (Supplemental Figure 3 - E')Image 292360 (Supplemental Figure 3 - C)Figure 6 - DFigure 6 - AFigure 6 - BImage 292625 (Supplemental Figure 3 - D')Image 292356 (Supplemental Figure 3 - B)Figure 3Image 292636 (Figure 6 - D)Image 292620 (Supplemental Figure 3 - B')Brian BurkeDae In KimKyle J. RouxEsther Sook Miin WongHenning F. HornColin L. StewartGraham D. WrightfalseA mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton2013-09-23T11:25:09Z2018-11-29T11:25:09Z2018-11-29T11:25:09ZS-JCBD-20130400410.1083/jcb.201304004