bioimagesUnknownPeng XuThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201305094JCB

Gcs1(186-352)-GFP localization in fab1Δ cells deficient in PI3,5P2.

GFP-tagged Gcs1 (green) expressed in wild-type cells were imaged relative to the early endosome marker mCherry-Tlg1 (red).

Gcs1-GFP (green) localization in wild-type cells relative to the early endosome marker mCherry-Tlg1 (red).

Gcs1(186-285)-R199Q-GFP (green) expressed in wild-type cells and imaged relative to mCherry-Tlg1 (red).

Time-lapse images of Gcs1-GFP expressed on 2μ plasmids, which exist in multiple copies per cell, in drs2 ts as cells warmed from room temperature to approximately 37 °C on a heated stage.

Gcs1(186-285)-GFP (green) expressed in wild-type cells and imaged relative to mCherry-Tlg1 (red).

Gcs1(186-352)-GFP (green) colocalized to the punctae with the PS biosensor mRFP-Lact-C2 (red).

drs2Δ cells transformed with 2μ Osh4-YFP plasmids (yellow) were imaged when grown in mid-log phase (DIC, gray).

Time-lapse images of Gcs1-GFP expressed on 2μ plasmids, which exist in multiple copies per cell, in wild-type as cells warmed from room temperature to approximately 37 °C on a heated stage.

GFP-tagged Gcs1-L246D (green) expressed in wild-type cells were imaged relative to the early endosome marker mCherry-Tlg1 (red).

GFP-tagged Gcs1(186-352) (green) expressed in wild-type cells were imaged relative to the early endosome marker mCherry-Tlg1 (red).

Gcs1(186-352)-GFP localization in vps34Δ cells deficient in PI3P..

Gcs1(186-352)-GFP (green) colocalized to the punctae with the PS biosensor mRFP-Lact-C2 (red). This image is not shown in the paper.

Wild-type cells transformed with 2μ Osh4-YFP plasmids (yellow) were imaged when grown in mid-log phase (DIC, gray).

Gcs1(186-285)K210Q-GFP (green) expressed in wild-type cells and imaged relative to mCherry-Tlg1 (red).

Wild-type cells transformed with Gcs1(186-352)-GFP and grown to mid-log phase were imaged using a DeltaVision Elite workstation (Applied Precision Instruments, Seattle). Frames were taken very 170 ms for several seconds (see Methods for more details on image acquisition).

Gcs1-GFP (green) localization in drs2∆ cells relative to the early endosome marker mCherry-Tlg1 (red).

Gcs1-GFP localization (green) in snx4∆ cells relative to the early endosome marker mCherry-Tlg1 (red).

bioimagesImage 291954 (Figure 2 - F)Image 292704 (Figure 4 - C)Image 291948 (Figure 2 - C)Image 291951 (Figure 2 - E)Image 291952 (Figure 2 - E)Image 290771 (Figure 1 - C)Image 290832 (Figure 1 - D)Image 291947 (Figure 2 - C)Image 291949 (Figure 2 - D)Image 291953 (Figure 2 - F)Image 292703 (Figure 4 - C)Image 291946 (Figure 2 - C)Figure 4 - CImage 292670 (Figure 1 - A)Figure 1 - AImage 290773 (Figure 1 - C)Figure 1 - DFigure 1 - CImage 290756 (Figure 1 - A)Figure 4Figure 2 - EFigure 2 - FImage 290768 (Figure 1 - C)Image 291950 (Figure 2 - D)Figure 1Figure 2 - CFigure 2 - DImage 290755 (Figure 1 - A)Figure 2Peng XuChristopher G. BurdRichard J. ChiTodd R. GrahamRyan D. BaldridgefalsePhosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport2013-09-09T11:25:23Z2018-11-29T11:25:23Z2018-11-29T11:25:23ZS-JCBD-20130509410.1083/jcb.201305094