{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Marta N. Shahbazi"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201306019"],"attach_to":["JCB"],"legend":["Mouse keratinocytes were isolated from the backskin of p120 conditional knockout mice and grown in culture. After treatment with calcium for 6 h, cells were fixed and immunostained for ECadherin (red), CLASP2 (green), and p120 (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","CLASP2 localization was assessed in primary mouse keratinocytes treated with a scramble RNAi construct under low calcium conditions. Cells were immunostained for CLASP2 (red), α-tubulin (green), and DAPI (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were treated with calcium for 6 h and stained for CLASP2 (red), p120 (green), and DNA (DAPI, blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","p120-null (p120 Δ/Δ) mouse keratinocytes were transfected with p120ΔN-HA. Cells were treated with calcium for 6 h and immunostained for CLASP2 (red), p120 (green) and HA (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","p120-null (p120 Δ/Δ) mouse keratinocytes were treated with calcium for 6 h and immunostained for CLASP2 (red), p120 (green), ECadherin (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were stained for CLASP2 (red), ECadherin (green) and α-tubulin (blue) under normal calcium conditions. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Sections from the backskin of newborn wild-type mice were immunostained for or CLASP2 (red) together with p120 (green), DAPI (blue), and Integrin-β4 (white). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were treated with calcium for 24 h to allow the formation of differentiated suprabasal-like cells in vitro. Cells were immunostained for Nezha (red) together with ECadherin (green) and p120 (blue). In order to distinguish between basal and suprabasal cell-cell contacts, three different planes in the Z axis were imaged with a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were stained for CLASP2 (red), ECadherin (green) and α-tubulin (blue) under low calcium conditions the absence or presence of calcium. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Shown is a time-lapse microscopy video of the dynamic behavior of CLASP2 at cell-cell contacts. Wild-type primary mouse keratinocytes were transfected with plasmids encoding GFP-CLASP2 (green) and p120-cherry (red) and treated with calcium for 4-6 h. The dynamic behavior of both proteins at cell-cell contacts was analyzed by time-lapse microscopy. Images were taken at 37 ºC at intervals of 2 s in a TCS-SP5 (AOBS) laser scanning confocal microscope (Leica Microsystems) with a water immersion objective 63X HCX PL APO 1.2 N.A. using a temperature-controlled incubator chamber in the presence of CO2.","Wild-type (p120 f/f) keratinocytes were treated with calcium for 6 h and immunostained for CLASP2 (red), p120 (green), ECadherin (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Sections from the backskin of newborn wild-type mice were immunostained for Nezha (red) together with p120 (green), DAPI (blue), and Integrin-β4 (white). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were treated with calcium for 10 h following depolymerization of the microtubule network with a 30 µM nocodazole treatment for 2 h. Cells were immunostained for ECadherin (red), CLASP2 (green) and α-tubulin (blue).  Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","CLASP2 expression was downregulated in primary mouse keratinocytes with lentiviruses expressing a specific shRNA. Cells were immunostained for CLASP2 (red), α-tubulin (green), and DAPI (blue) under low calcium conditions. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were treated with calcium for 10 h following depolymerization of the microtubule network with a 30 µM nocodazole treatment for 4 h. Cells were immunostained for ECadherin (red), CLASP2 (green) and α-tubulin (blue).  Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were incubated with protein-A polystyrene beads coated with IgGs as a negative control for 5 h in the presence of calcium. Images show the localization of CLASP2 (red), p120 (green), DAPI (blue) and ECadherin (white). Brightfield images show the presence of the beads. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Mouse keratinocytes were isolated from the backskin of wild-type mice and grown in culture. After treatment with calcium for 6 h, cells were fixed and immunostained for ECadherin (red), CLASP2 (green), and p120 (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were treated with calcium for 24 h to allow the formation of differentiated suprabasal-like cells in vitro. Cells were immunostained for CLASP2 (red) together with ECadherin (green), and p120 (blue). In order to distinguish between basal and suprabasal cell-cell contacts, three different planes in the Z axis were imaged with a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Primary mouse keratinocytes were incubated with protein-A polystyrene beads coated with recombinant Fc-ECadherin for 5 h in the presence of calcium. Images show the localization of CLASP2 (red), p120 (green), DAPI (blue) and ECadherin (white). Brightfield images show the presence of the beads. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","CLASP2 localization was assessed in primary mouse keratinocytes treated with a scramble RNAi construct under normal calcium conditions. Cells were immunostained for CLASP2 (red), α-tubulin (green), and DAPI (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","Control primary mouse keratinocytes were treated with calcium for 10 h. Cells were immunostained for ECadherin (red), CLASP2 (green) and α-tubulin (blue).  Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","CLASP2 expression was downregulated in primary mouse keratinocytes with lentiviruses expressing a specific shRNA. Cells were immunostained for CLASP2 (red), α-tubulin (green), and DAPI (blue) in the absence or presence of calcium. Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope.","p120-null (p120 Δ/Δ) mouse keratinocytes were transfected with p120FL-HA. Cells were treated with calcium for 6 h and immunostained for CLASP2 (red), p120 (green) and HA (blue). Images were taken on a Leica TCS-SP5 (AOBS) laser scanning confocal microscope."],"repository":["bioimages"],"figure_sub":["Image 575516 (Figure  8 - A)","Figure  4 - C","Image 575513 (Figure  5 - A)","Image 575501 (Figure 1 - A)","Image 575503 (Figure 1 - B)","Image 575504 (Figure 1 - F)","Figure  3 - A","Image 575507 (Figure  3 - A)","Video 1 - None","Image 575522 (Figure  4 - C)","Image 575508 (Figure  3 - E)","Image 575502 (Figure 1 - A)","Image 575514 (Figure  5 - A)","Image 575518 (Figure  8 - D)","Image 575517 (Figure  8 - A)","Video 1","Image 575509 (Figure  3 - E)","Image 575524 (Video 1 - None)","Figure  3","Image 575506 (Figure  3 - A)","Image 575510 (Figure  3 - E)","Image 575519 (Figure  8 - D)","Figure 1 - F","Image 575520 (Figure  4 - C)","Figure 1 - A","Figure  5 - A","Image 575512 (Figure  5 - A)","Image 575515 (Figure  5 - A)","Figure 1 - B","Figure  3 - E","Image 575511 (Figure  3 - E)","Image 575505 (Figure 1 - F)","Figure  8 - D","Figure  8","Figure 1","Figure  5","Figure  8 - A","Figure  4","Image 575521 (Figure  4 - C)"],"pubmed_authors":["Elaine Fuchs","Marta N. Shahbazi","Anna Akhmanova","Diego Megias","Gregg G. Gundersen","Carolina Epifano","Mirna Perez-Moreno"],"additional_accession":[]},"is_claimable":false,"name":"CLASP2 interacts with p120-catenin and governs microtubule dynamics at adherens junctions","description":null,"dates":{"release":"2013-12-23T11:25:27Z","modification":"2018-11-29T11:25:27Z","creation":"2018-11-29T11:25:27Z"},"accession":"S-JCBD-201306019","cross_references":{"doi":["10.1083/jcb.201306019"]}}