bioimagesUnknownLena HolembowskiThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201306066JCB

Electron microscopy of a TAp73 knockout mouse testis is shown. Knockout mice show defective cell-cell junctions of the blood-testis barrier on a large scale. Junctions show misplaced and disorderly arrangements of thinned actin bundles, losing the aligned opposing position and orientation. Instead, actin bundles appear ‘offset’.

Electron microscopy of adult testis of a TAp73 knockout mouse is shown. In knockout seminiferous epithelium sperm cells are very loosely packed, and Sertoli cells fail to envelope and retain the developing gametes.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult p73 knockout mouse testis is shown. In knockout testis late-stage developing immature sperm cells fail to properly attach to each other and to Sertoli cells, hence cells lose contact to the surrounding epithelium. Frequently, large lacunar gaps are present between sperm cells and between sperm and Sertoli cells in knockout epithelium.

Electron microscopy of adult testis of a wild-type mouse is shown. Wild-type Sertoli cytoplasmic arms are broad and tightly wrapped around sperm cells.

Electron microscopy of adult testis of a wild-type mouse is shown. The seminiferous epithelium is firmly packed with sperm cells.<br />

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. Sertoli cells stretch through the entire seminiferous epithelium and attach closely to surrounding sperm cells of different stages.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. Sertoli cells stretch through the entire seminiferous epithelium, wrapping their cytoplasmic arms around surrounding sperm cells.

Electron microscopy of a wild-type mouse testis is shown, demonstrating tight junctions of the basal blood-testis barrier, formed by adjacent Sertoli cells. <br />

Testis histology from a p73 knockout mouse at age P42 is shown by H&E staining. Sexually mature 6-wk old p73KO mice show severe loss of developing sperm cells and mature spermatozoa, creating ‘nearly empty’ seminiferous tubules. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Testis histology from a wild-type adult (P70) mouse is shown by H&E staining. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of adult testis of a wild-type mouse is shown. The seminiferous epithelium is firmly packed with sperm cells attaching very tightly to their Sertoli nurse cell.<br />

Testis histology from an adult p73 knockout mouse is shown by H&E staining. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Immunohistochemical analysis of a wild-type testis for germ cell nuclear antigen 1 (GCNA1, which marks basal spermatogonia) is shown. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Testis histology from an adult isoform-specific TAp73 knockout mouse is shown by H&E staining. TAp73 knockout mice phenocopy global p73 knockout mice, while ΔNp73 deficiency does not affect testis morphology. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Testis histology from an adult (P70) TAp73 knockout mouse is shown by H&E staining. The lumen of the knockout caudal epididymis is either nearly or completely depleted of mature sperm. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of a TAp73 knockout mouse testis is shown. Sertoli cells show defective bilateral junctions, with only one-sided junctional structures and uneven distribution of the anchoring actin bundles along the membrane contact.

Electron microscopy of adult testis of a TAp73 knockout mouse is shown. Knockout Sertoli cells show degenerate morphology with abnormal thin cytoplasmic arms and poor adherence to neighboring sperm cells (large lacunar gaps).

Immunohistochemical analysis of a wild-type testis for proliferation marker Ki67 is shown. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. The wild-type testis displays a tightly packed adhesive seminiferous epithelium with only mature spermatozoa detaching from the apical layer into the lumen.<br />

Electron microscopy of a wild-type mouse testis is shown. The basally located blood-testis barrier (BTB) between adjacent Sertoli cells mainly consists of tight junctions as well as some additional structures (adherens- and desmosomal-like junctions, gap junctions and basal tubulobulbar complex). Wild-type Sertoli-Sertoli cell contacts display bilateral junctions with strictly aligned, directly opposing orientation of the mirrored structural elements. Actin bundles are well developed.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult TAp73 knockout mouse testis is shown. In contrast to wildtype, knockout Sertoli cells reveal degenerate morphology, characterized by cell-cell gaps, thinning of cytoplasmic arms and increased cytoplasmic vacuolization.

Immunohistochemical analysis of a TAp73 knockout testis for proliferation marker Ki67 is shown. TAp73KO testes show normal numbers of proliferating basal spermatogonia. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult p73 knockout mouse testis is shown. In knockout testis late stage developing immature sperm cells fail to properly attach to each other and to Sertoli cells, hence cells lose contact to the surrounding epithelium. Frequently, large lacunar gaps are present between sperm cells and between sperm and Sertoli cells in knockout epithelium.

Electron microscopy of adult testis of a wild-type mouse is shown, including a Sertoli cell, adjoined by sperm cells.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult TAp73 knockout mouse testis is shown. In contrast to wildtype, knockout Sertoli cells reveal degenerate morphology. They show greatly increased cytoplasmic vacuolization.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. The wild-type testis displays a tightly packed adhesive seminiferous epithelium with sperm cells attaching closely to Sertoli cells.

Testis histology from a wild-type mouse at age P42 is shown by H&E staining. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 100x.

Testis histology from an adult isoform-specific ΔNp73 knockout mouse is shown by H&E staining. TAp73 knockout mice phenocopy global p73 knockout mice, while ΔNp73 deficiency does not affect testis morphology. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Immunostaining of testis of an adult TAp73 knockout mouse for Vimentin, an intermediate filament Sertoli cell marker, is shown. TAp73KO Sertoli cells have abnormally short and thin cytoplasmic arms. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Testis histology from a wild-type mouse at age P42 is shown by H&E staining. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of adult testis of a TAp73 knockout mouse is shown. Knockout Sertoli cells show degenerate morphology with high vacuolization.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. The wild-type testis displays a tightly packed adhesive seminiferous epithelium.<br /><br />

Electron microscopy of ultrathin sections from seminiferous tubules of an adult TAp73 knockout mouse testis is shown. In knockout testis late stage developing immature sperm cells fail to properly attach to each other and to Sertoli cells, hence cells lose contact to the surrounding epithelium. Frequently, large lacunar gaps are present between sperm cells and between sperm and Sertoli cells in knockout epithelium.

Immunohistochemical analysis of a TAp73 knockout testis for germ cell nuclear antigen 1 (GCNA1, which marks basal spermatogonia) is shown. TAp73 knockout testes show normal numbers of proliferating basal spermatogonia. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Immunostaining of testis of an adult wild-type mouse for Vimentin, an intermediate filament Sertoli cell marker, is shown. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Testis histology from a p73 knockout mouse at age P42 is shown by H&E staining. Sexually mature 6-wk old p73KO mice show severe loss of developing sperm cells and mature spermatozoa, creating ‘nearly empty’ seminiferous tubules. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 100x.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult wild-type mouse testis is shown. The wild-type testis displays a tightly packed adhesive seminiferous epithelium.

Testis histology from an adult wild-type mouse is shown by H&E staining. The image was acquired on a Zeiss AxioScope A1 with AxioCam MRc using the AxioVision Rel. 4.8 Software. Magnification: 400x.

Electron microscopy of ultrathin sections from seminiferous tubules of an adult TAp73 knockout mouse testis is shown. In knockout testis late-stage developing immature sperm cells fail to properly attach to each other and to Sertoli cells, hence cells lose contact to the surrounding epithelium. Frequently, large lacunar gaps are present between sperm cells and between sperm and Sertoli cells in knockout epithelium.

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