bioimagesUnknownBoubou DiagouragaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201306085JCB

A microtubule tracker (green)-stained platelet is shown during activation with ADP at a final concentration of 135 nM. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 7.23 seconds.

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 50 μM blebbistatin is shown. Platelets were stained with an antibody against tubulin (green).

Microtubule tracker (green)-stained platelets are shown during activation with ADP at a final concentration of 270 nM. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 6.91 seconds. Newly polymerising microtubules starting to reach for the opposite side of the coil can be observed when the marginal band of the right platelet is strongly coiled.

A z-stack (0.25 μm intervals) of confocal planes of a transiently activated platelet present in freshly drawn blood triple stained for acetylated (green), tyrosinated (red), and total (magenta) α-tubulin is shown.

A z-stack (0.25 μm intervals) of confocal planes of a resting platelet triple stained for acetylated (green), tyrosinated (red) and total (magenta) α-tubulin is shown.

A z-stack (0.1 μm intervals) of confocal planes of platelets treated for 3 min with 10 μM of the kinesin inhibitor ATA is shown. Platelets were stained with an antibody against acetylated tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets activated for 60 seconds with 25 nM ADP is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 1 μg/ml cytochalasin D and then activated for 10 minutes with 2.5 μM ADP is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 50 μM blebbistatin and then activated for 10 minutes with 2.5 μM ADP is shown. Platelets were stained with an antibody against tubulin (green).

A microtubule tracker (green)-stained platelet is shown during activation with thrombin at a final concentration of 0.017 U/ml. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 10.5 seconds.

A microtubule tracker (green)-stained platelet is shown during treatment with calyculin at a final concentration of 17 nM. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 7.13 seconds.

A z-stack (0.2 μm intervals) of confocal planes of transiently activated platelets in the absence of dynein inhibition is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets activated for 60 seconds with 6.25 μM arachidonic acid is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of transiently activated platelets after dynein inhibition for 10 minutes with 1 mM EHNA is shown. Platelets were stained with an antibody against tubulin (green). Some platelets have simultaneously a flat and a coiled microtubule ring.

A z-stack (0.25 μm intervals) of confocal planes of a platelet activated for 10 seconds with 5 μM ADP and triple stained for acetylated (green), tyrosinated (red), and total (magenta) α-tubulin is shown.

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 1 μg/ml cytochalasin D is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.25 μm intervals) of confocal planes of a platelet treated for 3 min with 10 μM of the kinesin inhibitor ATA, fixed and triple stained for acetylated (green), tyrosinated (red) and total (magenta) α-tubulin is shown.

A microtubule tracker (green)-stained platelet is shown during inhibition of kinesin with 6.7 μM final concentration of ATA. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 7.3 seconds.

A z-stack (0.1 μm intervals) of confocal planes of a transiently activated platelet present in freshly drawn blood is shown. Platelets were double stained with an antibody against acetylated tubulin (green) and an antibody against beta tubulin (Santa Cruz sc-9104; red).

A z-stack (0.2 μm intervals) of confocal planes of transiently activated platelets after a recovery period is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.25 μm intervals) of confocal planes of a platelet activated for 10 seconds with 5 μM ADP and triple stained for acetylated (green), tyrosinated (red), and total (magenta) α-tubulin is shown. (Only the lower platelet is shown in the published manuscript.)

A z-stack (0.2 μm intervals) of confocal planes of transiently activated platelets present in freshly drawn blood is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 25 μM blebbistatin is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of platelets activated for 10 minutes with 2.5 μM ADP is shown. Platelets were stained with an antibody against tubulin (green).

A z-stack (0.2 μm intervals) of confocal planes of resting platelets is shown. Platelets were stained with an antibody against tubulin (green).

A microtubule tracker (green)-stained platelet is shown during treatment with calyculin at a final concentration of 17 nM. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 7.26 seconds.

A z-stack (0.2 μm intervals) of confocal planes of platelets activated for 60 seconds with 0.01 U/ml thrombin is shown. Platelets were stained with an antibody against tubulin (green).

A microtubule tracker (green)-stained platelet is shown during treatment with calyculin at a final concentration of 17 nM. A z-stack of 6 confocal plans (0.5 μm intervals) was acquired every 7.23 seconds.

A time-lapse video of microtubule tracker (green)-stained platelets spreading on a glass surface is shown. Transmission and fluorescence images are acquired simultaneously every 5 seconds. The platelet shown in Figure 2C and the associated video can be observed in the upper right corner.

A z-stack (0.2 μm intervals) of confocal planes of platelets treated for 30 minutes with 25 μM blebbistatin and then activated for 10 minutes with 2.5 μM ADP is shown. Platelets were stained with an antibody against tubulin (green).

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