{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Caroline Funk"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201310018"],"attach_to":["JCB"],"legend":["Stu1∆ML-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆ML-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","stu1∆TOGL2 S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","Wild-type S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆TOGL1-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆TOGL1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆TOGL1-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆TOGL1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","An intact spindle with Stu1 midzone localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆D4-ZIP-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","An anaphase spindle with the Stu1 D4 domain localizing to the midzone is depicted. Cells expressing D4-GFP in a stu1∆D4-ZIP background were released from G1 arrest and visualized in anaphase. D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","stu1∆TOGL2 S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","Wild-type S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.<br />","An intact spindle with a diffuse Stu1 localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆D4-GFP fails to localize to the midzone of intact anaphase spindles. S. cerevisiae cells expressing Stu1∆D4-GFP in a wind-type background were released from G1 arrest and visualized in anaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","A representative stu1∆D4 cell with a not intact spindle is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Control GFP is shown in green, and CFP-Tub1 is shown in red. Nine Z-stacks were acquired.","An intact spindle with a faint Stu1 midzone localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","stu1∆TOGL1 S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired. <br />","Wild-type S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","An intact spindle with Stu1 along the spindle is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","A not intact spindle with Stu1 localizing along the residual spindle parts is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","A representative cell with an intact spindle is depicted. S. cerevisiae cells expressing T2-D4-GFP in a stu1∆D4 background were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red and T2-D4-GFP in green. Nine Z-stacks were acquired.","Wild-type S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","'∆stu1' S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","'∆stu1' S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","Stu1∆ML-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆ML-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","stu1-TOGL2-4A S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","stu1∆TOGL1 S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","A representative cell with a not intact spindle is depicted. S. cerevisiae cells expressing D4-GFP in a stu1∆D4 background were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red and D4-GFP in green. Nine Z-stacks were acquired.","Stu1∆D4-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆D4-ZIP-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","Stu1∆D4-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.","An anaphase spindle with the Stu1 D4 domain localizing to the midzone is depicted. Cells expressing D4-GFP in a stu1∆D4-ZIP background were released from G1 arrest and visualized in anaphase. D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.<br />","stu1-TOGL2-4A S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.","A not intact spindle with a diffuse Stu1 localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired."],"repository":["bioimages"],"figure_sub":["Figure 8 - E","Image 575120 (Figure 6 - E)","Figure 8 - F","Image 624914 (Figure 2 - E)","Figure 8 - D","Image 575117 (Figure 6 - C)","Image 575119 (Figure 6 - D)","Image 575126 (Figure 8 - D)","Image 624915 (Figure 2 - E)","Image 575111 (Figure 6 - A)","Image 575122 (Figure 8 - D)","Image 575121 (Figure 6 - E)","Image 624874 (Figure 8 - G)","Image 575118 (Figure 6 - D)","Image 575125 (Figure 8 - D)","Image 624871 (Figure 8 - G)","Image 575116 (Figure 6 - C)","Image 575130 (Figure 8 - F)","Figure 8 - G","Image 624912 (Figure 2 - E)","Image 624917 (Figure 2 - E)","Image 575123 (Figure 8 - D)","Image 575128 (Figure 8 - D)","Image 624872 (Figure 8 - G)","Image 624909 (Figure 2 - E)","Image 575124 (Figure 8 - D)","Image 575131 (Figure 8 - F)","Image 624911 (Figure 2 - E)","Image 624913 (Figure 2 - E)","Figure 2 - E","Image 624907 (Figure 2 - E)","Figure 6","Image 575113 (Figure 6 - B)","Figure 8","Image 575129 (Figure 8 - E)","Image 575127 (Figure 8 - D)","Figure 6 - C","Image 624916 (Figure 2 - E)","Figure 6 - D","Image 624908 (Figure 2 - E)","Figure 6 - A","Image 575112 (Figure 6 - B)","Figure 6 - B","Image 575110 (Figure 6 - A)","Image 575132 (Figure 8 - F)","Figure 6 - E","Figure 2"],"pubmed_authors":["Caroline Funk","Verena Schmeiser","Johannes Lechner","Jennifer Ortiz"],"additional_accession":[]},"is_claimable":false,"name":"A TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules","description":null,"dates":{"release":"2014-05-26T11:26:00Z","modification":"2018-11-29T11:26:00Z","creation":"2018-11-29T11:26:00Z"},"accession":"S-JCBD-201310018","cross_references":{"doi":["10.1083/jcb.201310018"]}}