bioimagesUnknownCaroline FunkThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201310018JCB

Stu1∆ML-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆ML-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

stu1∆TOGL2 S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

Wild-type S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆TOGL1-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆TOGL1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆TOGL1-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆TOGL1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

An intact spindle with Stu1 midzone localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆D4-ZIP-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

An anaphase spindle with the Stu1 D4 domain localizing to the midzone is depicted. Cells expressing D4-GFP in a stu1∆D4-ZIP background were released from G1 arrest and visualized in anaphase. D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

stu1∆TOGL2 S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

Wild-type S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.<br />

An intact spindle with a diffuse Stu1 localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆D4-GFP fails to localize to the midzone of intact anaphase spindles. S. cerevisiae cells expressing Stu1∆D4-GFP in a wind-type background were released from G1 arrest and visualized in anaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

A representative stu1∆D4 cell with a not intact spindle is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Control GFP is shown in green, and CFP-Tub1 is shown in red. Nine Z-stacks were acquired.

An intact spindle with a faint Stu1 midzone localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

stu1∆TOGL1 S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired. <br />

Wild-type S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

An intact spindle with Stu1 along the spindle is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

A not intact spindle with Stu1 localizing along the residual spindle parts is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

A representative cell with an intact spindle is depicted. S. cerevisiae cells expressing T2-D4-GFP in a stu1∆D4 background were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red and T2-D4-GFP in green. Nine Z-stacks were acquired.

Wild-type S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

'∆stu1' S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

'∆stu1' S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

Stu1∆ML-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆ML-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

stu1-TOGL2-4A S. cerevisiae cells were released from G1 arrest and visualized in metaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

stu1∆TOGL1 S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

A representative cell with a not intact spindle is depicted. S. cerevisiae cells expressing D4-GFP in a stu1∆D4 background were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red and D4-GFP in green. Nine Z-stacks were acquired.

Stu1∆D4-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆D4-ZIP-expressing S. cerevisiae cells were released from G1 arrest and visualized in metaphase. Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

Stu1∆D4-expressing S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Stu1∆D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

An anaphase spindle with the Stu1 D4 domain localizing to the midzone is depicted. Cells expressing D4-GFP in a stu1∆D4-ZIP background were released from G1 arrest and visualized in anaphase. D4-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.<br />

stu1-TOGL2-4A S. cerevisiae cells were released from G1 arrest and visualized in anaphase. CFP-Tub1 is shown in red, and Ame1-Cherry (kinetochores) in green. Nine Z-stacks were acquired.

A not intact spindle with a diffuse Stu1 localization is depicted. S. cerevisiae cells were released from G1 arrest and visualized in anaphase. Either Stu1-GFP, Stu1∆D4-GFP, or Stu1∆D4-ZIP-GFP is shown in green, CFP-Tub1 in red, and Ame1-Cherry (kinetochores) in blue. Nine Z-stacks were acquired.

bioimagesFigure 8 - EImage 575120 (Figure 6 - E)Figure 8 - FImage 624914 (Figure 2 - E)Figure 8 - DImage 575117 (Figure 6 - C)Image 575119 (Figure 6 - D)Image 575126 (Figure 8 - D)Image 624915 (Figure 2 - E)Image 575111 (Figure 6 - A)Image 575122 (Figure 8 - D)Image 575121 (Figure 6 - E)Image 624874 (Figure 8 - G)Image 575118 (Figure 6 - D)Image 575125 (Figure 8 - D)Image 624871 (Figure 8 - G)Image 575116 (Figure 6 - C)Image 575130 (Figure 8 - F)Figure 8 - GImage 624912 (Figure 2 - E)Image 624917 (Figure 2 - E)Image 575123 (Figure 8 - D)Image 575128 (Figure 8 - D)Image 624872 (Figure 8 - G)Image 624909 (Figure 2 - E)Image 575124 (Figure 8 - D)Image 575131 (Figure 8 - F)Image 624911 (Figure 2 - E)Image 624913 (Figure 2 - E)Figure 2 - EImage 624907 (Figure 2 - E)Figure 6Image 575113 (Figure 6 - B)Figure 8Image 575129 (Figure 8 - E)Image 575127 (Figure 8 - D)Figure 6 - CImage 624916 (Figure 2 - E)Figure 6 - DImage 624908 (Figure 2 - E)Figure 6 - AImage 575112 (Figure 6 - B)Figure 6 - BImage 575110 (Figure 6 - A)Image 575132 (Figure 8 - F)Figure 6 - EFigure 2Caroline FunkVerena SchmeiserJohannes LechnerJennifer OrtizfalseA TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules2014-05-26T11:26:00Z2018-11-29T11:26:00Z2018-11-29T11:26:00ZS-JCBD-20131001810.1083/jcb.201310018