bioimagesUnknownRania S. RizkThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201312039JCB

The timelapse z-series images used to create this video show the microtubule plus-end dynamics in a full-length anaphase spindle in a kip3Δ S. cerevisiae cell arrested in anaphase (cdc15-2, 37˚C). Each timepoint is comprised of 7 z-slices spaced 0.4 μm apart. Time points were collected at 10 sec intervals.

Shown is a fluorescence image of GFP-tubulin-labeled spindles in a field of kip3Δ kip2Δ S. cerevisiae cells arrested in anaphase (cdc15-2, 37 °C) for 3h. The image is presented as a z-stack spaced 0.5 μm apart in order to display the full length and morphology of hyper-elongated spindles from which spindle length data was collected.

Shown is a fluorescence image of GFP-tubulin-labeled spindles in a field of kip3Δ S. cerevisiae cells arrested in anaphase (cdc15-2, 37 °C) for 3h. The image is presented as a z-stack spaced 0.5 μm in order to display the full length and morphology of hyper-elongated spindles. It also clearly shows the intertwining of astral microtubules with spindles that interfere with visualizing and measuring the length of the hyper-elongated spindles.

bioimagesVideo 6Image 623007 (Figure 2 - None)Figure 2 - NoneVideo 6 - NoneImage 623009 (Video 6 - None)Image 623008 (Figure 2 - None)Figure 2Katherine A. DiScipioRania S. RizkMohan L. GuptaKathleen G. ProudfootfalseThe kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization2014-03-10T11:26:11Z2018-11-29T11:26:11Z2018-11-29T11:26:11ZS-JCBD-20131203910.1083/jcb.201312039