{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Meng He"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201401016"],"attach_to":["JCB"],"legend":["An HA-DHHC13 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC9 plasmid was  transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","A GFP-DHHC14 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G; white, anti-ZO-1.","An HA-DHHC17 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","Expression of Luc shRNA was induced with doxycycline in a stable MDCK cell line, and the cells were fixed for immunofluorescence. Green, ankyrin-G; white, ZO-1","A GFP-DHHC8 plasmid was transfected into MDCK cells. The cells were then trypsinzed, grown in low calcium medium, and immunolabeled for GFP-DHHC8 (green) and ankyrin-G (red).","An HA-DHHC20 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","Stable MDCK cells encoding inducible DHHC5 and DHHC8 shRNA were induced with doxycycline and grown in low calcium medium. Cells were then immunolabeled for endogenous ankryrin-G.","An HA-DHHC10 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","Shown is immunostaining of endogenous betaII-spectrin in MDCK cells grown in regular medium.","Shown is immunostaining of endogenous E-cadherin  in MDCK cells grown in regular medium.","Stable MDCK cells encoding inducible Luc shRNA were induced with doxycycline and fixed for immunofluorescence. Green, ankyrin-G; red, betaII-spectrin; white, ZO-1.","An HA-DHHC14 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC21 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","A GFP-DHHC5 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G, white, anti-ZO-1.","Shown is immunostaining of endogenous ankyrin-G in MDCK cells grown in regular medium.","An HA-DHHC2 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC18 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC6 plasmid was  transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An GFP-DHHC8 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G; white, anti-ZO-1.","Stable MDCK cells encoding inducible luc shRNA cells were induced with doxycycline and grown in low calcium medium. Cells were then immunolabeled for endogenous ankryrin-G.","Expression of DHHC5/DHHC8 shRNAs was induced with doxycycline in a stable MDCK cell line, and the cells were fixed for immunofluorescence. Green, ankyrin-G; white, ZO-1.","An HA-DHHC11 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","A GFP-DHHC5 plasmid was transfected into MDCK cells. The cells were then trypsinzed, grown in low calcium medium, and immunolabeled for GFP-DHHC5 (green) and ankyrin-G (red).","An HA-DHHC5 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC3 plasmid was  transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","1ug plasmid DNA encoding GFP was transfected into MDCK cells grown on a 14 mm coverslip, and the cells were fixed for immunofluorescence. Green, GFP; red, betaII-spectrin; white, ZO-1.","A plasmid expressing a chimeric GFP-DHHC5 N terminal/DHHC14 C terminal construct was transfected into MDCK cells and visualized by immunostaining. Green, anti-GFP; white, anti-ZO-1.","An HA-DHHC1 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC15 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC22 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","Shown is immunostaining of endogenous E-cadherin in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.","An HA-DHHC8 plasmid was  transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC12 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC19 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC4 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC7 plasmid was  transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","An HA-DHHC16 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","Shown is immunostaining of endogenous ankyrin-G in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.","Shown is immunostaining of endogenous betaII-spectrin in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.","An HA-DHHC23 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.","1ug plasmid DNA encoding GFP-PLC delta lacking the PH domain was transfected into MDCK cells grown on a 14 mm coverslip, and the cells were fixed for immunofluorescence. Green, GFP; red, betaII-spectrin."],"repository":["bioimages"],"figure_sub":["Image 625265 (Figure 2 - A)","Image 625287 (Figure 5 - A)","Image 625279 (Figure 2 - A)","Image 625511 (Figure 8 - G)","Image 625260 (Figure 2 - A)","Image 625294 (Figure 5 - A)","Figure S4","Image 625258 (Figure 2 - A)","Image 625267 (Figure 2 - A)","Image 625510 (Figure 6 - A)","Image 625272 (Figure 2 - A)","Figure 8 - G","Image 625282 (Figure 2 - D)","Figure S4 - A","Image 625271 (Figure 2 - A)","Image 625277 (Figure 2 - A)","Image 625298 (Figure 5 - C)","Image 625508 (Figure 4 - A)","Image 625270 (Figure 2 - A)","Image 625297 (Figure 5 - B)","Figure 2 - C","Image 625278 (Figure 2 - A)","Figure 2 - D","Image 625299 (Figure 5 - C)","Figure 2 - A","Image 625509 (Figure 4 - A)","Figure 2 - B","Image 625512 (Figure 8 - G)","Image 625290 (Figure 5 - A)","Image 625303 (Figure S4 - A)","Image 625257 (Figure 2 - A)","Image 625266 (Figure 2 - A)","Image 625273 (Figure 2 - A)","Figure 4 - A","Image 625288 (Figure 5 - A)","Image 625264 (Figure 2 - A)","Image 625281 (Figure 2 - C)","Image 625259 (Figure 2 - A)","Image 625293 (Figure 5 - A)","Image 625276 (Figure 2 - A)","Image 625295 (Figure 5 - B)","Image 625263 (Figure 2 - A)","Figure 5 - A","Image 625268 (Figure 2 - A)","Figure 5 - B","Image 625262 (Figure 2 - A)","Figure 5 - C","Image 625275 (Figure 2 - A)","Figure 5","Figure 4","Figure 6","Figure 8","Image 625269 (Figure 2 - A)","Image 625261 (Figure 2 - A)","Image 625280 (Figure 2 - B)","Figure 6 - A","Image 625291 (Figure 5 - A)","Image 625274 (Figure 2 - A)","Figure 2"],"pubmed_authors":["Meng He","Vann Bennett","Khadar M. Abdi"],"additional_accession":[]},"is_claimable":false,"name":"Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assembly","description":null,"dates":{"release":"2014-07-21T11:26:16Z","modification":"2018-11-29T11:26:16Z","creation":"2018-11-29T11:26:16Z"},"accession":"S-JCBD-201401016","cross_references":{"doi":["10.1083/jcb.201401016"]}}