bioimagesUnknownMeng HeThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201401016JCB

An HA-DHHC13 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC9 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

A GFP-DHHC14 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G; white, anti-ZO-1.

An HA-DHHC17 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

Expression of Luc shRNA was induced with doxycycline in a stable MDCK cell line, and the cells were fixed for immunofluorescence. Green, ankyrin-G; white, ZO-1

A GFP-DHHC8 plasmid was transfected into MDCK cells. The cells were then trypsinzed, grown in low calcium medium, and immunolabeled for GFP-DHHC8 (green) and ankyrin-G (red).

An HA-DHHC20 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

Stable MDCK cells encoding inducible DHHC5 and DHHC8 shRNA were induced with doxycycline and grown in low calcium medium. Cells were then immunolabeled for endogenous ankryrin-G.

An HA-DHHC10 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

Shown is immunostaining of endogenous betaII-spectrin in MDCK cells grown in regular medium.

Shown is immunostaining of endogenous E-cadherin in MDCK cells grown in regular medium.

Stable MDCK cells encoding inducible Luc shRNA were induced with doxycycline and fixed for immunofluorescence. Green, ankyrin-G; red, betaII-spectrin; white, ZO-1.

An HA-DHHC14 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC21 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

A GFP-DHHC5 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G, white, anti-ZO-1.

Shown is immunostaining of endogenous ankyrin-G in MDCK cells grown in regular medium.

An HA-DHHC2 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC18 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC6 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An GFP-DHHC8 plasmid was transfected into MDCK cells, and the cells were co-stained for GFP and endogenous ankyrin-G. Green, anti-GFP; red, anti-ankyrin-G; white, anti-ZO-1.

Stable MDCK cells encoding inducible luc shRNA cells were induced with doxycycline and grown in low calcium medium. Cells were then immunolabeled for endogenous ankryrin-G.

Expression of DHHC5/DHHC8 shRNAs was induced with doxycycline in a stable MDCK cell line, and the cells were fixed for immunofluorescence. Green, ankyrin-G; white, ZO-1.

An HA-DHHC11 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

A GFP-DHHC5 plasmid was transfected into MDCK cells. The cells were then trypsinzed, grown in low calcium medium, and immunolabeled for GFP-DHHC5 (green) and ankyrin-G (red).

An HA-DHHC5 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC3 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

1ug plasmid DNA encoding GFP was transfected into MDCK cells grown on a 14 mm coverslip, and the cells were fixed for immunofluorescence. Green, GFP; red, betaII-spectrin; white, ZO-1.

A plasmid expressing a chimeric GFP-DHHC5 N terminal/DHHC14 C terminal construct was transfected into MDCK cells and visualized by immunostaining. Green, anti-GFP; white, anti-ZO-1.

An HA-DHHC1 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC15 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC22 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

Shown is immunostaining of endogenous E-cadherin in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.

An HA-DHHC8 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC12 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC19 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC4 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC7 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

An HA-DHHC16 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

Shown is immunostaining of endogenous ankyrin-G in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.

Shown is immunostaining of endogenous betaII-spectrin in MDCK cells grown in low calcium medium. Polarized MDCK cells were trypsinized and grown in Ca2+-depleted medium for 24 h before fixation.

An HA-DHHC23 plasmid was transfected into MDCK cells and the expressed protein visualized by immunostaining. Red, anti-HA; blue, DAPI.

1ug plasmid DNA encoding GFP-PLC delta lacking the PH domain was transfected into MDCK cells grown on a 14 mm coverslip, and the cells were fixed for immunofluorescence. Green, GFP; red, betaII-spectrin.

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