{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Oluwatobi Ogun"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201404016"],"attach_to":["JCB"],"legend":["Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of neuromasts from whole-mount zebrafish embryos of clarin-1 morphants co-injected with a truncated clarin-1 cRNA. Hair cells were immunostained for pcdh15a (red) and counter-stained for F-actin (green). The truncated version of clarin-1 transcript does not rescue the morphant phenotype.","Shown is an immunohistochemistry analysis conducted 4 days post-fertilization of the anterior crista from whole-mount zebrafish larvae. Hair cells were immunostained for clarin-1 (red) and counter-stained for F-actin (green). Clarin-1 localizes below the cuticular plate and at the fonticulus.","Shown is an immunohistochemistry analysis conducted at 2 days post-fertilization of anterior macula from whole-mount transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos co-injected with clarin-1 cRNA. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synapitc marker MAGUK (green). Transgenic brn3c fish express a membrane-targeted GFP (white) in ear and neuromast hair cells and show normal distribution of ribeye b puncta when co-injected with clarin-1 cRNA.","Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of neuromasts from whole-mount control-injected transgenic (bnr3c:mGFP) zebrafish embryos. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Bnr3c transgenic fish express a membrane-targeted GFP (white) in ear and neuromast hair cells and show basal distribution of ribeye b puncta.","Shown is an immunohistochemistry analysis conducted 4 days post-fertilization of neuromasts from whole-mount zebrafish larvae. Hair cells were immunostained for clarin-1 (red) and the transition zone marker cc2d2a (green). Clarin-1 localizes at the most basal region of this sub-ciliary compartment.","Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of neuromasts from whole-mount transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos. Brn3c transgenic fish were microinjected with the clarin-1 morpholino mixture and immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Bnr3c transgenic fish express a membrane-targeted GFP (white) in ear and neuromast hair cells. Clarin-1 morphants show an increase in the number of ribeye b puncta.","Shown is an immunohistochemistry analysis conducted at 2 days post-fertilization of anterior macula from whole-mount control-injected  transgenic (bnr3c:mGFP) zebrafish embryos. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Bnr3c transgenic fish express a membrane-targeted GFP (white) in ear and neuromast hair cells and show basal distribution of ribeye b puncta.","Shown is an immunohistochemistry analysis conducted 4 days post-fertilization of neuromasts from whole-mount zebrafish larvae. Hair cells were immunostained for clarin-1 (red) and counter-stained with phalloidin for F-actin (green). Clarin-1 localizes below the cuticular plate and at the point of insertion of the kinocilium.","Shown is an immunohistochemistry analysis conducted at 2 days post-fertilization of anterior macula from whole-mount  transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos. Inner ear hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). This transgenic fish line expresses membrane-targeted GFP (white) in ear and neuromast hair cells. Clarin-1 morphants show accumulation of ribeye b puncta at the apical aspect of the hair cells.","Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of neuromasts from whole-mount transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos co-injected with a truncated clarin-1 cRNA. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Transgenic brn3c fish express a membrane-targeted GFP (white) in ear and neuromast hair cells. The truncated version of the clarin-1 transcript does not rescue the morphant phenotype in the transgenic bnr3c zebrafish line.","Shown is an immunohistochemistry analysis conducted 3 days post-fertilization of hair cells from whole-mount zebrafish clarin-1 morphant embryos. Hair cells were immunostained for pcdh15a (red) and counter-stained for F-actin (green). Clarin-1 morphants lack Pcdh15a expression at the apical aspect of neuromast hair cells.","Shown is an immunohistochemistry analysis conducted 3 days post-fertilization of hair cells from whole-mount control-injected zebrafish embryos. Hair cells were immunostained for pcdh15a (red) and counter-stained for F-actin (green).","Shown is an immunohistochemistry analysis conducted 1 day post-fertilization of whole-mount zebrafish embryos. Hair cells were immunostained for clarin-1 (red) and counter-stained for F-actin with phalloidin (green). Clarin-1 is present in both anterior and posterior macular hair cells.","Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of hair cells from whole-mount embryos of clarin-1 morphants co-injected with clarin-1 cRNA. Hair cells were immunostained for pcdh15a (red) and counter-stained for F-actin (green). Pcdh15a apical expression is restored in the cRNA injected morphants.","Shown is an immunohistochemistry analysis conducted at 2 days post-fertilization of anterior macula from whole-mount transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos co-injected with a truncated clarin-1 cRNA. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Bnr3c transgenic fish express a membrane-targeted GFP (white) in ear and neuromast hair cells. The truncated version of the clarin-1 transcript does not rescue the morphant phenotype in the transgenic bnr3c zebrafish line.","Shown is an immunohistochemistry analysis conducted at 3 days post-fertilization of neuromasts from whole-mount transgenic (bnr3c:mGFP) zebrafish, clarin-1 morphant embryos co-injected with clarin-1 cRNA. Hair cells were immunostained for the pre-synaptic marker ribeye b (red) and the post-synaptic marker MAGUK (green). Transgenic brn3c fish express a membrane-targeted GFP (white) in ear and neuromast hair cells and show normal distribution of ribeye b puncta when co-injected with clarin-1 cRNA."],"repository":["bioimages"],"figure_sub":["Figure 8 - F","Figure 8 - C","Image 627190 (Figure 6 - K)","Image 627193 (Figure 8 - I)","Image 627191 (Figure 8 - F)","Image 627157 (Figure 1 - I-B)","Figure 9 - L","Image 627160 (Figure 2 - H)","Figure 9 - F","Image 627192 (Figure 8 - C)","Image 627159 (Figure 1 - I-F)","Figure 9 - I","Image 627158 (Figure 1 - II-D)","Figure 9 - C","Image 627196 (Figure 9 - C)","Figure 1 - II-D","Image 627188 (Figure 6 - G)","Figure 8 - L","Figure 8 - I","Image 627198 (Figure 9 - L)","Image 627189 (Figure 6 - I)","Figure 6 - K","Figure 6 - I","Image 627162 (Figure 6 - C)","Image 627195 (Figure 9 - F)","Image 627197 (Figure 9 - I)","Figure 1 - I-B","Figure 2 - H","Figure 6","Figure 9","Figure 1 - I-F","Figure 8","Figure 6 - C","Image 627194 (Figure 8 - L)","Figure 1","Figure 6 - G","Figure 2"],"pubmed_authors":["Oluwatobi Ogun","Marisa Zallocchi"],"additional_accession":[]},"is_claimable":false,"name":"Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly","description":"Clarin-1 is a four-transmembrane protein expressed by hair cells and photoreceptors. Mutations in its corresponding gene are associated with Usher syndrome type 3, characterized by late-onset and progressive hearing and vision loss in humans. Mice carrying mutations in the clarin-1 gene have hair bundle dysmorphology and a delay in synapse maturation. In this paper, we examined the expression and function of clarin-1 in zebrafish hair cells. We observed protein expression as early as 1 d postfertilization. Knockdown of clarin-1 resulted in inhibition of FM1-43 incorporation, shortening of the kinocilia, and mislocalization of ribeye b clusters. These phenotypes were fully prevented by co-injection with clarin-1 transcript, requiring its C-terminal tail. We also observed an in vivo interact","dates":{"release":"2014-11-03T11:26:22Z","modification":"2018-11-29T11:26:22Z","creation":"2018-11-29T11:26:22Z"},"accession":"S-JCBD-201404016","cross_references":{"doi":["10.1083/jcb.201404016"]}}