bioimagesUnknownZheng WangThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201405026JCB

A time series shows multiple mislocalized F-actin (white; visualized with mCherry::moeABD) patches in the anchor cell of a madd-2 mutant C. elegans. Time points were acquired every minute.

A time series shows F-actin polarity (white; visualized with mCherry::moeABD) stabilized in the anchor cell of an unc-6 (ev400) mutant C. elegans anchor cell towards UNC-6 (magenta; visualized with zmp-5>unc-6::nlg-1 TM::GFP), which is localized to dorsal uterine cell membranes. Time points were acquired at one-minute intervals.

Clusters of UNC-40::GFP (green) colocalize with F-actin (magenta; visualized with mCherry::moeABD) in an unc-6 (ev400) mutant C. elegans anchor cell. Time points were collected at one minute intervals. T=31 here corresponds to t=1' in the published figure.

A time series shows F-actin (white; visualized with mCherry::moeABD) stably polarized at the basal surface of the anchor cell of a wild-type<br />C. elegans. Time points were acquired at one minute intervals.

Shown is a time series of F-actin localization in the anchor cell of an unc-6 C. elegans mutant that revealed cycling between multiple F-actin (white; visualized with mCherry::moeABD) patches and a single cluster. Initially, multiple small F-actin patches formed randomly. After 9 minutes, one patch grew and became dominant. By 26 minutes, this single cluster began disassembling, and multiple new F-actin foci formed. Integrin maintains a light band of F-actin in the anchor cell at the invasive membrane. Time points were acquired at one-minute intervals. T=19 in the viewer corresponds to t=1' in the published figure.

A time series shows dynamic reorientation of F-actin (white; visualized with mCherry::moeABD) polarity towards a changing UNC-6 source (magenta) in an unc-6 mutant C. elegans anchor cell. When the anchor cell made contact with a new anterior source of UNC-6, a polarized response was directed towards this new source and polarity was lost on the posterior dorsal uterine cell as UNC-6 levels diminished. See also Video 4. Time points were acquired at one minute intervals.

bioimagesImage 625895 (Figure 4 - H)Figure 8 - DFigure 8 - AImage 625893 (Figure 6 - A)Image 625836 (Figure 8 - D)Figure 4Image 625850 (Figure 8 - A)Figure 6Figure 8Figure 6 - DImage 626189 (Figure 4 - D)Figure 6 - AFigure 4 - HImage 626187 (Figure 6 - D)Figure 4 - DZheng WangLara M. LindenDavid R. SherwoodJoshua W. ZielKaleb M. NaegeliQiuyi ChiElliott J. HagedornNatasha S. SavagefalseUNC-6 (netrin) stabilizes oscillatory clustering of the UNC-40 (DCC) receptor to orient polarity2014-08-25T11:26:28Z2018-11-29T11:26:28Z2018-11-29T11:26:28ZS-JCBD-20140502610.1083/jcb.201405026