{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Damaris Nadia Lorenzo"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201407063"],"attach_to":["JCB"],"legend":["<br />Shown is a 3D image of white matter tracts in the brain of an AnkB-/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a side view.","Shown are immunofluorescent stainings of detyrosinated alpha-tubulin (red) in control AnkB+/+ mouse neurons.","Shown is the expression and localization of  β2-spectrin  (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in the axons of Pik3c3 flox/flox mouse neurons expressing pBFP.","Shown is a time-lapse imaging series of the distribution of synaptophysin-YFP (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.<br />","Shown are immunofluorescent stainings of detyrosinated alpha-tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.<br />","Shown is a 3D image of white matter tracts in the brain of an heterozygous AnkB+/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view. This is an additional example not presented in the original manuscript.","Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue).","Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing R1194E 220 kDa ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.","Shown is a time-lapse imaging series of the distribution of Rab5-RFP (red) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.","Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) and GFP (green) expression.","Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP.","Shown is a time-lapse imaging series of the distribution of LAMP1-GFP (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.","Shown are immunofluorescent stainings of glutamylated tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.","Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing a polybasic motif mutant  ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.","Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is the expression and localization of total ankyrin-B (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is the expression and localization of  β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is a time-lapse imaging series of the distribution of Rab5-RFP (red) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.","Shown is the expression and localization of  ankyrin-G (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is the expression and localization of total ankyrin-B (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons.","Shown is the expression and localization of ankyrinG  (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons.","Shown is a 3D image of white matter tracts in the brain of a wild-type AnkB+/+ mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view.","Shown is the expression of PIK3C3 (red) in Pik3c3 flox/flox mouse neurons expressing Cre-BFP (blue). <br />This image reveals the loss of PIK3C3 expression and a diffuse staining for the phosphoinositide 3-phosphate biosensor 2xFYVE (green).","Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons.","Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown is a representative image of DIV8 AnkB-wild-type/- mouse hippocampal neurons expressing dsRed (red) and wild-type AnkB-GFP (green). This is an additional example that is not presented in the original manuscript.","Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing WT 220 kDa ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.","Shown is a 3D image of white matter tracts in the brain of an AnkB-/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view.","Shown is a time-lapse imaging series of DD1320AA AnkB-mCherry (red) co-expressed with p62-GFP (green) in axons of neurons from an ankyrin-B null mouse.<br />The interaction of Ankyrin-B with the dynactin subunit p62 through the residues DD1320 recruits the dynein/dynactin complex to organelle membranes. Scale bar: 10 µm.","Shown are immunofluorescent stainings of glutamylated tubulin (red) in control AnkB+/+ mouse neurons.","Shown is a representative image of DIV8 AnkB-wild-type/- mouse hippocampal neurons expressing dsRed (red) and DD1320AA AnkB-GFP (green). This is an additional example that is not presented in the original manuscript.","Shown is a time-lapse imaging series of the distribution of synaptophysin-YFP (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.","Shown are immunofluorescent stainings of acetylated alpha-tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.","Shown is a 3D image of white matter tracts in the brain of a wild-type AnkB+/+ mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a side view.","Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons.","Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red) and  DD1320AA AnkB-GFP (green).","Shown is a time-lapse imaging series of the distribution of LAMP1-GFP (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.","Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.","Shown are immunofluorescent stainings of acetylated alpha-tubulin (red) in control AnkB+/+ mouse neurons.","Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) expression.","Shown is a representative image of DIV8 AnkB+/+ mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) expression."],"repository":["bioimages"],"figure_sub":["Image 627766 (Figure 3 - D)","Image 627761 (Figure 3 - D)","Image 627255 (Figure 4 - D)","Figure 8 - A","Image 627232 (Figure 2 - C)","Figure 8 - B","Image 627781 (Figure 8 - B)","Image 627777 (Figure 8 - B)","Image 627768 (Figure 6 - E)","Image 627225 (Figure 2 - C)","Image 627262 (Figure 4 - D)","Image 627264 (Figure 4 - D)","Figure 9 - B","Figure 9 - C","Image 627209 (Figure 2 - C)","Figure 9 - A","Image 627763 (Figure 3 - D)","Image 627239 (Figure 2 - C)","Image 627299 (Figure 4 - D)","Image 627230 (Figure 2 - C)","Image 627243 (Figure 2 - C)","Image 627772 (Figure 9 - B)","Image 627222 (Figure 2 - C)","Image 627218 (Figure 2 - C)","Image 627235 (Figure 2 - C)","Image 627257 (Figure 4 - D)","Figure 1 - A","Image 627247 (Figure 1 - A)","Image 627764 (Figure 3 - D)","Image 627294 (Figure 4 - D)","Image 627236 (Figure 2 - C)","Image 627219 (Figure 2 - C)","Image 627259 (Figure 4 - D)","Image 627767 (Figure 6 - E)","Image 627248 (Figure 1 - A)","Image 627775 (Figure 9 - C)","Figure 2 - C","Image 627771 (Figure 9 - A)","Image 627765 (Figure 3 - D)","Image 627229 (Figure 2 - C)","Figure 3 - D","Image 627263 (Figure 4 - D)","Image 627224 (Figure 2 - C)","Image 627208 (Figure 2 - C)","Image 627233 (Figure 2 - C)","Image 627240 (Figure 2 - C)","Image 627298 (Figure 4 - D)","Image 627249 (Figure 1 - A)","Image 627762 (Figure 3 - D)","Image 627210 (Figure 2 - C)","Image 627770 (Figure 9 - B)","Image 627256 (Figure 4 - D)","Image 627251 (Figure 1 - A)","Image 627226 (Figure 2 - C)","Image 627261 (Figure 4 - D)","Image 627776 (Figure 5 - E)","Image 627778 (Figure 8 - B)","Image 627291 (Figure 4 - D)","Image 627769 (Figure 6 - E)","Image 627773 (Figure 9 - C)","Figure 4 - D","Image 627265 (Figure 4 - D)","Image 627290 (Figure 4 - D)","Image 627774 (Figure 9 - A)","Image 627250 (Figure 1 - A)","Image 627266 (Figure 4 - D)","Image 627214 (Figure 2 - C)","Image 627221 (Figure 2 - C)","Image 627253 (Figure 4 - D)","Image 627260 (Figure 4 - D)","Image 627227 (Figure 2 - C)","Figure 5 - E","Figure 5","Figure 4","Figure 6","Image 627779 (Figure 8 - A)","Figure 9","Figure 8","Image 627220 (Figure 2 - C)","Image 627254 (Figure 4 - D)","Figure 1","Image 627215 (Figure 2 - C)","Image 627297 (Figure 4 - D)","Figure 3","Figure 6 - E","Figure 2"],"pubmed_authors":["Alexandra Badea","Damaris Nadia Lorenzo","Janell Hostettler","Xiaowei Zhuang","Jiang He","Vann Bennett","Jonathan Davis","Guisheng Zhong"],"additional_accession":[]},"is_claimable":false,"name":"A PIK3C3–Ankyrin-B–Dynactin pathway promotes axonal growth and multiorganelle transport","description":null,"dates":{"release":"2014-12-22T11:26:35Z","modification":"2018-11-29T11:26:35Z","creation":"2018-11-29T11:26:35Z"},"accession":"S-JCBD-201407063","cross_references":{"doi":["10.1083/jcb.201407063"]}}