bioimagesUnknownDamaris Nadia LorenzoThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201407063JCB

<br />Shown is a 3D image of white matter tracts in the brain of an AnkB-/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a side view.

Shown are immunofluorescent stainings of detyrosinated alpha-tubulin (red) in control AnkB+/+ mouse neurons.

Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in the axons of Pik3c3 flox/flox mouse neurons expressing pBFP.

Shown is a time-lapse imaging series of the distribution of synaptophysin-YFP (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.<br />

Shown are immunofluorescent stainings of detyrosinated alpha-tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.<br />

Shown is a 3D image of white matter tracts in the brain of an heterozygous AnkB+/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view. This is an additional example not presented in the original manuscript.

Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue).

Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing R1194E 220 kDa ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.

Shown is a time-lapse imaging series of the distribution of Rab5-RFP (red) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.

Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) and GFP (green) expression.

Shown is the distribution of phosphoinositide 3-phosphate via the 2xFYVE-GFP probe (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP.

Shown is a time-lapse imaging series of the distribution of LAMP1-GFP (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.

Shown are immunofluorescent stainings of glutamylated tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.

Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing a polybasic motif mutant ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.

Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is the expression and localization of total ankyrin-B (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is a time-lapse imaging series of the distribution of Rab5-RFP (red) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.

Shown is the expression and localization of ankyrin-G (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is the expression and localization of total ankyrin-B (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons.

Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons.

Shown is a 3D image of white matter tracts in the brain of a wild-type AnkB+/+ mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view.

Shown is the expression of PIK3C3 (red) in Pik3c3 flox/flox mouse neurons expressing Cre-BFP (blue). <br />This image reveals the loss of PIK3C3 expression and a diffuse staining for the phosphoinositide 3-phosphate biosensor 2xFYVE (green).

Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons.

Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB-/- mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown is a representative image of DIV8 AnkB-wild-type/- mouse hippocampal neurons expressing dsRed (red) and wild-type AnkB-GFP (green). This is an additional example that is not presented in the original manuscript.

Shown is a time-lapse imaging series of mouse AnkB-/- axons co-expressing WT 220 kDa ankyrinB-mCherry (red) and synaptophysin-YFP (green). This series reveals that ankyrinB’s ZU5C domain interacts with membrane phosphoinositide 3-phosphate.

Shown is a 3D image of white matter tracts in the brain of an AnkB-/- mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a top view.

Shown is a time-lapse imaging series of DD1320AA AnkB-mCherry (red) co-expressed with p62-GFP (green) in axons of neurons from an ankyrin-B null mouse.<br />The interaction of Ankyrin-B with the dynactin subunit p62 through the residues DD1320 recruits the dynein/dynactin complex to organelle membranes. Scale bar: 10 µm.

Shown are immunofluorescent stainings of glutamylated tubulin (red) in control AnkB+/+ mouse neurons.

Shown is a representative image of DIV8 AnkB-wild-type/- mouse hippocampal neurons expressing dsRed (red) and DD1320AA AnkB-GFP (green). This is an additional example that is not presented in the original manuscript.

Shown is a time-lapse imaging series of the distribution of synaptophysin-YFP (green) in Pik3c3 flox/flox mouse neurons expressing pCre-BFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.

Shown are immunofluorescent stainings of acetylated alpha-tubulin (red) in AnkB-/- mouse neurons. Ankyrin-B-deficient neurons present normal microtubule dynamics.

Shown is a 3D image of white matter tracts in the brain of a wild-type AnkB+/+ mouse. Brain specimens were actively stained by transcardial perfusion to perform the diffusion tensor imaging analysis and generate the directionally-encoded fractional anisotropy color-map. This image represents a side view.

Shown is the expression and localization of β2-spectrin (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons.

Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red) and DD1320AA AnkB-GFP (green).

Shown is a time-lapse imaging series of the distribution of LAMP1-GFP (green) in Pik3c3 flox/flox mouse neurons expressing pBFP (blue). Together with images from the other panels in Figure 9, this image reveals that neuronal PIK3C3 and phosphoinositide 3-phosphate lipids regulate the transport dynamics of multiple axonal cargos.

Shown is the expression and localization of ankyrinG (red) and MAP2 (green) in Z-sections of DIV8 AnkB+/+ mouse hippocampal neurons. This is an additional example that is not presented in the original manuscript.

Shown are immunofluorescent stainings of acetylated alpha-tubulin (red) in control AnkB+/+ mouse neurons.

Shown is a representative image of DIV8 AnkB-/- mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) expression.

Shown is a representative image of DIV8 AnkB+/+ mouse hippocampal neurons expressing dsRed (red). This is an additional example that is not presented in the original manuscript and also records tubulin (white) expression.

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