bioimagesUnknownMaria S. IoannouThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201407068JCB

Shown are breast epithelial cells expressing a control shRNAmiR. MCF10A cells were transduced with a lentivirus to drive the expression of the shRNAmiR and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet.

Shown are breast epithelial cells expressing an shRNAmiR against DENND2B. MCF10A cells were transduced with a lentivirus to drive the expression of DENND2B shRNAmiR #2 and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet. This image reveals that DENND2B knockdown reduces MCF10A cell invasion.

Shown is the activation of Rab13 during cell migration. Representative ratiometric time-lapse imaging (FRET/mCer3) of Rab13 WT biosensor in a randomly migrating mouse embryonic fibroblast. Cell was imaged every 5 seconds.

Shown is a time-lapse imaging series of active Rab13 distribution in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 Q67L (white) were imaged live every 5 seconds using widefield microscopy. Scale bar represents 10 µm.

Shown is an H&E stain of lung tissue from mice injected with breast cancer cells expressing a shRNA against Rab13. Mice were injected in the tail vein with 1833TR cells stably expressing shRNA Rab13 #1. H&E staining of lung tissue was done 10 weeks following injection. This imaging series reveals that Rab13 knockdown reduces tumor burden in the lungs.

Shown is the co-localization of Rab13, DENND2B and actin at the leading edge of breast epithelial cells. MCF10A cells expressing mCherry-Rab13 (red), GFP-DENND2B (green) and stained with phalloidin-Alexa Fluor 647 (blue) were imaged by confocal microscopy.

Shown is a comparison of active and inactive Rab13 FRET biosensors in mouse embryonic fibroblasts (MEFs). Representative ratiometric images (FRET/mCer3) of Rab13 Q67L biosensor (left) and Rab13 T22N biosensor (right) in MEFs. Scale bars represent 10 μm.

Shown are images from videos of breast epithelial cells expressing RFP (left) or RFP-Rab13 Q67L (right).<br />MCF10A cells were transduced with lentiviruses, grown in Geltrex to form multi-cell spheroids, overlaid with collagen and imaged every hour for 72 hours by phase contrast microscopy. Scale bar represents 50 µm.<br />These images reveal that active Rab13 drives cell invasion.

Shown is the activation of Rab13 at the cell periphery in breast epithelial cells. Representative ratiometric image (FRET/mCer3) of Rab13 WT biosensor in MCF10A cells.

Shown is the distribution of the DENN domain of DENND2B with actin in HeLa cells. HeLa cells expressing the DENN domain (amino acids 687-1121) of GFP-DENND2B (green) were stained with Phalloidin-AF647 (blue) and imaged using confocal microscopy.

Shown is the co-localization of Rab13 and TGN46 in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 (red) and TGN46 labelled with Alexa Fluor 647 (blue) were imaged with confocal microscopy.

Shown is the co-localization of DENND2B and MICAL-L2 in breast epithelial cells. MCF10A cells expressing mCherry-DENND2B (red) and GFP-MICAL-L2 (green) were imaged using confocal microscopy.

Shown is the co-localization of full-length DENND2B and actin in HeLa cells. HeLa cells expressing full-length (amino acids 1-1137) GFP-DENND2B (green) were stained with Phalloidin-AF647 (blue) and imaged using confocal microscopy.

Shown are breast epithelial cells expressing an shRNAmiR against DENND2B. MCF10A cells were transduced with a lentivirus to drive the expression of DENND2B shRNAmiR #1 and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet. This image reveals that DENND2B knockdown reduces MCF10A cell invasion.

Shown is the co-localization of N-terminus DENND2B with actin in HeLa cells. HeLa cells expressing N-terminus (amino acids 1-686) GFP-DENND2B (green) were stained with Phalloidin-AF647 (blue) and imaged using confocal microscopy.

Shown are 1833TR breast cancer cells expressing an shRNA against Rab13. 1833TR cells were transduced with a lentivirus to drive the expression of an shRNA (#1) against Rab13 and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet. This image reveals that Rab13 knockdown reduces cell invasion.

Shown is an H&E stain of lung tissue from mice injected with breast cancer cells expressing a shRNA against Rab13. Mice were injected in the tail vein with 1833TR cells stably expressing shRNA Rab13 #2. H&E staining of lung tissue was done 10 weeks following injection. This imaging series reveals that Rab13 knockdown reduces tumor burden in the lungs.

Shown are 1833TR breast cancer cells expressing an shRNA against Rab13. 1833TR cells were transduced with a lentivirus to drive the expression of an shRNA (#2) against Rab13 and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet. This image reveals that Rab13 knockdown reduces cell invasion.

Shown are breast epithelial cells expressing RFP.<br />MCF10A cells were transduced with a lentivirus to drive the expression of RFP. Cells were fixed and imaged using DIC microscopy.

Shown is the co-localization of ΔC-DENND2B with actin in HeLa cells. HeLa cells expressing ΔC-DENND2B (amino acids 1-1121) GFP-DENND2B (green) were stained with Phalloidin-AF647 (blue) and imaged using confocal microscopy.

Shown is the co-localization of Rab13 and internalized transferrin in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 (red) and internalized transferrin labelled with Alexa Fluor 647 (blue) were imaged with confocal microscopy.

Shown are 1833TR breast cancer cells expressing a control vector. 1833TR cells were transduced with a control lentivirus and plated on Matrigel-coated membranes for 14 h. Cells that invaded through the membranes were fixed and stained with Crystal violet.

Shown is a time-lapse imaging series of inactive Rab13 distribution in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 T22N (white) were imaged live every 2 seconds using widefield microscopy. Scale bar represents 10 µm.

Shown is the distribution of Rab13 and LAMP1 in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 (red) and LAMP1 labelled with Alexa Fluor 488 (green) were imaged with confocal microscopy.

Shown are breast epithelial cells expressing RFP-Rab13 Q67L. MCF10A cells were transduced with a lentivirus to drive the expression of active Rab13. Cells were fixed and imaged using DIC microscopy.

Shown are breast epithelial cells expressing RFP-Rab13 T22N. MCF10A cells were transduced with a lentivirus to drive the expression of inactive Rab13. Cells were fixed and imaged using DIC microscopy.

Shown is the distribution of Rab13 and TIP47 in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 (red) and TIP47 labelled with Alexa Fluor 488 (green) were imaged with confocal microscopy.

Shown is a time-lapse imaging series of Rab13 distribution in breast epithelial cells. MCF10A cells expressing mCherry-Rab13 WT (white) were imaged live every 5 seconds using widefield microscopy. Scale bar represents 10 µm.

Shown is an H&E stain of lung tissue from mice injected with breast cancer cells expressing a control vector. Mice were injected in the tail vein with 1833TR cells stably expressing a control vector. H&E staining of lung tissue was done 10 weeks following injection. This imaging series reveals that Rab13 knockdown reduces tumor burden in the lungs.

bioimagesImage 629313 (Figure 3 - A)Figure 10 - DImage 629364 (Figure 10 - D)Image 629376 (Figure 4 - B)Image 629410 (Figure 6 - B)Image 629371 (Figure 10 - D)Image 629381 (Figure 5 - D)Image 629304 (Figure 2 - A)Image 629322 (Figure 4 - A)Figure 7 - DImage 629314 (Figure 3 - C)Image 629302 (Figure 2 - A)Image 629413 (Figure 7 - D)Figure 9 - AImage 629378 (Figure 5 - C)Image 629415 (Figure 9 - A)Image 629370 (Figure 10 - D)Image 629358 (Figure 10 - D)Figure 2 - IImage 629416 (Figure 9 - A)Image 629373 (Figure 10 - D)Image 629408 (Figure 5 - F)Image 629367 (Figure 10 - D)Figure 2 - DFigure 2 - AImage 629303 (Figure 2 - A)Figure 3 - DFigure 3 - EFigure 3 - FImage 629308 (Figure 2 - I)Figure 3 - AFigure 3 - CImage 629317 (Figure 3 - F)Figure 4 - AImage 629411 (Figure 6 - B)Figure 4 - BImage 629369 (Figure 10 - D)Image 629414 (Figure 9 - A)Image 629359 (Figure 10 - D)Figure 4 - CImage 629360 (Figure 10 - D)Image 629306 (Figure 2 - I)Image 629409 (Figure 6 - B)Image 629368 (Figure 10 - D)Image 629375 (Figure 10 - D)Image 629412 (Figure 6 - B)Image 629363 (Figure 10 - D)Image 629377 (Figure 4 - C)Figure 5 - FImage 629316 (Figure 3 - E)Figure 5 - CFigure 5 - DFigure 5Figure 4Figure 7Figure 6Figure 10Figure 9Image 629315 (Figure 3 - D)Image 629307 (Figure 2 - I)Figure 6 - BImage 629305 (Figure 2 - D)Figure 3Figure 2Jason N.R. HamlinMaria S. IoannouMorag ParkLouis HodgsonMark DaubarasEmily S. BellPeter S. McPhersonMartine GirardAnie MonastMathilde ChaineaufalseDENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior2015-02-23T11:26:37Z2018-11-29T11:26:37Z2018-11-29T11:26:37ZS-JCBD-20140706810.1083/jcb.201407068