{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["FoSheng Hsu"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201408027"],"attach_to":["JCB"],"legend":["Shown is the distribution of GFP-Sac2CS (green) and 594-Tfn (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac3CS and labeled with with Alexa594-Tfn. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-∆hSac2 (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-hSac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Rab11 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab11 and mCherry-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the distribution of DsRed-GalT (red) and GFP-M6PR (green) in mouse neuroblastoma N2A cells. N2A cells were co-transfected with DsRed-GalT and GFP-M6PR. Cells were fixed and imaged. Shown is a single plane section.","Shown is the distribution of the GFP-tagged mutant Sac2 1-768CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 1-768CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2CS (green) and Alexa555-EGF (red) in HeLa cells. Cells were transiently transfected with GFP-Sac2CS and immuno-stained for Alexa555-EGF. Cells were fixed after a 20-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Rab5 (green) and mCherry-Sac2 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab5 and mCherry-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized WT N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed and imaged. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized WT N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed, permeabilized and imaged. Shown is a single plane section.","Shown is the distribution of GFP-OSBP (green) and Alexa647-Tfn (red) in mouse neuroblastoma N2A cells. Cells were transfected with GFP-OSBP (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Rab7 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab7 and mCherry-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2CS (green) and GPP130 (red) in mouse neuroblastoma N2A cells treated with Brefeldin A. N2A cells were transiently transfected with GFP-Sac2CS, treated with Brefeldin A (BFA) for 15 min immediately before fixing and immuno-stained for GPP130. Cells were imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Rab4 (green) and mCherry-Sac2 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab4 and mCherry-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the distribution of integrin β1 in non-permeabilized WT N2A cells. Cells are fixed and stained for mouse β1 integrin (green). Shown is a single plane section.","Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized ∆Sac2 N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed and imaged. Shown is a single plane section.","Shown is the distribution of the GFP-tagged mutant Sac2 1-590CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 1-590CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized ∆Sac2 N2A cells. Cells are fixed and stained for mouse β1 integrin (green). Shown is a single plane section.","Shown is the distribution of GFP-OSBP and Alexa647-Tfn in ∆Sac2 cells expressing mCherry-Sac2. Cells were transfected with GFP-OSBP (green) and mCherry-Sac2 (blue) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2CS (green) and GPP130 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and immuno-stained for GPP130. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells treated with phenylarsine oxide (PAO). N2A cells were transiently transfected with GFP-Sac2 and treated with PAO for 10 minutes. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells treated with DMSO. N2A cells were transiently transfected with GFP-Sac2 and treated with DMSO. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of the catalytically inactive GFP-tagged mutant Sac2CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized WT N2A cells. Cells are fixed, permeabilized, and stained for mouse β1 integrin (green). Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized ∆Sac2 N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed, permeabilized and imaged. Shown is a single plane section.","Shown is the distribution of GFP-Rab9 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and RFP-clathrin-LC. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2CS (green) and RFP-clathrin-LC (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and RFP-clathrin-LC. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of the GFP-tagged mutant Sac2 590-1132CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 590-1132CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in ∆Sac2 cells. Cells were transfected with GFP-2xPH(FAPP1) (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-Sac2CS (green) and Lamp1 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and immuno-stained for Lamp1. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of mCherry-Sac2CS (red) and TfnR (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with mCherry-Sac2CS and immuno-stained for TfnR. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the distribution of DsRed-GalT (red) and GFP-M6PR (green) in ∆Sac2 mouse neuroblastoma N2A cells. ∆Sac2 cells were co-transfected with DsRed-GalT and GFP-M6PR. Cells were fixed and imaged. Shown is a single plane section.","Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in mouse neuroblastoma N2A cells. Cells were transfected with GFP-2xPH(FAPP1) (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.","Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized ∆Sac2 N2A cells. Cells are fixed, permeabilized, and stained for mouse β1 integrin (green). Shown is a single plane section.","Shown is the distribution of GFP-OSBP and Alexa647-Tfn in mouse neuroblastoma N2A cells. Cells were transfected with GFP-OSBP (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.","Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in ∆Sac2 cells expressing mCherry-Sac2. Cells were transfected with GFP-2xPH(FAPP1) (green) and mCherry-Sac2 (blue) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section."],"repository":["bioimages"],"figure_sub":["Figure S2 - F","Figure S2 - D","Figure S2 - E","Image 626101 (Figure 2 - C)","Figure S2 - B","Image 630117 (Figure S2 - B)","Image 630120 (Figure S2 - E)","Image 630106 (Figure 3 - F)","Image 626138 (Figure 5 - D)","Figure S2 - C","Figure S2 - A","Figure 7 - A","Figure 7 - C","Image 630118 (Figure S2 - C)","Image 630169 (Figure 2 - B)","Image 626131 (Figure S2 - F)","Figure S4","Image 626147 (Figure 5 - D)","Figure S2","Image 630109 (Figure 6 - D)","Image 626151 (Figure 5 - D)","Image 626156 (Figure 5 - D)","Image 630116 (Figure S2 - A)","Image 630110 (Figure 6 - D)","Image 626096 (Figure 2 - B)","Figure S4 - A","Image 626152 (Figure 5 - D)","Image 630105 (Figure 3 - E)","Image 626148 (Figure 5 - D)","Image 626099 (Figure 2 - B)","Image 630123 (Figure 7 - C)","Image 626140 (Figure 5 - D)","Image 630125 (Figure 7 - C)","Image 626149 (Figure 5 - D)","Image 630119 (Figure S2 - D)","Image 630104 (Figure 3 - D)","Figure 2 - C","Image 626141 (Figure 5 - D)","Image 630124 (Figure 7 - C)","Figure 2 - B","Figure 3 - D","Figure 3 - E","Image 630168 (Figure 2 - B)","Image 630103 (Figure 3 - C)","Figure 3 - F","Image 626125 (Figure 7 - A)","Image 626155 (Figure 5 - D)","Figure 3 - A","Figure 3 - B","Figure 3 - C","Image 626139 (Figure 5 - D)","Image 630102 (Figure 3 - B)","Image 630112 (Figure 6 - E)","Image 630121 (Figure S4 - A)","Image 626123 (Figure 7 - A)","Image 630108 (Figure 6 - D)","Image 630111 (Figure 6 - D)","Image 630101 (Figure 3 - A)","Image 626157 (Figure 5 - D)","Image 630115 (Figure 6 - E)","Figure 5 - D","Image 626144 (Figure 5 - D)","Image 630113 (Figure 6 - E)","Figure 5","Image 626124 (Figure 7 - A)","Figure 7","Figure 6","Image 630122 (Figure S4 - A)","Image 626100 (Figure 2 - C)","Image 626150 (Figure 5 - D)","Figure 6 - D","Image 630107 (Figure 2 - B)","Image 630167 (Figure 2 - B)","Image 626145 (Figure 5 - D)","Figure 3","Figure 6 - E","Figure 2","Image 630114 (Figure 6 - E)"],"pubmed_authors":["Yuxin Mao","FoSheng Hsu","Fenghua Hu"],"additional_accession":[]},"is_claimable":false,"name":"Spatiotemporal control of phosphatidylinositol 4-phosphate by Sac2 regulates endocytic recycling","description":null,"dates":{"release":"2015-04-13T11:26:42Z","modification":"2018-11-29T11:26:42Z","creation":"2018-11-29T11:26:42Z"},"accession":"S-JCBD-201408027","cross_references":{"doi":["10.1083/jcb.201408027"]}}