bioimagesUnknownFoSheng HsuThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201408027JCB

Shown is the distribution of GFP-Sac2CS (green) and 594-Tfn (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac3CS and labeled with with Alexa594-Tfn. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-∆hSac2 (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-hSac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Rab11 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab11 and mCherry-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the distribution of DsRed-GalT (red) and GFP-M6PR (green) in mouse neuroblastoma N2A cells. N2A cells were co-transfected with DsRed-GalT and GFP-M6PR. Cells were fixed and imaged. Shown is a single plane section.

Shown is the distribution of the GFP-tagged mutant Sac2 1-768CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 1-768CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2CS (green) and Alexa555-EGF (red) in HeLa cells. Cells were transiently transfected with GFP-Sac2CS and immuno-stained for Alexa555-EGF. Cells were fixed after a 20-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Rab5 (green) and mCherry-Sac2 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab5 and mCherry-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized WT N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed and imaged. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized WT N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed, permeabilized and imaged. Shown is a single plane section.

Shown is the distribution of GFP-OSBP (green) and Alexa647-Tfn (red) in mouse neuroblastoma N2A cells. Cells were transfected with GFP-OSBP (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Rab7 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab7 and mCherry-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2CS (green) and GPP130 (red) in mouse neuroblastoma N2A cells treated with Brefeldin A. N2A cells were transiently transfected with GFP-Sac2CS, treated with Brefeldin A (BFA) for 15 min immediately before fixing and immuno-stained for GPP130. Cells were imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Rab4 (green) and mCherry-Sac2 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Rab4 and mCherry-Sac2. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the distribution of integrin β1 in non-permeabilized WT N2A cells. Cells are fixed and stained for mouse β1 integrin (green). Shown is a single plane section.

Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized ∆Sac2 N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed and imaged. Shown is a single plane section.

Shown is the distribution of the GFP-tagged mutant Sac2 1-590CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 1-590CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in non-permeabilized ∆Sac2 N2A cells. Cells are fixed and stained for mouse β1 integrin (green). Shown is a single plane section.

Shown is the distribution of GFP-OSBP and Alexa647-Tfn in ∆Sac2 cells expressing mCherry-Sac2. Cells were transfected with GFP-OSBP (green) and mCherry-Sac2 (blue) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2CS (green) and GPP130 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and immuno-stained for GPP130. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells treated with phenylarsine oxide (PAO). N2A cells were transiently transfected with GFP-Sac2 and treated with PAO for 10 minutes. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2 (green) in mouse neuroblastoma N2A cells treated with DMSO. N2A cells were transiently transfected with GFP-Sac2 and treated with DMSO. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 40 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells expressing Flag-Sac2. Cells were transfected with a vector expressing Flag-S2 (green) and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 20 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of the catalytically inactive GFP-tagged mutant Sac2CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized WT N2A cells. Cells are fixed, permeabilized, and stained for mouse β1 integrin (green). Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized ∆Sac2 N2A cells. Cells are stained for mouse β1 integrin (green) for 1 h at 37 Celsius degrees. Cells were then acid-washed to remove surface antibody-receptor complex and chased in complete medium at 37 Celsius degrees for 20 min to allow recycling of receptor. Cells were fixed, permeabilized and imaged. Shown is a single plane section.

Shown is the distribution of GFP-Rab9 (green) and mCherry-Sac2CS (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and RFP-clathrin-LC. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2CS (green) and RFP-clathrin-LC (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and RFP-clathrin-LC. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of the GFP-tagged mutant Sac2 590-1132CS (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2 590-1132CS. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in ∆Sac2 cells. Cells were transfected with GFP-2xPH(FAPP1) (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-Sac2CS (green) and Lamp1 (red) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with GFP-Sac2CS and immuno-stained for Lamp1. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately before chase (0 min) and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in WT mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 10 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of mCherry-Sac2CS (red) and TfnR (green) in mouse neuroblastoma N2A cells. N2A cells were transiently transfected with mCherry-Sac2CS and immuno-stained for TfnR. Cells were fixed and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the distribution of DsRed-GalT (red) and GFP-M6PR (green) in ∆Sac2 mouse neuroblastoma N2A cells. ∆Sac2 cells were co-transfected with DsRed-GalT and GFP-M6PR. Cells were fixed and imaged. Shown is a single plane section.

Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in mouse neuroblastoma N2A cells. Cells were transfected with GFP-2xPH(FAPP1) (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of transferrin in Sac2-null mouse neuroblastoma N2A cells. Cells were transfected with a control vector expressing Flag and labeled with Alexa647-Tfn (red). Cells were fixed immediately after chase for 5 min and imaged using a confocal microscope. Shown is a single plane section.

Shown is a confocal analysis of the integrin β1 fluorescent signal in permeabilized ∆Sac2 N2A cells. Cells are fixed, permeabilized, and stained for mouse β1 integrin (green). Shown is a single plane section.

Shown is the distribution of GFP-OSBP and Alexa647-Tfn in mouse neuroblastoma N2A cells. Cells were transfected with GFP-OSBP (green) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 5-min chase and imaged using a confocal microscope. Shown is a single plane section.

Shown is the distribution of GFP-2xPH(FAPP1) and Alexa647-Tfn in ∆Sac2 cells expressing mCherry-Sac2. Cells were transfected with GFP-2xPH(FAPP1) (green) and mCherry-Sac2 (blue) and then labeled with Alexa647-Tfn (red). Cells were fixed after a 15-min chase and imaged using a confocal microscope. Shown is a single plane section.

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