bioimagesUnknownLichao LuoThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201409142JCB

Shown is a spectrosome/fusome and GFP staining in a germarium in armi carrying bam.GFP. Flies are raised at room temperature and immunostaining is performed on day 5 after eclosure. DNA is labelled blue, spectrosome/fusome red, GFP green.

Shown is a spectrosome/fusome and GFP staining in c587.gal4>tkvi; UAS-Tkv[EX].GFP. GFP staining represents the expression pattern of Tkv[EX].GFP. Flies are raised at 18 Celsius degrees, switched to 31 degrees after eclosure and dissected on day 4. Spectrosome is stained in red, DNA in blue.

Shown is a Tkv staining in a germarium carrying full tkv8 mutant ECs (GFP-negative cells). Clones are induced by Flp-FRT system while UAS-Flp is diven by c587.gal4. Flies are raised at room temperature and immunostaining is performed on day 4 after eclosure. DNA is labelled blue, Tkv red, GFP green.

Shown is a Tkv staining in a germarium carrying pygo mutant clones. Clones are induced by Flp-FRT system while UAS-Flp is diven by c587.gal4. Flies are raised at room temperature and perfomed immuno staining on day 4 after eclosure. DNA is dyed in blue, Tkv in red, GFP in green.

Shown is a Spectrosome/fusome staining in tkvi in ECs. Flies are raised at 18 degree and dissected on day 5 after eclosure. Spectrosome/fusome is stained in red, vasa in green and DNA in blue.

Shown is a βGal staining in a germarium carrying P2-lacZ. Flies are raised at room temperature and dissected on day 4. Spectrosome is stained in red, DNA in blue, βGal in green.

Shown is Tkv staining in c587.gal4>UAS.GFP. GFP is used to label ECs and part of follicle cells. Flies are raised at room temperature and dissected on day 4. Spectrosome/fusome is stained in blue, GFP in green, tkv in red

Shown is a Spectrosome staining in a germarium with cap cell mutants (lack of GFP signals) for a deficiency removing wg, wnt4, wnt6 and wnt10. lones are induced by Flp-FRT system while UAS-Flp is diven by bab1.gal4. Flies are raised at room temperature and immunostaining was performed on day 9 after eclosure. DNA is labelled blue, Spectrosome red, GFP green.

Shown is in situ hybridization to detect wg mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. wg mRNA is detected by anti RNA probes labeled by Dig. LamC used to label cap cells and TF cells is stained in red, DNA in blue, wg mRNA in green.

Shown is a a Drosophila germarium carrying tkv8 mutant escort cells (ECs). Cells were stained for DNA (blue), alpha-Spectrin (spectrosome, red) and GFP (green). Tkv8 mutant ECs are GFP-negative cells. Clones were induced using the Flp-FRT system, and UAS-Flp is driven by c587.gal4. Flies were raised at room temperature and immunostaining was perfomed on day 4 after eclosure.

Shown is a spectrosome staining in a germarium carrying tkv8 mutant ECs (GFP-negative cells) while overexpressing Tkv[EX]. Clones are induced by Flp-FRT system while UAS-Flp is diven by c587.gal4. Flies are raised at room temperature and mmunostaining is performed on day 4 after eclosure. DNA is dyed in blue, spectrosome in red, GFP in green.

Shown is Tkv antibody staining in c587.gal4>UAS.tkvi. Flies are raised at 18 Celsius degrees, switched to 31 degrees after eclosure and dissected on day 4. Spectrosome/fusome is stained in green, Tkv in red and DNA in blue.

Shown is in situ hybridization to detect wnt2 mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. wnt2 mRNA is detected by anti RNA probes labeled by Dig. LamC used to label cap cells and TF cells is stained in red, DNA in blue, wnt2 mRNA in green.

Shown is a GFP staining in flies carrying Dally GFP. Flies are raised at room temperature and dissected on day 4. GFP is in green. Spectrosome is stained in red, DNA in blue.

Shown is in situ hybridization to detect wnt4 mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. wnt4 mRNA is detected by anti RNA probes labeled by Dig. LamC used to label cap cells and TF cells is stained in red, DNA in blue, wnt4 mRNA in green.

Shown is in situ hybridization to detect tkv mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. Tkv mRNA is detected by anti RNA probes labeled by Dig. Spectromsome is stained in blue, vasa in red, tkv mRNA in green.

Shown is Bam antibody staining in a germarium in pygoi.Flies are raised at room temperature and perfomed immuno staining on day4 after eclosure. DNA is labelled blue, spectrosome/fusome green, Bam red.

Shown is Tkv antibody staining in a germarium carrying one copy of tkv mutant (tkv[8]/Ubi.GFP). Flies are raised at room temperature and immunostaining is performed on day 4 after eclosure. GFP is stained in green, Tkv in red and DNA in blue.

Shown is spectrosome and GFP staining in a germarium containing tkv8 mutant ECs but without mutant cells in cap-cell-contacting ECs (GFP-negative cells). Clones are induced by Flp-FRT system while UAS-Flp is diven by c587.gal4. Flies are raised at room temperature and immunostaining is performed on day 4 after eclosure. DNA is labelled blue, spectrosome red, GFP green.

Shown is a spectrosome/fusome staining in a germarium carrying tkvi in ECs when Bam is overexpressed in germ line cells. Flies are raised at 18 Celsius degrees and switched to 31 degrees after eclosure. Over-expressed Bam is activated by 4 times heat shock (2 times per day) before dissection on day 5 after eclosure. Spectrosome/fusome is stained in red, vasa in green and DNA in blue.

Shown is in situ hybridization to detect wnt6 mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. wnt6 mRNA is detected by anti RNA probes labeled by Dig. LamC used to label cap cells and TF cells is stained in red, DNA in blue, wnt6 mRNA in green.

Shown is in situ hybridization to detect dally mRNA in wild type fly. Flies are raised at room temperature and dissected on day 4. dally mRNA is detected by anti RNA probes labeled by Dig. Spectrosome is stained in red, DNA in blue, dally mRNA in green.

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