bioimagesUnknownKarim El AzzouziThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201510043JCB

MT1-MMP-pHluorin mCherry-Talin-1C.ome.acff

bioimagesFigure 1 - NoneFigure 1Image 635357 (Figure 1 - None)Karim El AzzouziStefan LinderChristiane WiesnerfalseMetalloproteinase MT1-MMP islets act as memory devices for podosome reemergencePodosomes are dynamic cell adhesions that are also sites of extracellular matrix degradation, through recruitment of matrix-lytic enzymes, particularly of matrix metalloproteinases. Using total internal reflection fluorescence microscopy, we show that the membrane-bound metalloproteinase MT1-MMP is enriched not only at podosomes but also at distinct “islets” embedded in the plasma membrane of primary human macrophages. MT1-MMP islets become apparent upon podosome dissolution and persist beyond podosome lifetime. Importantly, the majority of MT1-MMP islets are reused as sites of podosome reemergence. siRNA-mediated knockdown and recomplementation analyses show that islet formation is based on the cytoplasmic tail of MT1-MMP and its ability to bind the subcortical actin cytoskeleton. Collect2016-04-11T11:28:13Z2018-11-29T11:28:13Z2018-11-29T11:28:13ZS-JCBD-20151004310.1083/jcb.201510043