bioimagesUnknownPrzemyslaw A. FilipekThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201703061JCB

Lyspersin KO 230-357-SH Arl8b-EGFP LAMP1

Lyspersin KO HS-1-229 Arl8b-EGFP LAMP1

Lyspersin KO LAMTOR2-VF1 LAMTOR1-VF2 LAMP1

Lyspersin KO Arl8b TN-EGFP LAMP1

Lyspersin KO LAMP1

Lyspersin KO HS-Lyspersin Arl8b QL-EGFP LAMP1

Lyspersin KO Arl8b-EGFP LAMP1

HA-Lyspersin LAMP1

Unedited blots

Lyspersin KO 200-357-SH Arl8b-EGFP LAMP1

Lyspersin KO 1-357-SH Arl8b-EGFP LAMP1

WT Arl8b-EGFP LAMP1

Lyspersin KO HS-Lyspersin Arl8b TN-EGFP LAMP1

LAMTOR2 f/- HS-Lyspersin LAMP1

LAMTOR2 -/- HS-Lyspersin LAMP1

LAMTOR2-/- HA-LAMTOR2 Arl8b-EGFP LAMP1

Lyspersin KO HA-Lyspersin Arl8b-EGFP LAMP1

Lyspersin KO C-domain Lyspersin-VF1Zipper-VF2 LAMP1

Lyspersin KO Zipper-VF1 Zipper-VF2 LAMP1

LAMTOR2-/- Arl8b-EGFP LAMP1

LAMTO1 KO LAMTOR1-HA Arl8b-EGFP LAMP1

Lyspersin KO C-domain Lyspersin-VF1 LAMTOR1-VF2 LAMP1

Lyspersin KO HS-1-200 Arl8b-EGFP LAMP1

WT Arl8b QL-EGFP LAMP1

Lyspersin KO 259-357-SH Arl8b-EGFP LAMP1

Lyspersin KO Arl8b QL-EGFP LAMP1

Lyspersin KO 230-258-SH Arl8b-EGFP LAMP1

Lyspersin KO HS-1-357 Arl8b-EGFP LAMP1

WT LAMP1

LAMTOR2 f/- Arl8b-EGFP LAMP1

Lyspersin KO HS-Lyspersin LAMP1

Lyspersin KO Lyspersin-VF1 LAMTOR1-VF2 LAMP1

unedited blots

Lyspersin KO Lyspersin-VF1 Zipper-VF2 LAMP1

WT LAMP1 LAMTOR1

LAMTOR1 KO Arl8b-EGFP LAMP1

Lyspersin KO HS-1-258 Arl8b-EGFP LAMP1

WT LAMTOR2-VF1 LAMTOR1-VF2 LAMP1

WT Arl8b TN-EGFP LAMP1

HA-lyspersin EEA1

Lyspersin KO LAMP1 LAMTOR1

Lyspersin KO Zipper-VF1 LAMTOR1-VF2 LAMP1

bioimagesImage 642531 (Supplemental Figure 3 - B)Supplemental Figure 5Supplemental Figure 2Supplemental Figure 3Supplemental Figure 1Image 642540 (Supplemental Figure 5 - A)Image 642541 (Figure 1 - C,D)Image 642510 (Figure 4 - A)Image 642514 (Figure 4 - D)Image 642023 (Figure 5 - B)Supplemental Figure 2 - EImage 642515 (Figure 3 - A)Image 642538 (Supplemental Figure 5 - A)Image 642529 (Supplemental Figure 2 - E)Image 642512 (Figure 4 - A)Image 642018 (Figure 5 - A)Supplemental Figure 2 - BImage 642030 (Figure 5 - C)Image 642536 (Supplemental Figure 3 - C)Image 642518 (Figure 6 - C)Image 642026 (Figure 5 - C)Image 642537 (Supplemental Figure 5 - A)Image 642520 (Figure 6 - C)Image 642526 (Supplemental Figure 2 - B)Image 642528 (Supplemental Figure 2 - E)Image 642535 (Supplemental Figure 3 - C)Image 642508 (Figure 1 - B)Figure 1 - C,DImage 642021 (Figure 5 - B)Figure 1 - BImage 642517 (Figure 6 - C)Image 642521 (Figure 7 - A, C)Image 642532 (Supplemental Figure 3 - B)Figure 7 - A, CImage 642020 (Figure 5 - A)Image 642022 (Figure 5 - B)Image 642024 (Figure 5 - C)Image 642539 (Supplemental Figure 5 - A)Figure 2 - A,CImage 642513 (Figure 4 - D)Image 642025 (Figure 5 - C)Figure 3 - AImage 642519 (Figure 6 - C)Figure 4 - AImage 642527 (Supplemental Figure 2 - B)Image 642511 (Figure 4 - A)Image 642509 (Figure 1 - B)Image 642542 (Figure 2 - A,C)Figure 4 - DImage 642028 (Figure 5 - C)Supplemental Figure 3 - BSupplemental Figure 3 - CFigure 5 - ASupplemental Figure 3 - AImage 642533 (Supplemental Figure 3 - B)Image 642523 (Supplemental Figure 1 - A, B, C)Image 642031 (Figure 5 - C)Image 642019 (Figure 5 - A)Image 642032 (Figure 5 - C)Figure 5 - BFigure 5 - CImage 642027 (Figure 5 - C)Figure 5Figure 4Figure 7Image 642530 (Supplemental Figure 3 - A)Image 642029 (Figure 5 - C)Figure 6Image 642534 (Supplemental Figure 3 - C)Figure 6 - CSupplemental Figure 1 - A, B, CFigure 6 - AFigure 1Image 642516 (Figure 6 - A)Image 642543 (Supplemental Figure 2 - B)Supplemental Figure 5 - AFigure 3Figure 2Teodor YordanovCedric H. De SmetPhilipp TschaiknerDaniela EberharterElena L. RudashevskayaPrzemyslaw A. FilipekManuele RebsamenBarbara G. FürnrohrKeiryn L. BennettLeopold KremserGiulio Superti-FurgaStefan LechnerTheresia Dunzendorfer-MattTaras StasykLukas A. HuberKlaus ScheffzekMariana E.G. de AraujoGeorg F. VogelHerbert H. LindnerfalseLAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioningSignaling from lysosomes controls cellular clearance and energy metabolism. Lysosomal malfunction has been implicated in several pathologies, including neurodegeneration, cancer, infection, immunodeficiency, and obesity. Interestingly, many functions are dependent on the organelle position. Lysosomal motility requires the integration of extracellular and intracellular signals that converge on a competition between motor proteins that ultimately control lysosomal movement on microtubules. Here, we identify a novel upstream control mechanism of Arl8b-dependent lysosomal movement toward the periphery of the cell. We show that the C-terminal domain of lyspersin, a subunit of BLOC-1–related complex (BORC), is essential and sufficient for BORC-dependent recruitment of Arl8b to lysosomes. In addi2017-10-09T11:29:17Z2018-11-29T11:29:17Z2018-11-29T11:29:17ZS-JCBD-20170306110.1083/jcb.201703061