{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Guillaume Jacquemet"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201704045"],"attach_to":["JCB"],"legend":["DCIS.COM cells migrating collectively were stained for actin and imaged using an SDC microscope (100x, CMOS camera)","MDA-MB-231 cells transiently expressing mCherry-Myo10 (to visualise filopodia tips) were plated for 2 h on fibronectin (FN), stained for actin and imaged using a total internal reflection fluorescence (TIRF) microscope","Primary rat hippocampal neurons plated on laminin were fixed, stained for actin and MAP2 (neuronal marker) and imaged using an SDC microscope (100x objective, CMOS camera)","MCF10A and DCIS.COM cells were plated in circular invasion assays and left to invade through GFR Matrigel, fibrillar collagen I or media (no overlay) for 3 days. Cells were then fixed, stained for actin and DAPI, and imaged using an SDC microscope (100x objective, CMOS camera)","MCF10A and DCIS.COM cells stably expressing Lifeact-mRFP were plated in circular invasion assays and left to invade through fibrillar collagen I or GFR Matrigel for 3 days before being imaged live using an SDC microscope (100x objective, EMCCD camera) for over 9 h (1 picture every 3 min).","DCIS.COM Lifeact cells were seeded as single cells on GFR Matrigel and allowed to form spheroids for 3 days before being imaged live using an SDC microscope (100x objective, EMCCD camera) for over 3 h (1 picture every 2 min).","DCIS.COM Lifeact cells were injected as single cells into the pericardial cavity of zebrafish embryos and imaged live using an SDC microscope 24 h post injection. Prior to imaging, the embryos were anaesthetize and mounted in low-melting point agarose on glass-bottom dishes. A representative single z plane image of a DCIS.COM Lifeact spheroid growing in the zebrafish embryo pericardial cavity  (63x water objective, CMOS camera)","Transgenic zebrafish embryos (Tg(fli1:EGFP)y1 ; roy -/-; mitfa -/-) were treated with DMSO (1%) or latrunculin B (150 ng/ml) from 25 hpf to 29 hpf. The embryos were then anesthetized and mounted in low-melting point agarose on glass-bottom dishes. Z stack images of the sprouting segmental arteries were obtained live using an SDC microscope (long working distance 63x water objective, CMOS camera).","Transgenic zebrafish embryos (Tg(fli1:EGFP)y1 ; roy -/-; mitfa -/-) were treated with DMSO (1%) or latrunculin B (150 ng/ml) from 25 hpf to 29 hpf. The embryos were then anesthetized and mounted in low-melting point agarose on glass-bottom dishes. Z stack images of the sprouting segmental arteries were obtained live using an SDC microscope (long working distance 63x water objective, CMOS camera)","a DCIS.COM cell plated on fibronectin for 2 h, stained for actin and imaged using a structure illumination microscope","DCIS.COM Lifeact cells were injected as single cells into the pericardial cavity of zebrafish embryos and imaged live using an SDC microscope 24 h post injection (63x water objective, EMCCD camera, 1 frame per 10s). Prior to imaging, the embryos were anaesthetize and mounted in low-melting point agarose on glass-bottom dishes.","MCF10A cells were plated in circular invasion assays and left to invade through media (no overlay) for 3 days. Cells were then fixed, stained for actin and imaged using an SDC microscope (100x objective, CMOS camera)","DCIS.COM cells were seeded were seeded as single cells on GFR Matrigel and left to form spheroids for 21 days, fixed, stained and imaged using a confocal microscope.","DCIS.COM cells were plated in circular invasion assays and left to invade through media (no overlay) for 3 days. Cells were then fixed, stained for F-actin and imaged using an SDC microscope (100x objective, CMOS camera)","DCIS.COM cells were plated in circular invasion assays and left to invade through fibrillar collagen for 3 days. Cells were then fixed, stained for F-actin and imaged using an SDC microscope (100x objective, CMOS camera)","MCF10A and DCIS.COM cells were seeded as single cells on GFR Matrigel and allowed to form spheroids for 5 days, fixed, stained and imaged using an SDC microscope (100x objective, CMOS camera).","A2780 cells transiently expressing mEmerald-Lifeact and migrating on cell-derived matrices (CDMs) in the presence of exogenous FN (labelled with Alexa Fluor 568) were imaged live on an SDC microscope (63x oil objective, EMCCD camera)","NK-92 natural killer cells were seeded on antibody-coated glass (anti-CD18 and anti-NKp30) for 20 minutes before being fixed, stained for actin and imaged by TIRF-SIM.","To analyse filopodia dynamics, DCIS.COM cells stably expressing Lifeact mRFP (DCIS.COM Lifeact) were generated and validated by comparison to parental DCIS.COM cells. Cells were plated in circular invasion assays and left to invade through fibrillar collagen I for 3 days. Cells were then fixed, stained for actin, and imaged using an SDC microscope (100x objective, CMOS camera)."],"repository":["bioimages"],"figure_sub":["Figure 8 - C","Figure 8 - D","Figure 2 - None","Image 642229 (Figure S3 - A)","Image 642240 (Figure S3 - A)","Image 642165 (Figure 5 - B)","Image 642223 (Figure S3 - A)","Figure S3","Image 642254 (Figure S3 - A)","Image 642257 (Figure S3 - A)","Image 642220 (Figure S3 - A)","Image 642177 (Figure 4 - B)","Image 642183 (Figure 7 - C)","Figure S3 - A","Image 642226 (Figure S3 - A)","Image 642251 (Figure S3 - A)","Image 642178 (Figure 6 - A)","Image 642260 (Figure S3 - A)","Image 642174 (Figure 4 - B)","Image 642161 (Figure 4 - B)","Image 642263 (Figure S3 - A)","Image 642201 (Figure S3 - A)","Image 642266 (Figure S3 - A)","Figure 1 - C","Image 642204 (Figure S3 - A)","Image 642269 (Figure 3 - B)","Image 642162 (Figure 7 - B)","Image 642246 (Figure S3 - A)","Image 642231 (Figure S3 - A)","Image 642249 (Figure S3 - A)","Image 642243 (Figure S3 - A)","Image 642237 (Figure S3 - A)","Image 642271 (Figure 2 - None)","Image 642180 (Figure 6 - A)","Image 642234 (Figure S3 - A)","Image 642168 (Figure 5 - B)","Figure 3 - D","Image 642199 (Figure S3 - A)","Image 642252 (Figure S3 - A)","Figure 3 - E","Image 642176 (Figure 4 - B)","Image 642182 (Figure 7 - C)","Image 642169 (Figure 5 - B)","Image 642181 (Figure 6 - A)","Image 642224 (Figure S3 - A)","Figure 3 - A","Image 642193 (Figure S3 - A)","Image 642196 (Figure S3 - A)","Image 642227 (Figure S3 - A)","Figure 3 - B","Image 642221 (Figure S3 - A)","Image 642255 (Figure S3 - A)","Figure 3 - C","Image 642258 (Figure S3 - A)","Image 642166 (Figure 5 - B)","Image 642190 (Figure S3 - A)","Image 642270 (Figure 3 - D)","Image 642272 (Figure 2 - None)","Image 642245 (Figure S3 - A)","Image 642173 (Figure 4 - B)","Image 642157 (Figure 8 - D)","Image 642189 (Figure S3 - A)","Image 642242 (Figure S3 - A)","Image 642186 (Figure S3 - A)","Image 642248 (Figure S3 - A)","Image 642209 (Figure S3 - A)","Image 642206 (Figure S3 - A)","Image 642218 (Figure S3 - A)","Figure 5 - B","Image 642179 (Figure 6 - A)","Figure 5","Figure 4","Image 642171 (Figure 3 - E)","Figure 7","Figure 6","Image 642200 (Figure S3 - A)","Image 642215 (Figure S3 - A)","Image 642212 (Figure S3 - A)","Figure 8","Image 642164 (Figure 5 - B)","Image 642158 (Figure 7 - D)","Image 642203 (Figure S3 - A)","Figure 1","Figure 3","Figure 2","Image 642202 (Figure S3 - A)","Image 642268 (Figure 3 - A)","Image 642205 (Figure S3 - A)","Image 642264 (Figure S3 - A)","Image 642233 (Figure S3 - A)","Image 642236 (Figure S3 - A)","Image 642230 (Figure S3 - A)","Figure 7 - B","Image 642261 (Figure S3 - A)","Figure 7 - C","Image 642187 (Figure S3 - A)","Figure 7 - D","Image 642198 (Figure S3 - A)","Image 642239 (Figure S3 - A)","Image 642208 (Figure S3 - A)","Image 642210 (Figure S3 - A)","Image 642225 (Figure S3 - A)","Image 642195 (Figure S3 - A)","Image 642213 (Figure S3 - A)","Image 642192 (Figure S3 - A)","Image 642216 (Figure S3 - A)","Image 642167 (Figure 5 - B)","Image 642175 (Figure 4 - B)","Image 642219 (Figure S3 - A)","Image 642222 (Figure S3 - A)","Image 642228 (Figure S3 - A)","Image 642217 (Figure S3 - A)","Image 642184 (Figure 8 - C)","Image 642207 (Figure S3 - A)","Figure 4 - B","Image 642238 (Figure S3 - A)","Image 642214 (Figure S3 - A)","Image 642211 (Figure S3 - A)","Image 642244 (Figure S3 - A)","Image 642259 (Figure S3 - A)","Image 642188 (Figure S3 - A)","Image 642256 (Figure S3 - A)","Image 642172 (Figure 1 - C)","Image 642185 (Figure S3 - A)","Image 642232 (Figure S3 - A)","Image 642247 (Figure S3 - A)","Image 642273 (Figure 2 - None)","Image 642235 (Figure S3 - A)","Image 642241 (Figure S3 - A)","Image 642197 (Figure S3 - A)","Image 642194 (Figure S3 - A)","Image 642250 (Figure S3 - A)","Image 642267 (Figure 3 - C)","Image 642253 (Figure S3 - A)","Figure 6 - A","Image 642191 (Figure S3 - A)","Image 642262 (Figure S3 - A)","Image 642265 (Figure S3 - A)"],"pubmed_authors":["Jordan S. Orange","Hellyeh Hamidi","Guillaume Jacquemet","Ilkka Paatero","Artur Padzik","Alexandre F. Carisey","Johanna Ivaska"],"additional_accession":[]},"is_claimable":false,"name":"FiloQuant reveals increased filopodia density during breast cancer progression","description":"Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack of compatible methods to quantify filopodia properties. Here, we present FiloQuant, a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed- and live-cell microscopy data. We demonstrate that FiloQuant can extract quantifiable information, including protrusion dynamics, density, and length, from multiple cell types and in a range of microenvironments. In cellular models of breast ductal carcinoma in situ, we reveal a link between filopodia formation at","dates":{"release":"2017-08-01T11:29:18Z","modification":"2018-11-29T11:29:18Z","creation":"2018-11-29T11:29:18Z"},"accession":"S-JCBD-201704045","cross_references":{"doi":["10.1083/jcb.201704045"]}}