{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Theresia Gutmann"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201711047"],"attach_to":["JCB"],"legend":["(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1T, T-shaped IR in one nanodisc; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 10,619 particles. The class averages were visually assigned to show a T-shaped IR in one nanodisc (1T), an L-shaped monomeric IR (L), or none of these (X). (C) The 202 averages resulting from 5 ISAC generations. The averages shown in Fig. 3 D are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 2U, U-shaped IR in two nanodiscs; 1T, T-shaped IR in one nanodisc; 2T, T-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 13,304 particles. The class averages were visually assigned to show a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), a T-shaped IR in two nanodiscs (2T), an L-shaped monomeric IR (L), or none of these (X). (C) The 286 averages resulting from 13 ISAC generations. The averages shown in Fig. S4 B are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1U, U-shaped IR in one nanodisc; 2U, U-shaped IR in two nanodiscs; 1T, T-shaped IR in one nanodisc; 2T, T-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 10,858 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), a T-shaped IR in two nanodiscs (2T), an L-shaped monomeric IR (L), or none of these (X). (C) The 230 averages resulting from 7 ISAC generations. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1U, U-shaped IR in one nanodisc; 1T, T-shaped IR in one nanodisc; II: II-shaped IR in one nanodisc; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 14,820 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a T-shaped IR in one nanodisc (1T), an II-shaped IR in one nanodisc (II), an L-shaped monomeric IR (L), or none of these (X). (C) The 307 averages resulting from 9 ISAC generations. The averages shown in Fig. 3 C are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1T, T-shaped IR in one nanodisc; 2U, U-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 5503 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), an L-shaped monomeric IR (L), or none of these (X). (C) The 112 averages resulting from 6 ISAC generations. The averages shown in Fig. S4 D are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 2U, U-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 11,493 particles. The class averages were visually assigned to show a U-shaped IR in two nanodiscs (2U), an L-shaped monomeric IR (L), or none of these (X). (C) The 241 averages resulting from 9 ISAC generations. The averages shown in Fig. S4 A are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1T, T-shaped IR in one nanodisc; 2T, T-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 10,239 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), a T-shaped IR in two nanodiscs (2T), an L-shaped monomeric IR (L), or none of these (X). (C) The 166 averages resulting from 3 ISAC generations. The averages shown in Fig. S74C are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1U, U-shaped IR in one nanodisc; 2U, U-shaped IR in two nanodiscs; 1T, T-shaped IR in one nanodisc; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 10,634 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), an L-shaped monomeric IR (L), or none of these (X). (C) The 215 averages resulting from 7 ISAC generations. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 2U, U-shaped IR in two nanodiscs; 1T, T-shaped IR in one nanodisc; 2T: T-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 14,041 particles. The class averages were visually assigned to show a U-shaped IR in two nanodiscs (2U), a T-shaped IR in one nanodisc (1T), a T-shaped IR in two nanodiscs (2T), an L-shaped monomeric IR (L), or none of these (X). (C) The 299 averages resulting from 9 ISAC generations. The averages shown in Fig. 3 B are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm.","(A) Selected area of a raw negative-stain EM image. Some representative particles are circled: 1U, U-shaped IR in one nanodisc; 2U, U-shaped IR in two nanodiscs; L, monomeric IR in one nanodisc. (B) The 200 class averages obtained from K-means classification of 13,800 particles. The class averages were visually assigned to show a U-shaped IR in one nanodisc (1U), a U-shaped IR in two nanodiscs (2U), an L-shaped IR (L), or none of these (X). (C) The 297 averages resulting from 8 ISAC generations. The averages shown in Fig. 3 A are marked with an asterisk. Side length of individual averages in panels B and C: 47.7 nm."],"repository":["bioimages"],"figure_sub":["D6, Orig. Data to Fig 3E","D10, Orig. Data to Fig S4D","Image 643142 (D1, Orig. Data to Fig 3A - None)","D9, Orig. Data to Fig S4C","D2, Orig. Data to Fig 3B and 3E","D5, Orig. Data to Fig 3E - None","Image 643147 (D6, Orig. Data to Fig 3E - None)","D8, Orig. Data to Fig S4B - None","D1, Orig. Data to Fig 3A","D3, Orig. Data to Fig 3C","D4, Orig. Data to Fig 3D - None","D7, Orig. Data to Fig S4A","Image 643143 (D2, Orig. Data to Fig 3B and 3E - None)","D9, Orig. Data to Fig S4C - None","Image 643150 (D9, Orig. Data to Fig S4C - None)","D4, Orig. Data to Fig 3D","Image 643149 (D8, Orig. Data to Fig S4B - None)","D7, Orig. Data to Fig S4A - None","D3, Orig. Data to Fig 3C - None","D8, Orig. Data to Fig S4B","D10, Orig. Data to Fig S4D - None","D5, Orig. Data to Fig 3E","Image 643146 (D5, Orig. Data to Fig 3E - None)","Image 643151 (D10, Orig. Data to Fig S4D - None)","Image 643144 (D3, Orig. Data to Fig 3C - None)","Image 643148 (D7, Orig. Data to Fig S4A - None)","D1, Orig. Data to Fig 3A - None","D2, Orig. Data to Fig 3B and 3E - None","Image 643145 (D4, Orig. Data to Fig 3D - None)","D6, Orig. Data to Fig 3E - None"],"pubmed_authors":["Michal Grzybek","Ünal Coskun","Thomas Walz","Theresia Gutmann","Kelly H. Kim"],"additional_accession":[]},"is_claimable":false,"name":"Visualization of ligand-induced transmembrane signaling in the full-length human insulin receptor","description":"Insulin receptor (IR) signaling plays a critical role in the regulation of metabolism and growth in multicellular organisms. IRs are unique among receptor tyrosine kinases in that they exist exclusively as covalent (αβ)<jats:sub>2</jats:sub> homodimers at the cell surface. Transmembrane signaling by the IR can therefore not be based on ligand-induced dimerization as such but must involve structural changes within the existing receptor dimer. In this study, using glycosylated full-length human IR reconstituted into lipid nanodiscs, we show by single-particle electron microscopy that insulin binding to the dimeric receptor converts its ectodomain from an inverted U-shaped conformation to a T-shaped conformation. This structural rearrangement of the ectodomain propagates to the transmembrane ","dates":{"release":"2018-02-16T11:29:30Z","modification":"2018-11-29T11:29:30Z","creation":"2018-11-29T11:29:30Z"},"accession":"S-JCBD-201711047","cross_references":{"doi":["10.1083/jcb.201711047"]}}