BioModelsapplication/xmlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=curation_notes.txthttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.pdfhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072-biopax2.owlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072-biopax3.owlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072_url.xmlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=manifest.xmlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.scihttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=curation_image.pnghttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=metadata.rdfhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.vcmlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072_url.sedmlhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.pnghttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.mhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072-octave.mhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072-matlab.mhttps://www.ebi.ac.uk/biomodels/model/download/BIOMD0000000072?filename=BIOMD0000000072.odeprimaryOK200Enuo HeManually curatedordinary differential equation modelL2V1https://www.ebi.ac.uk/biomodels/BIOMD000000007212960402falseBioModelsSBMLModelsYi2003 GproteinCycle2003MODEL9468910329Tau-Mu Yi, Hiroaki Kitano, Melvin I SimonTau-Mu Yi12960402, The yeast mating response is one of the best understood heterotrimeric G protein signaling pathways. Yet, most descriptions of this system have been qualitative. We have quantitatively characterized the heterotrimeric G protein cycle in yeast based on direct in vivo measurements. We used fluorescence resonance energy transfer to monitor the association state of cyan fluorescent protein (CFP)-Galpha and Gbetagamma-yellow fluorescent protein (YFP), and we found that receptor-mediated G protein activation produced a loss of fluorescence resonance energy transfer. Quantitative time course and dose-response data were obtained for both wild-type and mutant cells possessing an altered pheromone response. These results paint a quantitative portrait of how regulators such as Sst2p and the C-terminal tail of alpha-factor receptor modulate the kinetics and sensitivity of G protein signaling. We have explored critical features of the dynamics including the rapid rise and subsequent decline of active G proteins during the early response, and the relationship between the G protein activation dose-response curve and the downstream dose-response curves for cell-cycle arrest and transcriptional induction. Fitting the data to a mathematical model produced estimates of the in vivo rates of heterotrimeric G protein activation and deactivation in yeast.. 19, 100. Systems Biology Group, Exploratory Research for Advanced Technology Kitano Symbiotic Systems Project, Japan Science and Technology Corporation, Shibuya, Tokyo, Japan.enuo.he@wolfson.ox.ac.ukUniversity of OxfordBIOMD0000000072The yeast mating response is one of the best understood heterotrimeric G protein signaling pathways. Yet, most descriptions of this system have been qualitative. We have quantitatively characterized the heterotrimeric G protein cycle in yeast based on direct in vivo measurements. We used fluorescence resonance energy transfer to monitor the association state of cyan fluorescent protein (CFP)-Galpha and Gbetagamma-yellow fluorescent protein (YFP), and we found that receptor-mediated G protein activation produced a loss of fluorescence resonance energy transfer. Quantitative time course and dose-response data were obtained for both wild-type and mutant cells possessing an altered pheromone response. These results paint a quantitative portrait of how regulators such as Sst2p and the C-terminal tail of alpha-factor receptor modulate the kinetics and sensitivity of G protein signaling. We have explored critical features of the dynamics including the rapid rise and subsequent decline of active G proteins during the early response, and the relationship between the G protein activation dose-response curve and the downstream dose-response curves for cell-cycle arrest and transcriptional induction. Fitting the data to a mathematical model produced estimates of the in vivo rates of heterotrimeric G protein activation and deactivation in yeast.A quantitative characterization of the yeast heterotrimeric G protein cycle.Yi Tau-Mu TM, Kitano Hiroaki H, Simon Melvin I MIfalseYi2003_GproteinCycle The paper describes both wild-type and mutant cells of G protein cycle by using different values of G protein deactivation. We chosed the wild-type, k=0.11 s-1. The unit of the concentration for the proteins are numbers of molecules per cell. Figure5(A) was reproduced with COPASI 4.0 (Build 18) and SBML_odeSolver. Figure5(B) was reproduced with COPASI 4.0 (Build 18). To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92. 2024-08-212024-09-022006-10-17BIOMD000000007212960402CHEBI:17552CHEBI:15996MODEL9468910329BIOMD0000000072GO:0031684GO:0005623GO:0005834GO:0031680GO:0043235GO:0004934GO:0005102GO:0001664GO:0006412GO:0030163GO:0031683GO:0001965GO:0004930GO:0043241GO:0003924C00035C000444932P34166P34165P06842IPR006742IPR001019